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  • cDNA  (5)
  • permeability  (3)
  • Korea
  • thermal diffusivity
  • Springer  (10)
  • American Geophysical Union (AGU)
  • 1990-1994  (10)
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Keywords
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  • Springer  (10)
  • American Geophysical Union (AGU)
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor (1993)
    Springer
    Plant molecular biology 21 (1993), S. 39-45 
    Details
    ISSN: 1573-5028
    Keywords: actin-depolymerizing factor ; cDNA ; developmental regulation ; male gametophyte ; anther
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization expriments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039616
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  • 2
    Electronic Resource
    Electronic Resource
    A new model for impregnation mechanisms in different GF/PP commingled yarns (1994)
    Springer
    Applied composite materials 1 (1994), S. 351-371 
    Details
    ISSN: 1573-4897
    Keywords: commingled yarn ; impregnation ; consolidation ; thermoplastics ; processing ; permeability ; mechanical testing ; mathematical modelling ; compression moulding ; glass fibre bed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Impregnation mechanisms of different kinds of GF/PP commingled yarns have been studied. As the reinforcing fibres were always the same, a global description has been worked out. Two different mathematical approaches for fibre bed permeability (Kozeny-Carman and Gutowski) were compared. The constants of the applied mathematical models have to stay the same if the fibre reeinforcement and the fibre arrangement is the same. Neither the kind of matrix, nor the fibre volume content may change these constants. Differences in the degree of impregnation after the same process conditions can be only due to different sizes of fibre agglomerations, thus the initial distribution of reinforcing fibres and matrix. For an exact determination of impregnation times and conditions the exact distribution of fibres in the intermediate material and after processing has to be known. This distribution is determined by SEM microscopy and data given from the material supplier. The importance of different process parameters, such as temperature, pressure, processing time is weighted by determining the density and mechanical properties of the specimens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00568041
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  • 3
    Electronic Resource
    Electronic Resource
    A rice (Oryza sativa L.) cDNA encodes a protein sequence homologous to the eukaryotic ribosomal 5S RNA-binding protein (1993)
    Springer
    Plant molecular biology 23 (1993), S. 409-413 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; cloning ; rice ; L5 ; ribosomal 5 S RNA-binding protein ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A rice (Oryza sativa L.) cDNA clone coding for the cytoplasmic ribosomal protein L5, which associates with 5 S rRNA for ribosome assembly, was cloned and its nucleotide sequence was determined. The primary structure of rice L5, deduced from the nucleotide sequence, contains 294 amino acids and has intriguing features some of which are also conserved in other eucaryotic homologues. These include: four clusters of basic amino acids, one of which may serve as a nucleolar localization signal; three repeated amino acid sequences; the conservation of glycine residues. This protein was identified as the nuclear-encoded cytoplasmic ribosomal protein L5 of rice by sequence similarity to other eucaryotic ribosomal 5 S RNA-binding proteins of rat, chicken, Xenopus laevis, and Saccharomyces cerevisiae. Rice L5 shares 51 to 62% amino acid sequence identity with the homologues. A group of ribosomal proteins from archaebacteria including Methanococcus vanniellii L18 and Halobacterium cutirubrum L13, which are known to be associated with 5 S rRNA, also related to rice L5 and the other eucaryotic counterparts, suggesting an evolutionary relationship in these ribosomal 5 S RNA-binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00029016
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  • 4
    Electronic Resource
    Electronic Resource
    A nuclear gene with many introns encoding ammonium-inducible chloroplastic NADP-specific glutamate dehydrogenase(s) inChlorella sorokiniana (1991)
    Springer
    Plant molecular biology 17 (1991), S. 1023-1044 
    Details
    ISSN: 1573-5028
    Keywords: cDNA ; Chlorella sorokiniana ; gene sequence ; glutamate dehydrogenase ; introns ; NADP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chlorella sorokiniana possesses ammonium-inducible, chloroplastic, NADP-specific glutamate dehydrogenase (NADP-GDH) homo- or heterohexamers composed of α- and/or β-subunits which were previously shown to derive from precursor protein(s) of identical size. From the present studies, data are consistent with these two subunits being encoded by a single nuclear gene. The NADP-GDH gene is greater than 7 kb in length due to the presence of at least 21 introns, an unusually large number for a eukaryotic microorganism. The exons, identified by comparison with sequences of NADP-GDH cDNA clones, include a region which is highly conserved among NADP-GDH genes. This region in theC. sorokiniana gene is 77% and 73% identical to the corresponding regions in theEscherichia coli andNeurospora crassa NADP-GDH genes, respectively. Seventeen independent NADP-GDH cDNA clones were analyzed by restriction mapping and partial sequencing, and no differences were detected among them. The longest cDNA was fused in frame withlacZ in a Bluescript vector and was expressed inE. coli as NADP-GDH antigen. During a 240 min induction period, under conditions in which both types of subunits were synthesized, only a single (2.2 kb) NADP-GDH mRNA band was detected on northern blots using cDNA probes from the highly conserved and 3′-untranslated regions. Collectively, these results are consistent with a single mRNA encoding a precursor-protein which is differentially processed to yield either an α- or β-subunit.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037142
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  • 5
    Electronic Resource
    Electronic Resource
    Isolation and characterization of two β-tubulin cDNA clones from rice (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1975-1979 
    Details
    ISSN: 1573-5028
    Keywords: β-tubulin ; cDNA ; rice ; monocot ; gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNA clones encoding two different β-tubulins, RTUB-1 and RTUB-2, were isolated from a rice cDNA library and their nucleotide sequences were analyzed. The deduced amino acid sequences showed amino acid sequence identity between 92% and 97% with other plant β-tubulins. Southern blot analysis using gene-specific and coding-region probes suggested that β-tubulins in rice are encoded by multigene families. The two cDNA clones represent two subfamilies of rice tubulins. RTUB-1 and RTUB-2, consisting of 3 to 4 genes and a single gene, respectively. The transcript levels of RTUB-1 and RTUB-2 genes were higher in actively elongating tissues such as etiolated shoot tissues and light-grown root tissues of four-day old seedlings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019507
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  • 6
    Electronic Resource
    Electronic Resource
    Cloning of a cDNA encoding 1-aminocyclopropane-1-carboxylate synthase and expression of its mRNA in ripening apple fruit (1991)
    Springer
    Planta 185 (1991), S. 38-45 
    Details
    ISSN: 1432-2048
    Keywords: 1-Amninocyclopropane-1-carboxylate synthase ; cDNA ; Ethylene synthesis ; Fruit ripening ; Gene expression ; Malus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 1-Aminocyclopropane-1-carboxylate (ACC) synthase (EC 4.4.1.14) purified from apple (Malus sylvestris Mill.) fruit was subjected to trypsin digestion. Following separation by reversed-phase high-pressure liquid chromatography, ten tryptic peptides were sequenced. Based on the sequences of three tryptic peptides, three sets of mixed oligonucleotide probes were synthesized and used to screen a plasmid cDNA library prepared from poly(A)+ RNA of ripe apple fruit. A 1.5-kb (kilobase) cDNA clone which hybridized to all three probes were isolated. The clone contained an open reading frame of 1214 base pairs (bp) encoding a sequence of 404 amino acids. While the polyadenine tail at the 3′-end was intact, it lacked a portion of sequence at the 5′-end. Using the RNA-based polymerase chain reaction, an additional sequence of 148 bp was obtained at the 5′-end. Thus, 1362 bp were sequenced and they encode 454 amino acids. The deduced amino-acid sequence contained peptide sequences corresponding to all ten tryptic fragments, confirming the identity of the cDNA clone. Comparison of the deduced amino-acid sequence between ACC synthase from apple fruit and those from tomato (Lycopersicon esculentum Mill.) and winter squash (Cucurbita maxima Duch.) fruits demonstrated the presence of seven highly conserved regions, including the previously identified region for the active site. The size of the translation product of ACC-synthase mRNA was similar to that of the mature protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), indicating that apple ACC-synthase undergoes only minor, if any, post-translational proteolytic processing. Analysis of ACC-synthase mRNA by in-vitro translation-immunoprecipitation, and by Northern blotting indicates that the ACC-synthase mRNA was undetectable in unripe fruit, but was accumulated massively during the ripening proccess. These data demonstrate that the expression of the ACC-synthase gene is developmentally regulated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00194512
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  • 7
    Electronic Resource
    Electronic Resource
    Studies on the mechanisms of active ion fluxes across alveolar epithelial cell monolayers (1992)
    Springer
    Methods in cell science 14 (1992), S. 187-193 
    Details
    ISSN: 1573-0603
    Keywords: transport ; epithelium ; edema ; beta-agonist ; barrier ; permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate the cell physiologic and biological properties of the alveolar epithelium, we studied rat alveolar epithelial cell monolayers grown on permeable supports in primary culture. Type II alveolar epithelial cells were disaggregated using elastase, and partially purified on a discontinuous metrizamide gradient. These isolated cells were plated onto tissue culture-treated Nuclepore membrane filters at 1.5×106 cells/cm2 and maintained in a humidified incubator (5% CO2 in air, 37° C). After 2 days in culture, the bathing media on both sides of the cell monolayers were changed to fresh culture medium, thus removing nonadherent cells (mostly leukocytes). These monolayers exhibit a high transmonolayer resistance (〉2000 Ω-cm2) and actively transport ions. Radionuclide flux studies indicate that Na+ is the predominant ionic species absorbed actively under baseline conditions, accounting for about 80% of the total active ion transport. Cl− seems to be passively transported across the epithelium. However, when the epithelium is exposed to a beta-agonist (terbutaline), active absorption of Na+ is increased and active absorption of Cl− occurs. Although it is clear that both active Na+ and Cl− transport are dependent on Na+/K+-ATPase activity, and that Na+ enters cells predominantly through channels, the specific mechanisms by which Cl− enters and exits the alveolar epithelial cells remain unclear. The stimulated reabsorption of Na+ and Cl− may be important in helping to remove excess fluid from alveolar air spaces in the lung.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01409010
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  • 8
    Electronic Resource
    Electronic Resource
    Photoacoustic determination of thermophysical properties of thin metallic plates using parameter estimation (1991)
    Springer
    International journal of thermophysics 12 (1991), S. 1063-1075 
    Details
    ISSN: 1572-9567
    Keywords: parameter estimation ; photoacoustic technique ; thermal diffusivity ; thermal effusivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The phase and the amplitude of the photoacoustic signal were measured as a function of chopping frequency for several kinds of widely used thin metallic plates (stainless steel 304, brass, aluminum, and copper) attached to plexiglass backing. The experimental data have been analyzed systematically by parameter estimation technique based on the two-layer model developed from Rosencwaig-Gersho (R-G) theory. Using this analysis, the values of thermal diffusivity and thermal effusivity of the materials have been determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00503519
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  • 9
    Electronic Resource
    Electronic Resource
    Estimation of thermophysical properties of a film coated on a substrate using pulsed transient analysis (1993)
    Springer
    International journal of thermophysics 14 (1993), S. 135-147 
    Details
    ISSN: 1572-9567
    Keywords: thermal diffusivity ; film ; effusivity ; parameter estimation ; pulsed transient analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The thermophysical properties (thermal diffusivity, effusivity) of a film coated on a substrate have been measured by a pulsed transient analysis. The experimental approach is to utilize the film surface temperature decay following a heating pulse from a Q-switched Nd:glass laser. The temperature decay was measured using a HgCdTe infrared detector. Following the collection of data, a nonlinear least-squares regression was performed to estimate the optimal values of three separate thermal parameters by fitting the data to the semiinfinite substrate model solution. The model was checked systematically by analysis of the sensitivity and correlation of the three parameters, and the thermal diffusivity and effusivity ratio of the film and substrate were obtained from the optimal values of the estimated parameters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00522667
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  • 10
    Electronic Resource
    Electronic Resource
    Transport of Thyrotropin Releasing Hormone in Rabbit Buccal Mucosa in Vitro (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 1113-1122 
    Details
    ISSN: 1573-904X
    Keywords: thyrotropin releasing hormone ; buccal mucosa ; metabolism ; permeability ; in vitro tissue integrity ; transport pathways ; rate-limiting barrier
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The transport of thyrotropin releasing hormone (TRH) in rabbit buccal mucosa in vitro has been investigated with respect to (a) rate and type of metabolism of TRH on mucosal and serosal sides of buccal mucosa, (b) mechanism of TRH transport including charge effect on its permeability, and (c) pathway and rate-limiting regions of TRH movement. In addition, the integrity of excised buccal mucosa has been evaluated for purposes of in vitro solute diffusion experiments using tissue ATP level data, transmission electron microscopy, and TRH transport kinetic data. The results indicate that excised rabbit buccal mucosa can be used for TRH diffusion studies for approximately 6 hr. In addition, TRH apparently traverses buccal mucosa by simple diffusion with a steady-state permeability of about 10−7 cm/sec, and this permeability is independent of pH. Moreover, the primary pathway appears to be via the intercellular space in the rate-limiting barrier, i.e., the upper 50 µm of the epithelium. Finally, TRH is degraded predominantly by deamidase activity, which is followed by, to a lesser degree, carboxypeptidase metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1015883217858
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