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  • 6-phosphogluconate dehydrogenase
  • hairy roots
  • 1
    Electronic Resource
    Electronic Resource
    Establishment of hairy root cultures ofDatura stramonium. Characterization and stability of tropane alkaloid production during long periods of subculturing (1993)
    Springer
    Plant cell, tissue and organ culture 33 (1993), S. 321-329 
    Details
    ISSN: 1573-5044
    Keywords: Datura stramonium ; hairy roots ; hyoscyamine ; tropane alkaloids ; scopolamine ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of this paper was the screening of the variability of growth patterns, biomass and tropane alkaloid production of 500 hairy root lines ofDatura stramonium. Data on the long term stability in alkaloid production of these lines for more than 5 years are also provided. In an effort to obtain high alkaloid-producing root clones, it is demonstrated that systematic selection is necessary. Comparisons are made, mainly concerning alkaloid production and its stability, with normal root cultures initiated from the same mother plants when necessary. Hairy root cultures were found to have a hyoscyamine and scopolamine bioproductivity of 2 orders of magnitude higher than mother plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02319018
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  • 2
    Electronic Resource
    Electronic Resource
    Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures (1994)
    Springer
    Plant cell, tissue and organ culture 38 (1994), S. 273-279 
    Details
    ISSN: 1573-5044
    Keywords: Ajmalicine ; alkaloids ; catharanthine ; Catharanthus roseus ; hairy roots
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two year old, transformed root cultures of Catharanthus roseus accumulate ajmalicine and catharanthine (0.57 and 0.36 mg g-1 DW, or 7.0 and 3.0 mg l-1, respectively). Changes in the concentration of the medium components, as well as the addition of hydrolytic enzymes and biotic elicitors, were used as strategies to increase these alkaloid yields. Regarding the components of the medium, the results obtained, when sucrose was raised from 3 to 4.5%, are noteworthy. The nitrogen source induced differential responses in the individual alkaloid yields. No net change in the alkaloid content was observed either with changes in the concentration of vitamins or macro-and micronutrients. Though the root culture only shows a limited response to elicitors, Aspergillus treatment and the use of macerozyme increased the accumulation of ajmalicine selectively, while the addition of methyl jasmonate increased the yield of both alkaloids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033887
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic stability of hairy root cultures of Datura stramonium (1999)
    Springer
    Plant cell, tissue and organ culture 59 (1999), S. 9-17 
    Details
    ISSN: 1573-5044
    Keywords: Datura stramonium ; genetic stability ; hairy roots ; karyotype ; organ culture ; tropane alkaloids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The karyotypic status of three root lines, transformed with Agrobacterium rhizogenes, versus a non-transformed root line of Datura stramonium, as well as grown plants (control), was investigated. In the transformed cultures, the karyotype as well as the number of chromosomes (2n = 24) remained the same as in the control plants. In the normal root line there were clear differences at a morphological level despite showing the same chromosomic number (2n = 24) at a high frequency. The existence of aneuploids (2n = 2n ± x) and tetraploids (2n = 4x = 48) was also observed in the latter. The frequency of chromosomic alterations was very low in all the cultures. Cytogenetic analysis of the transformed root cultures of D. stramonium reported here shows a great chromosomic stability, which is not the case with normal root cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006398727508
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  • 4
    Electronic Resource
    Electronic Resource
    Long-term adaptive response to dietary protein of hexose monophosphate shunt dehydrogenases in rat kidney tubules (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 8 (1990), S. 11-17 
    Details
    ISSN: 0263-6484
    Keywords: Glucose 6-phosphate dehydrogenase ; 6-phosphogluconate dehydrogenase ; isolated kidney tubules ; dietary regulation ; kinetic analysis ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have studied the effects of several different macronutrients on the kinetic behaviour of rat renal glucose 6-phosphate dehydrogenase (G6PDH) and 6-phosphogluconate dehydrogenase (6PGDH). Rats were meal-fed with high-carbohydrate/low-protein, high-protein/low-carbohydrate and high-fat diets. High-protein increased renal G6PDH and 6PDGH activities by 66 per cent and 70 per cent respectively, without significantly changing the Km values of either and each Hexose monophosphate dehydrogenase activity increased steadily, reaching a significant difference on day 4. A rise in carbohydrate or fat in the diets, produced no significant change in either the activity or the kinetic parameters, Vmax and Km of the two dehydrogenases. In addition, the administration of a high-protein diet for 8 days significantly increased both the pentose phosphate pathway flux (92·6 per cent) and the kidney weight (35 per cent), whereas no significant changes in these parameters were found when the animals were treated with the other diets. Our results suggest that an increase in the levels of dietary protein induces a rise in the intracellular levels of these enzymes. The possible role of this metabolic pathway in the kidneys under these nutritional conditions is also discussed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290080103
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