Publication Date:
1994-08-26
Description:
DNA replication in the budding yeast Saccharomyces cerevisiae initiates from origins of specific DNA sequences during S phase. A screen based on two- and one-hybrid approaches demonstrates that the product of the DBF4 gene interacts with yeast replication origins in vivo. The Dbf4 protein interacts with and positively regulates the activity of the Cdc7 protein kinase, which is required for entry into S phase in the yeast mitotic cell cycle. The analysis described here suggests a model in which one function of Dbf4 may be to recruit the Cdc7 protein kinase to initiation complexes.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dowell, S J -- Romanowski, P -- Diffley, J F -- New York, N.Y. -- Science. 1994 Aug 26;265(5176):1243-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8066465" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
*Cell Cycle Proteins
;
*DNA Replication
;
DNA, Fungal/biosynthesis
;
Enzyme Activation
;
Fungal Proteins/genetics/*metabolism
;
Models, Biological
;
Molecular Sequence Data
;
Point Mutation
;
Protein Kinases/genetics/*metabolism
;
*Protein-Serine-Threonine Kinases
;
Replicon
;
S Phase
;
Saccharomyces cerevisiae/cytology/enzymology/genetics/*metabolism
;
*Saccharomyces cerevisiae Proteins
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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