ISSN:
1432-072X
Keywords:
Germ-free mice
;
Intestinal bacteria
;
Mouse
;
Segmented
;
Filamentous bacteria
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A technique is described so that mice mono-associated with non-cultivable, segmented filamentous bacteria (SFB's) can be produced for the first time. As SFB donors, mice were used which had an intestinal microflora consisting of both SFB's and bacteria of the genus Clostridium. Recipients were germ-free mice. It was demonstrated that the intraileal inoculation method was more effective than the orogastric route. Therefore, intestinal homogenates of donor mice were treated with filtered ethanol, diluted and administered intraileally to recipient mice. Evidence is presented that cage mates of the recipient mice were mono-associated with SFB's. The availability of these animals, i.e. in vivo monocultures of SFB's, allows taxonomic and functional characterization of SFB's, which was as yet not possible.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290989
Permalink
