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  • Articles  (2)
  • Genetic linkage  (2)
  • Springer  (2)
  • American Society of Hematology
  • 1990-1994  (2)
Collection
  • Articles  (2)
Publisher
  • Springer  (2)
  • American Society of Hematology
Years
  • 1990-1994  (2)
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 895-899 
    ISSN: 1432-2242
    Keywords: Cercis siliquastrum ; SDS-PAGE ; Seed proteins ; Polymorphism ; Genetic linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The patterns of C. siliquastrum seed storage proteins (“cercins”) are described using SDS-polyacrylamide gel electrophoresis. The polypeptides detected had very different molecular weights (ranging from 168 to 34 KDa) which, together with their high homogeneity, produced a very good resolution of bands. These proteins could be ascribed to five different loci. The analysis of seed sets of individual trees indicated that the love tree is almost completely autogamous with less than 5% of outcrosses. Although this mode of reproduction seems to produce a decrease in heterozygote frequency among the seeds of the population analysed, the levels of variability detected were very high for an autogamous plant: all of the loci were polymorphic, with a mean heterozygosity of 0.327 and a polymorphic index of 0.412. Protein segregation revealed a strong genetic linkage between three cercin loci (a, c and d) while the other two are independent.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 85 (1992), S. 423-434 
    ISSN: 1432-2242
    Keywords: Genetic models ; RFLP ; Additive and dominance effects ; Genetic linkage ; Genetic simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The decision of whether or not to use QTLassociated markers in breeding programs needs further information about the magnitude of the additive and dominance effects that can be estimated. The objectives of this paper are (1) to apply some of the Moreno-Gonzalez (1993) genetic models to backcross simulation data generated by the Monte Carlo method, and (2) to get simulation information about the number of testing progenies and mapping density in relation to the magnitude of gene effect estimates. Results of the Monte Carlo study show that the stepwise regression analysis was able to detect relatively small additive and dominance effects when the QTL are independently segregating. When testing selfed families derived from backcross individuals, dominance effects had a larger error standard deviation and were estimated at a lower frequency. Linked QTL require a higher marker mapping density on the genome and a larger number of progenies to detect small genetic effects. Reduction of the environmental error variance by evaluating selfed backcross families in replicate experiments increased the power of the test. Expressions of the number of progenies for detecting significant additive effects were developed for some genetic situations. The ratio of the within-backcross genetic variance to the square of a gene effect estimate is a function of the number of progenies, the heritability of the trait, the marker map density and the portion of the genetic variance explained by the model. Different values (from 0 to 1) assigned to ρ (relative position of the QTL in the marker segment) did not cause a large shift in the residual mean square of the model.
    Type of Medium: Electronic Resource
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