Publication Date:
1992-05-08
Description:
Controversy exists as to whether the interaction of a guanosine triphosphatase activating protein (GAP) with Ras proteins functions both to initiate and to terminate Ras-dependent signaling events or only to terminate them. GAP-C, a carboxyl-terminal fragment of GAP that is sufficient to stimulate GTPase activity, inhibited the stimulation of transcription produced by some oncoproteins (v-Src, polyoma middle T, wild-type Ras, and oncogenic Ras) but not that produced by v-Mos. Wild-type GAP did not affect transcription induced by oncogenic Ras but reversed the inhibitory effect of GAP-C on transcription induced by oncogenic Ras. These results indicate that GAP is a negative regulator of wild-type Ras and elicits a downstream signal by interacting with Ras-GTP (guanosine triphosphate).〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Schweighoffer, F -- Barlat, I -- Chevallier-Multon, M C -- Tocque, B -- New York, N.Y. -- Science. 1992 May 8;256(5058):825-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Rhone-Poulenc Rorer, Centre de Recherche de Vitry-Alfortville, Vitry Sur Seine.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1317056" target="_blank"〉PubMed〈/a〉
Keywords:
3T3 Cells
;
Animals
;
Antigens, Polyomavirus Transforming/genetics
;
CHO Cells
;
*Cell Cycle Proteins
;
*Cell Transformation, Neoplastic
;
Chloramphenicol O-Acetyltransferase/genetics/metabolism
;
Cricetinae
;
*Enhancer Elements, Genetic
;
Fungal Proteins/genetics/metabolism
;
GTPase-Activating Proteins
;
*Genes, ras
;
Humans
;
Mice
;
Oncogene Proteins v-mos
;
Oncogenes
;
Polyomavirus/*genetics
;
Promoter Regions, Genetic
;
Protein-Tyrosine Kinases/genetics
;
Proteins/*metabolism
;
Retroviridae Proteins, Oncogenic/genetics
;
Signal Transduction
;
Simian virus 40/genetics
;
Transcription, Genetic
;
*Transcriptional Activation
;
Transfection
;
ras GTPase-Activating Proteins
;
*ras-GRF1
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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