Publication Date:
1994-01-28
Description:
Activation of the phospholipase D (PLD) pathway is a widespread response when cells are activated by agonists that bind receptors on the cell surface. A 16-kD cytosolic component can reconstitute guanosine triphosphate (GTP)-mediated activation of phospholipase D in HL60 cells depleted of their cytosol by permeabilization. This factor was purified and identified as two small GTP-binding proteins, ARF1 and ARF3. Recombinant ARF1 substituted for purified ARF proteins in the reconstitution assay. These results indicate that phospholipase D is a downstream effector of ARF1 and ARF3. The well-established role of ARF in vesicular traffic would suggest that alterations in lipid content by PLD are an important determinant in vesicular dynamics.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cockcroft, S -- Thomas, G M -- Fensome, A -- Geny, B -- Cunningham, E -- Gout, I -- Hiles, I -- Totty, N F -- Truong, O -- Hsuan, J J -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1994 Jan 28;263(5146):523-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Physiology, University College London, United Kingdom.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8290961" target="_blank"〉PubMed〈/a〉
Keywords:
ADP-Ribosylation Factor 1
;
ADP-Ribosylation Factors
;
Amino Acid Sequence
;
Animals
;
Cattle
;
Cytosol/chemistry
;
Enzyme Activation
;
GTP-Binding Proteins/chemistry/isolation & purification/*metabolism
;
*Glycerophospholipids
;
Granulocytes/*metabolism
;
Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology
;
Humans
;
Molecular Sequence Data
;
Phosphatidic Acids/metabolism
;
Phosphatidylcholines/metabolism
;
Phospholipase D/*metabolism
;
Recombinant Proteins/metabolism
;
Tumor Cells, Cultured
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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