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  • molecular marker sequences  (2)
  • Fish developmental biology  (1)
  • Life and Medical Sciences  (1)
  • 1990-1994  (4)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Analysis of an esterase linked to a locus involved in the regulation of the melanoma oncogene and isolation of polymorphic marker sequences inXiphophorus (1991)
    Springer
    Biochemical genetics 29 (1991), S. 509-524 
    Details
    ISSN: 1573-4927
    Keywords: Xiphophorus ; melanoma ; oncogene regulation ; esterase ; molecular marker sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Melanoma formation inXiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by theTu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecular identification of this regulatory locus (R) requires a precise physical map of the chromosomal region. Therefore we studied esterase isozymes inXiphophorus, two of which have been previously reported to be linked to locusR. We confirm thatES1 is a distant marker forR (approx. 30cM), and contrary to earlier studies, we show that this isozyme is present in all species of the genus and at similar activity levels in all organs tested.ES4, which has also been reported to be linked toR, was found to be a misclassification of liverES1. In an attempt to identify markers that bridge the large distance betweenES1 andR, we have generated DNA probes which are highly polymorphic. They will be useful in finding landmarks on a physical map of theR-containing chromosomal region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554126
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Analysis of an esterase linked to a locus involved in the regulation of the melanoma oncogene and isolation of polymorphic marker sequences inXiphophorus (1991)
    Springer
    Biochemical genetics 29 (1991), S. 509-524 
    Details
    ISSN: 1573-4927
    Keywords: Xiphophorus ; melanoma ; oncogene regulation ; esterase ; molecular marker sequences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Melanoma formation inXiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by theTu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecular identification of this regulatory locus (R) requires a precise physical map of the chromosomal region. Therefore we studied esterase isozymes inXiphophorus, two of which have been previously reported to be linked to locusR. We confirm thatES1 is a distant marker forR (approx. 30cM), and contrary to earlier studies, we show that this isozyme is present in all species of the genus and at similar activity levels in all organs tested.ES4, which has also been reported to be linked toR, was found to be a misclassification of liverES1. In an attempt to identify markers that bridge the large distance betweenES1 andR, we have generated DNA probes which are highly polymorphic. They will be useful in finding landmarks on a physical map of theR-containing chromosomal region.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02426867
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes) (1991)
    Springer
    Molecular genetics and genomics 226 (1991), S. 129-140 
    Details
    ISSN: 1617-4623
    Keywords: Medaka ; Gene transfer ; Transient expression ; DNA fate ; Fish developmental biology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Species of small fish are becoming useful tools for studies on vertebrate development. We have investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporal and spatial expression patterns of bacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3- and 9-month-old adult fish; it was found in high molecular weight form as well as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00273596
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  • 4
    Electronic Resource
    Electronic Resource
    Methodik und Anwendungsgebiete des genetischen Fingerabdruckverfahrens (1994)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 24 (1994), S. 9-14 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Unter einem genetischen Fingerabdruck („DNA-Fingerprint“) versteht man ein mit molekularbiologischen Methoden erstelltes Bandenmuster aus informationsfreien DNA-Abschnitten, welches hochspezifisch für das entsprechende Individuum ist. Die Entdeckung und Weiterentwicklung des genetischen Fingerabdrucks hat innerhalb kürzester Zeit zu erheblichen Fortschritten in den verschiedenen Teildisziplinen der Biologie und Medizin geführt. Durch das Aufzeigen individueller Unterschiede im Erbgut werden mit dieser Methode Grundlagen für die Bearbeitung zahlreicher Fragestellungen geschaffen. Wir wollen in diesem Aufsatz zum einen die Methodik veranschaulichen und zum anderen einige ausgewählte Anwendungsbeispiele vorstellen, die verdeutlichen sollen, welches Potential sich hinter der Methode des „DNA-Fingerprinting“ verbirgt.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19940240103
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