ISSN:
1573-5001
Keywords:
NMR
;
Resonance assignments
;
Heteronuclear two-spin coherence spectroscopy
;
Protein structure determination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary The pulse sequence of a new constant-time 3D triple-resonance experiment, ct-HA[CAN]HN, is presented. This experiment delineates exclusively scalar connectivities and uses 13Cα−15N heteronuclear two-spin coherence to overlay the chemical shift evolution periods of the 13Cα and 15N nuclei, thereby providing the four resonance frequencies of the α-proton, the α-carbon, the amide nitrogen, and the amide proton of a given amino acid residue in three dimensions. This experiment promises to be a valid alternative to 4D experiments, providing the same information on intraresidue polypeptide backbone connectivities in 13C-15N-double-labeled proteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00242481
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