ALBERT

Notes: Ichthyophthirius multifiliis, a parasitic ciliate of freshwater fishes, was found to have surface antigens (Ag) which elieited immobilizing antibodies (Ab) when injected into rabbits. An effort was made to purify and characterize these Ag (referred to as immobilization Ag) because of their potential role in protective immunity in fishes. Mice immunized with theront cilia were used for production of immobilizing monoclonal antibodies (MAb). Hybridomas were screened by indirect immunofluorescent light microscopy and immobilization of live parasites. Six hybridomas producing immobilizing MAb were cloned. Immobilizing MAb were used to affinity purify Ag solubilized with Triton X-114 and Na deoxycholate. Two membrane protein Ag of approximately 48 and 60 kDa were identified. Immobilizing MAb failed to react with these Ag on Western blots and, conversely, MAb that reacted with the Ag on Western blots did not immobilize live organisms. These results suggest that immobilization required native conformational epitopes which were altered by Western blotting procedures. Individual MAb reactive on Western blots recognized both the 48- and 60-kDa proteins indicating the presence of common epitopes. Affinity purified Ag elicited immobilizing antisera when injected into rabbits, mice, and channel catfish.

Notes: A field plot experiment was carried out on an established grassland sward from 1983–88 inclusive to examine the effects of time of application, chemical form of nitrogen (N) and cattle slurry dry matter (DM) content on yield and efficiency of N use. Four forms of fertilizer N (a semi-organic fertilizer, a combined 2.1:1 (w/w) semi-organic/calcium ammonium nitrate (CAN) fertilizer, CAN and urea, each supplying 300 kg N ha−1 year−1, were applied with or without unseparated or separated cattle slurry at 93 and 73 g kg−1 DM respectively, both supplying approximately 150 kg N ha−1 year−1. All fertilizers and slurries were applied in three equal dressings (February/March, May/June and July/August). The efficiency of use of fertilizer and slurry N was evaluated by measuring DM yield, N uptake and apparent recovery of N in herbage at all harvests during each growing season.Fertilizer N application significantly increased (P〈0.001) the mean yields of herbage at each harvest in all years. The form of fertilizer N had no significant effect (P〉0.05) on first harvest and total herbage yields, nor on N uptakes by herbage at the first harvest in any year. The performance of urea and of CAN was more variable at the second and third harvests relative to that of the semi-organic or combined 2.2:1 (w/w) semi-organic/CAN sources which had similar efficiencies of N use. Lower DM production was associated with reduced uptake of N. Values for mean overall apparent recovery of N ranged from 57.9 ± 2.67% for the semi-organic fertilizer to 50.2±3.05% for CAN.Unseparated cattle slurry and separated cattle slurry produced similar herbage yields and N responses that were lower and more variable than with fertilizer N. The overall mean apparent recovery of N from unseparated cattle slurry was 25.5 ± 5.03% compared to 5.0 ± 4.74% for separated cattle slurry. Efficiency of N use was highest with spring applications and least with mid-season applications. Recoveries ranged from −29% for separated cattle slurry applied in June 1984 to 56% for unseparated and separated cattle slurry applied in February 1988 and June 1987 respectively. No interactions were recorded between cattle slurry and fertilizer N in terms of DM production or N uptake by herbage.The results of this study support the use of a fertilizer N source, selected on a least-cost basis, in combination with slurry to promote spring herbage production. For subsequent production, N should be supplied in fertilizer form only. The use of urea is risky under low rainfall conditions. Mechanical separation did not improve the efficiency of use of slurry N.

Notes: Hydrogen gas was discovered within the steel casing above standing water in a percussion-drilled borehole on the Hanlord Site in south-central Washington state. In situ measurements of the borehole fluids indicated anoxic, low-Eh (〈-400 mV) conditions. Ground water sampled from adjacent wells in the same formation indicated that the ground water was oxygenated. H2 was generated during percussion drilling, due to the decomposition of borehole waters as a result of aqueous reactions with drilled sediment and steel from the drilling tools or casing. The generation of H2 within percussion-drilled boreholes that extend below the water table may be more common than previously realized. The ambient concentration of H2 produced during drilling was limited by microbial activity within the casing-resident fluids. H2 was generated abiotically in the laboratory, whereby sterilized borehole slurry samples produced 100 times more H2 than unsterilizcd samples. It appears that H2 is metabolized by microorganisms and concentrations might be significantly greater if not for microbial metabolism.

Notes: This paper presents a simple heuristic for computing the number of kanbans in a kanban system. The production systems under consideration have a multistage, uncapacitated, assembly tree structure, with every stage producing only one item at a time. The experimental results show that this method generates very good solutions. In more realistic problems where the master production schedule incorporates a smoothed demand, the solution found under the proposed method is identical to that of the linear programming approximation. This simple heuristic can be used in the real world to quickly determine the number of kanbans for daily just-in-time operations.

Notes: Abstract. The effect of growth temperatures on quantum yield (φ) was examined for leaves at different stages of development within the immature canopies of two crops of field grown maize (Zea mays cv. LG11) sown on 3 May and 20 June 1990. During the period of 23 to 49d after sowing, the crop sown on the 3 May experienced temperatures below 10°C on 19 occasions compared with only two for the crop sown on 20 June. A period of severe chilling at the end of May and the beginning of June was associated with a marked reduction in φ for all leaves in the early-sown crop. This chill-induced depression in φ was greater in recently emerged than more mature leaves in the canopy and was found to be accompanied by modifications in the polypeptide profiles of thylakoids isolated from the leaves. During the chilling period, decreases in some polypeptides, notably in the range of 41–42 and 20kDa apparent molecular size, and increases of polypeptides of c. 15–16kDa were observed compared with leaves developing at warmer temperatures in July. The efficiency of converting intercepted radiation into dry matter (conversion efficiency) was 42% lower in the early- than late-sown crop, but no significant relationship between conversion efficiency and quantum yield was found in either treatment.

Notes: Abstract. The activities of five active-oxygen scavenging enzymes were compared for cold-lability and three were compared for chilling induction in two Zea genotypes of contrasting susceptibility to photoinhibition during chilling. Activities of superoxide dismutase (SOD, EC 1.15.1.1), ascorbate peroxidase (APX, EC 1.11.1.11), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), and glutathione reductase (GTR, EC 1.6.4.2) in leaf extracts from plants grown without chilling stress were assayed at 19°C and 5°C. Enzymes from the chilling-susceptible Z. Mays cv. LG11 had lower specific activities at 5°C than did enzymes from the chilling-tolerant Z. diploperennis, except for MDHAR where no significant differences were observed. The activities of SOD and APX from Z. diploperennis were double those of Z. mays at both assay temperatures. Monodehydroa-scrobate reductase and glutathione reductase activities in both species were reduced by 63–78% at a 5°C assay temperature. The dehydroascorbate reductase (DHAR) showed the greatest low-temperature lability losing 96% (Z. diploperennis) and 100% (Z. mays) of its activity at 5°C. To examine possible chilling-induced changes in levels of enzyme activity, plants of both Zea genotypes were transferred to growth chambers at 10°C at moderate light intensities. Glutathione reductase activity was found to increase within 24h in Z. diploperennis, but it decreased slightly in Z. mays. MDHAR activity decreased by 50% in Z. diploperennis but showed only a transient increase in activity in Z. mays.