ISSN:
1573-1561
Keywords:
Grain aphid
;
Sitobion avenae
;
Metopolophium dirhodum
;
Rhopalosiphum padi
;
Homoptera
;
Aphididae
;
detoxifying enzymes
;
glutathioneS-transferase
;
resistance
;
cereal allelochemicals
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract The activity and molecular weights of glutathioneS-transferase were studied in three species of cereal aphid:Metopolophium dirhodum, Sitobion avenae, andRhopalosiphum padi. The highest level of glutathioneS-transferase activity was recorded in extracts fromM. dirhodum and the lowest fromS. avenae, and extracts of larvae were more active than those from adults. The activity of this enzyme was higher in extracts ofS. avenae andR. padi previously fed on a moderately resistant wheat variety than on a susceptible variety. Gel filtration followed by SDS-PAGE revealed three protein bands in the active fractions. The first had a molecular weight of 28,500 and the second of 27,500 and were present in all three species of aphid. The third protein differed in the three species, having a molecular weight of 26,000 inS. avenae, 25,500 inM. dirhodum, and 24,000 inR. padi.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02064445
Permalink
