ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Hits per page

hit 1 - 1 | 1 hit

Sorting

Electronic Resource

Cell culture for three-dimensional modeling in rotating-wall vessels: An application of simulated microgravity (1992)

Schwarz, Ray P. ; Goodwin, Thomas J. ; Wolf, David A.

Springer

Methods in cell science 14 (1992), S. 51-57

add to mindlist on the mindlist

Details

ISSN: 1573-0603

Keywords: three-dimensional cell culture ; microcarrier cell culture ; BHK cells ; high-density cell culture ; rotating-wall vessel ; bioreactor

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary High-density, three-dimensional cell cultures are difficult to grow in vitro. The rotating-wall vessel (RWV) described here has cultured BHK-21 cells to a density of 1.1 × 107 cells/ml. Cells on microcarriers were observed to grow with enhanced bridging in this batch culture system. The RWV is a horizontally rotated tissue culture vessel with silicon membrane oxygenation. This design results in a low-turbulence, low-shear cell culture environment with abundant oxygenation. The RWV has the potential to culture a wide variety of normal and neoplastic cells.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01404744

Permalink

	Location	Call Number	Expected	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

hit 1 - 1 | 1 hit