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  • Earth Resources and Remote Sensing  (1)
  • chloroplast  (1)
  • Animals
  • Arabidopsis thaliana
  • 1990-1994  (2)
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Biosynthesis of active spinach-chloroplast thioredoxin f in transformed E. coli (1992)
    Springer
    Plant molecular biology 20 (1992), S. 301-306 
    Details
    ISSN: 1573-5028
    Keywords: chloroplast ; Escherichia coli ; overexpression ; purification ; Spinacia oleracea ; thioredoxin f
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recently cloned gene for spinach chloroplast thioredoxin f was subcloned in a modified pKK233-2 expression vector and used for transformation of Escherichia coli cells containing the Iq plasmid. After induction with IPTG (isopropyl-β-D-thiogalactoside) the transformed cells produce the chloroplast protein in large amounts as insoluble deposit within the cell. The protein has been solubilized, purified and analysed for activity. It shows no difference in catalytic activity from native spinach chloroplast thioredoxin f. Its electrophoretic behaviour suggests that the native thioredoxin f may have a different N-terminus than was assumed on the basis of the protein sequencing results.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014497
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    Paper (German National Licenses)
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  • 2
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    An Improved Automatic Classification of a Landsat/TM Image from Kansas (FIFE) (1994)
    In:  CASI
    Details
    Publication Date: 2019-07-10
    Description: This research note shows the results of applying a new massively parallel version of the automatic classification program (AutoClass IV) to a particular Landsat/TM image. The previous results for this image were produced using a "subsampling" technique because of the image size. The new massively parallel version of AutoClass allows the complete image to be classified without "subsampling", thus yielding improved results. The area in question is the FIFE study area in Kansas, and the classes AutoClass found show many interesting subtle variations in types of ground cover. Displays of the spatial distributions of these classes make up the bulk of this report. While the spatial distribution of some of these classes make their interpretation easy, most of the classes require detailed knowledge of the area for their full interpretation. We hope that some who receive this document can help us in understanding these classes. One of the motivations of this exercise was to test the new version of AutoClass (IV) that allows for correlation among the variables within a class. The scatter plots associated with the classes show that this correlation information is important in separating the classes. The fact that the spatial distribution of each of these classes is far from uniform, even though AutoClass was not given information about positions of pixels, shows that the classes are due to real differences in the image.
    Keywords: Earth Resources and Remote Sensing
    Type: FIA-94-01
    Format: text
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    NASA TECHNICAL REPORTS
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