ISSN:
1040-0397
Keywords:
Glucose biosensor
;
Glucose oxidase biocomposite
;
Epoxy-graphite composite
;
Tetrathiafulvalene
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
An inexpensive, robust, and easily mechanized amperometric glucose senor based on a biocomposite material has been constructed. The biocomposite is made of graphite, a nonconductive epoxy resin, tetrathiafulvalene (TTF) as a redox mediator, and glucose oxidase (GOD). The enzyme retains its bioactivity on confinement in the rigid epoxy-graphite matrix, with the oxidation current of the reduced mediator serving as the analytical signal (at 150 mV [vs. Ag/AgCl] in a pH 7.00 buffered solution featuring 0.1 M phosphate and 0.1 M KC1). The resulting biosensor shows a linear response in the 0.1 to 2 mM glucose range and produces a steady-state signal within a few seconds (11 seconds for a 95% response). Simple polishing procedures can be used to regenerate a freshly bioactive transducer surface.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140060908
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