ISSN:
1612-1112
Keywords:
Affinity chromatography
;
Affinity electrophoresis
;
Bile acid transport proteins
;
Bile acid derivatives
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary For the purification of the bile acid transport proteins from rat hepatocytes high affinity taurocholic acid derivatives were synthesized. One type was designed for coupling to a Sepharose matrix and the other for copolymerization with acrylamide. The resulting matrices were found to be suitable for affinity chromatography and affinity electrophoresis. In both syntheses, 3α, 12α-dihydroxy-7β-amino (tauro) cholic acid derivatives were used as the starting material, since substitution in this position had previously been shown not to alter transport affinity constant.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02276251
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