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  • Immunocytochemistry  (32)
  • biological control  (28)
  • Calcium
  • Springer  (78)
  • 1990-1994  (78)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 64 (1992), S. 73-85 
    ISSN: 1570-7458
    Keywords: Trichogramma nubilale ; Ostrinia nubilalis ; inundative release ; sweet corn ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We conducted inundative release experiments withTrichogramma nubilale (Hymenoptera: Trichogrammatidae) to suppressOstrinia nubilalis (Lepidoptera: Pyralidae) in sweet corn (Zea mays): two experiment duringO. nubilalis first generation and three experiments during second generation. Five measurements of ear and stalk damage were used to assess.O. nubilalis control in treated and untreated plots within each experimental field. In one experiment during second generation, naturalO. nubilalis populations were sufficiently high to demonstrate that the parasitoids (three releases totaling 4.4 million parasitoids per ha) parasitized an estimated 57.4% of the placedO. nubilalis egg masses and reduced the mean number ofO. nubilalis larvae per ear by 97.4% the number of tunnels per stalk by 92.9%, and the number of larvae per stalk by 94.3% in the release plot. Ear damage in this experiment was suppressed to meet acceptable standards for use in cut-corn commercial processing. Larval mortality was apparently density independent, which implies that density-dependent larval loss would not compensate for egg parasitism byT. nubilale.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 125 (1990), S. 233-238 
    ISSN: 1573-5036
    Keywords: biological control ; bioluminescence ; Enterobacter cloacae ; lettuce ; Pythium ultimum ; rhizosphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plasmid pUCD607 was mobilized into the biocontrol agent Enterobacter cloacae strain E6 by conjugation and the resultant strain, E6(pUCD607), was bioluminescent. Biocontrol of Pythium ultimum by E6(pUCD607) was similar to that of the parent strain, E6. The location of E6(pUCD607) in the soil and in the rhizosphere of lettuce was readily determined by pressing agar medium against plant roots in a root box, allowing the bacteria to grow overnight on the medium, and detecting the presence of bioluminescence by autophotography. There was a positive, linear correlation between population sizes determined by dilution plating and the quantity of light emitted due to bioluminescence. However, both the intercept and slope of this line varied among experiments possibly due to the differing physiological states of cells recovered from soils. The amount of light emitted by the bioluminescent strain E6(pUCD607) was not quantitative. This technique is useful for qualitative determinations of populations and for photographically locating bacteria.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Entomologia experimentalis et applicata 64 (1992), S. 239-246 
    ISSN: 1570-7458
    Keywords: Diuraphis noxia ; Diaeretiella rapae ; Agropyron trachycaulum ; host plant resistance ; tritrophic interactions ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Interactions among three trophic levels of resistant and susceptible slenderwheat grasses, Elymus trachycaulum (Link) Goule ex Shinners ex. H.F. Lewis, Russian wheat aphid, Diuraphis noxia (Mordvilko), and a hymenopterous parasitoid were studied in the laboratory and greenhouse. These relationships were compared with a commercial susceptible wheat Triticum aestivum L. variety. Aphids reared on the resistant entries showed significantly lower weights and numbers. Significant reduction of parasitoid mummy weight and adult size was positively correlated with the effects on the aphids. Resistant entries also induced a longer prereproductive period for both the aphids and parasitoids. Numbers of aphids and aphid damage were significantly modified by the addition of parasitoids. Parasitism was higher on plants that did not have leaf rolling. These findings may indicate that antibiosis resistance studied here is not the most desirable because it decreases natural enemy vitality.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of comparative physiology 175 (1994), S. 415-423 
    ISSN: 1432-1351
    Keywords: Aplysia ; Calcium ; Circadian ; Light ; Serotonin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The eye of the marine mollusk Aplysia californica contains an oscillator that drives a circadian rhythm of spontaneous compound action potentials in the optic nerve. Both light and serotonin are known to influence the phase of this ocular rhythm. The aim of the present study was to evaluate the role of extracellular calcium in both light and serotonin-mediated phase shifts. Low calcium treatments were found to cause phase shifts which resembled those produced by the transmitter serotonin. However, unlike serotonin, low calcium neither increased ocular cAMP levels nor could these phase shifts be prevented by increasing extracellular potassium concentration. Low calcium-induced phase shifts were prevented by the simultaneous application of the translational inhibitor anisomycin and low calcium treatment resulted in changes in [35S]methionine incorporation into several proteins as measured by a two-dimensional electrophoresis gel analysis. Finally, light treatments failed to produce phase shifts in the presence of low calcium or the calcium channel antagonist nickel chloride. These results are consistent with a model in which serotonin phase shifts the ocular pacemaker by decreasing a transmembrane calcium flux through membrane hyperpolarization while light-induced phase shifts are mediated by an increase in calcium flux.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 15 (1993), S. 153-159 
    ISSN: 1432-0789
    Keywords: Calcium ; Maize ; Nitrogen ; Brazilian Amazon ; Cation leaching ; Canavalia ensiformes ; Mucuna aterrima
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This work investigated the effectsof amendments of fertilizer N and lime on subsoil acidity and maize rooting depth in an acid soil of the central Amazon basin. A split-plot designed field experiment was conducted on a clayey Oxisol (Typic Acrudox) during a 16-month period. Main plots received 0 or 4 Mt ha-1 of lime. Subplots were four crop sequences: (1) Maize-green manure (Canavalia ensiformes); (2) maize-green manure (Mucuna aterrima); (3) maize-bare fallow, with the maize receiving 300 kg ha-1 of urea-N; and (4) bare fallow, with an application of 300 kg ha-1 of urea-N at the same time as sequence 3. Plots were periodically sampled to 1.2 m. The experimental site received 4265 mm of precipitation during 16 months; approximately 60%–90% of this rain percolated through the profile. Substantial amounts of Ca were leached from the 0–30 cm horizon during the experimental period, but only limited amounts accumulated in the subsoil. Base saturation below 45 cm was less than 50% at the end of the experiment regardless of lime treatment. Roots of maize were concentrated in the 0–30 cm layers in limed plots and the 0–20 cm layers in unlimed plots. In all treatments less than 5% of the roots was found below 50 cm. An acidity balance indicated that considerable acidity was leached below the plow layer and out of the profile.
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  • 6
    ISSN: 1432-0878
    Keywords: Sodium influx-stimulating peptide, mollusc ; Neuroendocrine cells, mollusc ; Light yellow cells ; Yellow cells ; In situ hybridization ; Immunocytochemistry ; Osmoregulation ; Lymnaea stagnalis (Mollusca)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Neuroendocrine light yellow cells of the pond snail Lymnaea stagnalis express a neuropeptide gene encoding three different peptides. The morphology of the cell system has been studied by in situ hybridization, using two synthetic oligonucleotides encoding parts of light yellow cell peptides I and III, and by immunocytochemistry with antisera to synthetic light yellow cell peptide II and to two fragments of light yellow cell peptide I. One large cluster of light yellow cells was observed in the ventro-lateral protrusion of the right parietal ganglion, smaller clusters lying in the posterior dorsal part of this ganglion and in the visceral ganglion. The cells had an extended central neurohaemal area. Immunopositive axons projected into all nerves of the ganglia of the visceral complex, into the superior cervical and the nuchal nerves, and into the connective tissue surrounding the central nervous system. Axon tracts ramified between the muscle cells of the walls of the anterior aorta and of smaller blood vessels. Peripheral innervation by the light yellow cell system was only found in muscular tissue of the ureter papilla. The antisera to the two peptide fragments of light yellow cell peptide I not only stained the light yellow cells, but also the identified yellow cells, which have previously been shown to produce the sodium influx-stimulating neuropeptide. The latter cells were negative to the in situ hybridization probes and antisera specific to the light yellow cell system. It is therefore unlikely that the yellow cells express the light yellow cell neuropeptide gene. Nevertheless, the cells contain a neuropeptide sharing antigenic determinants with light yellow cell peptide I. Our observations support the hypothesis that light yellow cells are involved in maintaining the shape of the animal via the regulation of ion- and waterbalance processes and blood pressure.
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  • 7
    ISSN: 1432-0878
    Keywords: Neuropeptides (pancreatic polypeptide, peptide YY, neuropeptide Y) ; Immunocytochemistry ; Confocal scanning laser microscopy ; Schistosoma mansoni (Scolecida, Trematoda)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The presence and distribution of neuropeptides belonging to the pancreatic polypeptide family have been demonstrated by an indirect immunofluorescence technique in the nervous systems of adult male and female Schistosoma mansoni. Seven antisera of differing regional specificity to pancreatic polypeptide (PP), peptide YY (PYY) and neuropeptide Y (NPY) were employed on both whole-mount and cryostat-sectioned material. Positive immunoreactivity (IR) was obtained with all antisera except an N-terminally-directed antiserum to NPY. In the CNS, immunoreactivity was restricted to cell bodies and nerve fibres in the anterior ganglia, central commissure and dorsal and ventral nerve cords of both sexes, whereas, in the PNS, positive-IR was present in the plexuses innervating the subtegumental musculature and the oral and ventral suckers. Intense immunoreactivity was observed in a plexus of nerve fibres and cell bodies in the lining of the gynaecophoric canal and in fine nerve fibres innervating the dorsal tubercles of the male. In contrast, in the female, strong immunoreactivity was evident in nerve plexuses innervating the lining of the ovovitelline duct and in the wall of the ootype, but most notably in a cluster of cells in the region of Mehlis' gland. Results suggest that molecules with C-terminal homology to the PP-family are present in S. mansoni. These peptides would appear to be important regulatory molecules in the parasite's nervous system and may play a role in the control of egg production.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    BioControl 36 (1991), S. 105-113 
    ISSN: 1573-8248
    Keywords: Insecta ; Richogrammatidae ; Pyralidae ; Ostrinia nubilalis ; biological control ; inundative release ; Insecte ; Trichogrammatidae ; Pyralidae ; Ostrinia nubilalis ; lutte biologique ; lâcher inondatif
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Nous évaluons combien deTrichogramma nubilale devraient être lâchés en une seule localité pour lutter contreOstrinia nubilalis dans le maïs doux. Six parcelles de 8,6×16 m recevaient de 18,4 à 2 090 femelles deT. nubilale/unité de surface, quand les pieds étaient au milieu du stade du dernier verticille, où l'unité de surface, est la surface du plant/m2. Pour évaluer le contrôle potentiel de nos lâchers, nous exposons les ooplaques d'O. nubilalis élevés au laboratoire aux parasitoïdes lâchés en quatre fois après le lâcher. Quand une ooplaque était parasitée parT. nubilale, 75,7% des œufs de l'ooplaque étaient parasités. Nous développons des équations pour estimer le pourcentage d'ooplaques qu'une seule femelle s'attendait à parasiter en une journée (efficacité du parasitisme) et les taux de disparition de femelle (mort et dispersion) si tous deux étaient constants durant notre expérience. Le taux exponentiel de disparition était −0,52±0,03 jour−1, ce qui impliquait que 40% des femelles restantes disparaissaient par jour. L'efficacité du parasitisme était 0,050% parasitisme/femelle/unité de surface/jour, ce qui impliquait que 351 000 femelles/ha seraient nécessaires pour atteindre 90% de parasitisme. En clair, pour queT. nubilale soit un agent de lutte biologique assuré de succès, il faut accroître l'efficacité du parasitisme et réduire les taux de disparition.
    Notes: Abstract We evaluated how manyTrichogramma nubilale should be released at a single location to controlOstrinia nubilalis in sweet corn. Six 8.6×16 m plots received 18.4 to 2 090 ΦΦT. nubilale/SAI when plants were in the mid to late whorl stage, where SAI, surface area index, is the plant surface area/m2. To evaluate the potential control by our releases, we exposed laboratory-rearedO. nubilalis egg masses to the released parasitoids at 4 times after the release. When an egg mass was parasitized byT. nubilale, 75.7% of the eggs in the egg mass were parasitized. We developed an equation to estimate the percent of egg masses that a single female was expected to parasitize in a day (efficiency of parasitism) and female disappearance (death and dispersal) rates, if both were constant during our experiment. The exponential disappearance rate was −0.52±0.03 day−1, which implied that 40% of the remaining ΦΦ disappeared per day. The efficiency of parasitism was 0.050% parasitism/Φ/SAI/day, which implied that at least 351,000 ΦΦ/ha would be needed to achieve 90% parasitism. Clearly, forT. nubilale to be a successful biological control agent, efficiency of parasitism must be increased and disappearance rates must be reduced.
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  • 9
    ISSN: 1573-8248
    Keywords: Staphylinidae ; Aleochara bilineata ; beneficial arthropods ; dispersal ; predation ; biological control ; arthropodes utiles ; dissémination ; prédation ; lutte biologique ; Staphylinidae ; Aleochara bilineata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Aleochara bilineata (Gyllenhal) [Coleoptera: Staphylinidae] est un prédateur et un parasite courants de la mouche des racines [Diptera: Anthomyiidae] dans les cultures commerciales et les jardins potagers. Pour évaluer l'activité de dissémination deA. bilineata dans les jardins potagers, on a lâché des coléoptères marqués à raison de 0 et de 1000 en 1987, et de 0, 250, 500, et 1000/jardin potager/semaine en 1988. Trois pour cent des coléoptères ainsi marqués ont été recapturés dans les jardins potagers d'origine. Les populations naturelles deA. bilineata sont très faibles dans tous les jardins potagers et on ne constate aucune augmentation décelable de 1987 à 1988. Les recaptures sont proportionnelles aux taux de lâchers. On n'observe aucune différence significative dans les taux de recapture entre les sexes. Les recaptures et les distributions non uniformes deA. bilineata marqués dans les jardins potagers révèlent qu'ils ont pu voler au moins à 5 km de distance dans les conditions urbaines et choisir certains jardins plutôt que d'autres comme emplacements propices à l'accouplement, la quête de nourriture et l'oviposition.
    Notes: Abstract Aleochara bilineata (Gyllenhal) [Coleoptera: Staphylinidae] is a common predator and endoparasite of root maggot [Diptera: Anthomyiidae] in both commercial crops and home gardens. To test dispersal activity ofA. bilineata in home gardens, marked beetles were released at rates of 0 and 1,000 in 1987 and 0, 250, 500, and 1,000/gardens/wk in 1988. Three percent of marked beetles were recaptured in release gardens. NaturalA. bilineata populations were very small in all gardens, and there was no detectable increase from 1987 to 1988. Recaptures were proportional to release rates. There was no significant difference in recapture rates between sexes. Recaptures and non-uniform distributions of markedA. bilineata in control gardens revealed that they were capable of flying at least 5 km under urban conditions, and of selecting particular gardens as suitable mating, foraging, and oviposition sites.
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  • 10
    ISSN: 1573-8248
    Keywords: biological control ; Pistia stratiotes ; Neohydronomus affinis ; water lettuce ; host specificity ; lutte biologique ; Pistia stratiotes ; Neohydronomus affinis ; laitue d'eau ; spécificité vis-à-vis de l'hôte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Neohydronomus affinis a été introduit en Australie comme agent de lutte biologique potentiel contre la laitue d'eau,Pistia stratiotes. Les études en quarantaine ont démontré que ce coléopètre était spécifique deP. stratiotes et il a été libéré dans les champs en 1982. En conséquence, il a été lâché comme agent de lutte contre cette mauvaise herbe dans plusieurs autres pays. En AustralieN. affinis réduit les infestations deP. stratiotes de 40% ou plus en l'espace de 12 à 18 mois. Les résultats sont discutés en fonction de son efficacité dans d'autres parties du monde. Nous concluons queN. affinis contrôle effectivementP. stratiotes dans les régions tropicales (à la latitude 22°), mais dans les régions plus froides son efficacité fluctue avec les conditions saisonnières.
    Notes: Abstract Neohydronomus affinis was imported into Australia as a potential biological control agent for the floating aquatic weedPistia stratiotes. Quarantine studies demonstrated that this weevil was host specific toP. stratiotes and it was released into the field in 1982. Subsequently it was released as a control agent for this weed in several other countries. In AustraliaN. affinis reduced infestations ofP. stratiotes by 40% or more within 12–18 months. The results are discussed in relation to its effectiveness in other parts of the world. We concluded thatN. affinis effectively controlsP. stratiotes in tropical regions (to lat. 22°), but in cooler regions its effectiveness fluctuates with seasonal conditions.
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