ISSN:
1615-6102
Keywords:
Actin
;
Freeze substitution
;
Fungi
;
Hyphal tip
;
Immunocytochemistry
;
Magnaporthe grisea
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary We have successfully localized fungal actin for the first time using immuno-electron microscopy and hyphal tips of the rice blast pathogenMagnaporthe grisea. Following ultrarapid freezing, samples were processed in a novel substitution fluid of 10% acrolein in anhydrous ethanol and embedded in LR White resin. A monoclonal anti-actin antibody, previously shown to recognizeM. grisea actin, bound specifically to filasomes concentrated in the peripheral cytoplasm of subapical regions, and to the core-region of the Spitzenkörper.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01323344
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