ISSN:
1612-1112
Keywords:
Time Optimization
;
Column liquid chromatography
;
Microbore
;
Theophylline
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Algorithms for the time optimization of an HPLC separation are presented. Resolution, column performance, extra-column variance, capacity factor, and column length are incorporated into these algorithms. Extracolumn variance for a high-speed microbore system (1 mm i. d. column) was assessed using 2nd moment variance and a non-linear extrapolation of tubing length to zero. Extra-column variance, as a function of flowrate, was incorporated into the reduced operating curve of column performance. The use of empirical correction factors for column reproducibility and homogeneity are suggested. Cutting to an optimum length did not degrade column performance. These algorithms were applied to the optimization of a microbore assay for theophylline, in a simple xanthine mixture. Resulting total analysis times were reduced to 10seconds per sample, at a flowrate of 700μl/min.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02280603
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