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  • Polymer and Materials Science  (4)
  • oleosin  (3)
  • Atmospheric circulation
  • Australia
  • Biochemistry and Biotechnology
  • Water budget
  • 1990-1994  (7)
  • 1
    Digitale Medien
    Digitale Medien
    Sequence of an oleocin cDNA from Brassica napus (1992)
    Springer
    Plant molecular biology 19 (1992), S. 1079-1083 
    Details
    ISSN: 1573-5028
    Schlagwort(e): oleosin ; embryogenesis ; cDNA ; Brassica napus ; oil-body protein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Antibodies raised against purified rapeseed 19 kDa oleosin protein were used to screen an embryo-derived λgt11 expression library from Brassica napus. A near full-length cDNA clone, BnV, was isolated. The 781 bp cDNA contained an open reading frame of 549 bp followed by an untranslated region of 222 pb and a poly(A) region of 10 bp. Comparisons between this cDNA and a different oleosin cDNA previously isolated from the same library showed high degrees of sequence similarity in the central domain region and in the 3′ untranslated region. Sequence similarities between the derived protein sequence of this cDNA and all other known oleosin protein sequences are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00040541
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  • 2
    Digitale Medien
    Digitale Medien
    Cloning and characterisation of an oleosin gene from Brassica napus (1992)
    Springer
    Plant molecular biology 19 (1992), S. 443-453 
    Details
    ISSN: 1573-5028
    Schlagwort(e): Brassica napus ; embryogenesis ; leucine-zipper motif ; oleosin ; oil-body protein ; seed development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The sequence of an oleosin gene from Brassica napus has been determined. This gene contains a single intron of 437 bp and encodes a polypeptide of 195 amino acids. The oleosin gene product has an estimated molecular mass of 21.5 kDa and consists of a highly hydrophobic central domain flanked by relatively polar N- and C-terminal domains. The central domain is highly conserved between all oleosins sequenced to date and contains a run of periodically spaced leucine residues similar to that of a leucine-zipper motif. The gene has been shown to be expressed specifically in the embryo, maximally between 9 and 11 weeks after flowering, i.e. during the seed desiccation stage. Two transcriptional start sites have been mapped to -70 and -21 of the ATG and a putative ABA-responsive element and three repeated motifs have been identified in the promoter. These short promoter sequences could correspond to regulatory elements responsible for embryo-specific gene expression. Up to six genes exist in the oleosin gene family.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00023392
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  • 3
    Digitale Medien
    Digitale Medien
    A seed-specific Brassica napus oleosin promoter interacts with a G-box-specific protein and may be bi-directional (1994)
    Springer
    Plant molecular biology 24 (1994), S. 327-340 
    Details
    ISSN: 1573-5028
    Schlagwort(e): abscisic acid ; ABA-response element ; bi-directional promoter ; Brassica napus ; oleosin ; seed development
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In Brassica napus, oleosins are expressed at high levels in the seed during the latter stages of embryo development. The cis-acting regulatory properties of an 872 bp promoter fragment of a B. napus oleosin gene were examined by analysis of β-glucuronidase (GUS) expression in transgenic tobacco plants containing an oleosin promoter-GUS transcriptional fusion. The reporter gene was expressed at high levels only in seeds, specifically in embryo and endosperm tissue and regulated throughout seed development. These data demonstrate that oleosin gene transcription is regulated in a tissue-specific and temporally regulated manner and clearly indicate that oleosin protein expression is co-ordinated primarily at the transcriptional level. Oleosin mRNA was shown to be abscisic acid (ABA) inducible and an ABA-response element in the oleosin promoter was shown to be bound by a protein factor in a sequence-specific manner. Sequence analysis of the oleosin promoter has identified several other putative cis-acting sequences which may direct oleosin gene expression. The presence of a large open reading frame in the bottom strand of the oleosin promoter (ORF2) which encodes a polypeptide similar to the ethylene-induced E4 gene of tomato is reported. A PCR-generated DNA probe containing the ORF2 sequence hybridised with a 1.4 kb transcript in total RNA extracts of a variety of tissues, including leaves and germinated seed cotyledons. This finding suggests that the oleosin gene promoter directs transcription in both directions. It is the first report of a bi-directional nuclear gene promoter in plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00020171
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  • 4
    Digitale Medien
    Digitale Medien
    Measurement of the interfacial tension between polyethersulfone and a thermotropic liquid-crystalline copolyester (1993)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 31 (1993), S. 221-227 
    Details
    ISSN: 0887-6266
    Schlagwort(e): interfacial tension ; thermotropic ; copolyester ; polyether sulphone ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Due to the increasing interest in forming blends of liquid-crystalline polymers with conventional thermoplastics, it becomes important to determine the interfacial tension between two such polymers. A method for evaluating the interfacial tension between a thermotropic copolyester based on hydroxybenzoic and hydroxynaphthoic acid residues, and polyethersulfone is presented, based on the Fort and Patterson method. It is found that the value of the interfacial tension in the melt is much higher than is the case between conventional polymer pairs. It is suggested that this high value reflects an entropic effect due to the strong exclusion of the flexible coil polymer from the nematic melt. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/polb.1993.090310211
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  • 5
    Digitale Medien
    Digitale Medien
    Extensive porosity at the cement-femoral prosthesis interface: A preliminary study (1993)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 27 (1993), S. 71-78 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: This study is the first description of the extensive porosity which is preferentially located at the cement-prosthesis interface of cemented femoral components of total hip replacements. The observation is important because the interfacial porosity may decrease the strength of the cement-femoral prosthesis interface and jeopardize the mechanical integrity of the cement mantle. We examined the cement-metal interfaces from a multiplicity of in vivo and in vitro specimens using both optical and scanning electron microscopy. These samples included several stem designs, implants made from either Co-Cr or Ti alloy, implants made with a variety of surface finishes and both centrifuged and uncentrifuged cement. All in vivo and in vitro samples had marked porosity in the cement focally concentrated at the cement-metal interface. The amount of porosity at the interface greatly exceeded the amount of general porosity found throughout the bulk cement. Centrifuging did not affect the interfacial porosity, and neither did alloy nor surface finish. The presence of these pores may be expoained by the rheological characteristics of the cement. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820270110
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  • 6
    Digitale Medien
    Digitale Medien
    Comparison of 316LVM and MP35N alloys as charge injection electrodes (1994)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 28 (1994), S. 233-240 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: An In vitro comparison of the corrosion response of 316LVM stainless steel and MP35N (a CoNiCrMo alloy) electrodes under conditions appropriate to applications in functional electrical stimulation (FES) was made. Electrodes of both alloys were subjected to a cathodic 40 μC/cm2 charge injection protocol and the potential transient response was recorded over a 96 h period. The transient responses were compared with potentiodynamic polarization data used to establish the quasiequilibrium response of the alloys in the carbonate and phosphate-buffered saline electrolyte used in the study. The MP35N electrodes exhibited extensive pitting corrosion during charge injection, whereas little corrosion was observed on 316LVM electrodes. An explanation for the susceptibility of MP35N to corrosion during charge injection is found in the potentiodynamic polarization data, which reveal a breakdown potential (critical pitting potential) of 0.45 V (SCE) for MP35N compared with 1.05 V (SCE) for 316LVM. Factors that may influence corrosion response during charge injection from alloys exhibiting active-passive behavior are discussed. © 1994 John Wiley & Sons, Inc.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820280214
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  • 7
    Digitale Medien
    Digitale Medien
    Effect of vacuum mixing on the mechanical properties of antibiotic-impregnated polymethylmethacrylate bone cement2 (1990)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 24 (1990), S. 573-580 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Polymethylmethacrylate bone cement, containing either no added antibiotic, 0.5 g of Vancomycin, 1.0 g of Vancomycin, or 1.0 g of Tobramycin, was mixed either in air or a vacuum chamber. Following storage in a water bath at 37°C for 48 h, the specimens were tested in four-point bending. The porosity of the specimens was assessed radiographically, and their antibacterial activity was monitored for 21 days. The bending strength of the vacuum mixed specimens containing no antibiotic was 40% greater than that of similar air-mixed specimens. However, there were no significant differences in the bending strength of either the air-or vacuum-mixed specimens when any of the antibiotic dosages were added. The bending modulus of the vacuum-mixed specimens, containing no antibiotic, was significantly greater than the moduli of all the other specimen groups which did not differ from each other. Vacuum mixing reduced the apparent porosity of the specimens fivefold, and while the addition of antibiotic did not effect porosity of the air-mixed specimens, that of the vacuum-mixed specimens was doubled. Although initial rapid decreases were seen, leaching of antibiotic from the cement and antibacterial activity continued through the 21-day monitoring period.
    Zusätzliches Material: 2 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820240504
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