ISSN:
1432-2048
Keywords:
Cellular recognition Chlamydomonas (gametes)
;
Lipid turnover
;
Phosphoinositol lipid
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract In Chlamydomonas eugametos gametes, phosphatidylinositol 4-phosphate (PtdInsP) and phosphatidylinositol 4,5-bisphosphate (PtdInsP2) comprised 0.4 and 0.3% of the whole-cell phospholipids. They were concentrated in the plasma membrane around the cell body and were present in low concentrations in the flagellar membrane. When gametes were fed 32PO 4 - , the label was rapidly incorporated into PtdInsP and PtdInsP2 and only slowly incorporated into structural lipids such as phosphatidylethanolamine and phosphatidylglycerol. Similarly, when a pulse of 32PO 4 - was chased with PO 4 - , the label was rapidly lost from the polyphosphoinositol lipids but not from the structural lipids. The major fatty acids in the polyphosphoinositides were C-22 carbon polyenoic acids (70%). The significance of these results in relationship to intracellular signalling via inositol phosphates and Ca2+ is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00197945
Permalink
