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  • Acidophiles  (2)
  • nitrogen
  • Springer  (4)
  • Copernicus
  • 1990-1994  (4)
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  • Springer  (4)
  • Copernicus
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Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 153 (1990), S. 392-398 
    ISSN: 1432-072X
    Keywords: Thiobacillus acidophilus ; Acidophiles ; Maintenance ; Growth yields ; Organic acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Heterotrophic growth of the facultatively chemolithoautotrophic acidophile Thiobacillus acidophilus was studied in batch cultures and in carbon-limited chemostat cultures. The spectrum of carbon sources supporting heterotrophic growth in batch cultures was limited to a number of sugars and some other simple organic compounds. In addition to ammonium salts and urea, a number of amino acids could be used as nitrogen sources. Pyruvate served as a sole source of carbon and energy in chemostat cultures, but not in batch cultures. Apparently the low residual concentrations in the steady-state chemostat cultures prevented substrate inhibition that already was observed at 150 μM pyruvate. Molar growth yields of T. acidophilus in heterotrophic chemostat cultures were low. The Y max and maintenance coefficient of T. acidophilus grown under glucose limitation were 69 g biomass · mol−1 and 0.10 mmol · g−1 · h−1, respectively. Neither the Y max nor the maintenance coefficient of glucose-limited chemostat cultures changed when the culture pH was increased from 3.0 to 4.3. This indicates that in T. acidophilus the maintenance of a large pH gradient is not a major energy-requiring process. Significant activities of ribulose-1,5-bisphosphate carboxylase were retained during heterotrophic growth on a variety of carbon sources, even under conditions of substrate excess. Also thiosulphate- and tetrathionate-oxidising activities were expressed under heterotrophic growth conditions.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 154 (1990), S. 576-583 
    ISSN: 1432-072X
    Keywords: Thiobacillus acidophilus ; Acidophiles ; Mixotrophic growth ; Chemostat cultures ; Formate metabolism ; Formaldehyde metabolism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Although the facultatively autotrophic acidophile Thiobacillus acidophilus is unable to grow on formate and formaldehyde in batch cultures, cells from glucose-limited chemostat cultures exhibited substrate-dependent oxygen uptake with these C1-compounds. Oxidation of formate and formaldehyde was uncoupler-sensitive, suggesting that active transport was involved in the metabolism of these compounds. Formate- and formaldehyde-dependent oxygen uptake was strongly inhibited at substrate concentrations above 150 and 400 μM, respectively. However, autotrophic formate-limited chemostat cultures were obtained by carefully increasing the formate to glucose ratio in the reservoir medium of mixotrophic chemostat cultures. The molar growth yield on formate (Y=2.5 g ·mol-1 at a dilution rate of 0.05 h-1) and RuBPCase activities in cell-free extracts suggested that T. acidophilus employs the Calvin cycle for carbon assimilation during growth on formate. T. acidophilus was unable to utilize the C1-compounds methanol and methylamine. Formate-dependent oxygen uptake was expressed constitutively under a variety of growth conditions. Cell-free extracts contained both dye-linked and NAD-dependent formate dehydrogenase activities. NAD-dependent oxidation of formaldehyde required reduced glutathione. In addition, cell-free extracts contained a dye-linked formaldehyde dehydrogenase activity. Mixotrophic growth yields were higher than the sum of the heterotrophic and autotrophic yields. A quantitative analysis of the mixotrophic growth studies revealed that formaldehyde was a more effective energy source than formate.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biogeochemistry 12 (1991), S. 135-148 
    ISSN: 1573-515X
    Keywords: fens ; management ; nitrogen ; phosphorus ; productivity ; vegetation ; wetlands
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract A fertilization experiment was carried out in 3 mesotrophic fens to investigate whether plant growth in these systems is controlled by the availability of N, P or K. The fens are located in an area with high N inputs from precipitation. They are annually mown in the summer to prevent succession to woodland. Above-ground plant biomass increased significantly upon N fertilization in the two “mid”-succession fens studied. In the “late”-succession fen that had been mown for at least 60 years, however, plant biomass increased significantly upon P fertilization. The mowing regime depletes the P pool in the soil, while it keeps N inputs and outputs in balance. A long-term shift occurs from limitation of plant production by N toward limitation by P. Hence, mowing is a suitable management tool to conserve the mesothrophic character of the fens.
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  • 4
    ISSN: 1573-5036
    Keywords: bulk density ; epoxy resin impregnation ; image analysis ; macroporosity ; nitrogen ; stability ; strength ; structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil aggregate stability, organic matter content, pH, bulk density, strength, and macropore attributes were assessed in order to evaluate the influence of grass root growth in a field sward. The amount of grass grown was varied by varying the quantity of applied nitrogen fertilizer: following one year with a uniform application rate, nitrogen fertilizer was applied over the subsequent three years to a compact soil at zero (N0), moderate (N1) and high (N2) rates. Differences in herbage production were evident in the three years of the contrasting nitrogen treatments. An index of soil aggregate stability increased in response to the increased grass growth promoted by heavier applications of nitrogen, but both bulk density and vane shear strength were unchanged. Binary images of the soil solid and pore space showed that for each treatment the largest volume of macropores occurred close to the surface, particularly in N0 where there was more pore space than in either N1 or N2. Analysis of the pore structure attributes of the binary images revealed further differences between treatments, in particular, at 40–80 mm depth, the soil in treatment N0 had fewer and smaller pores, and greater distance between pores, than the soil in the N1 and N2 treatments. The larger macropore volume in N1 and N2 constituted a major portion of the air-filled porosity when the soil was relatively wet. It was concluded that the root growth in the intensively cropped grassland was conducive to maintenance of a relatively stable and porous soil structure. An attendant increase in soil acidity close to the soil surface was a disadvantage of the larger nitrogen inputs.
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