Publication Date:
1992-05-15
Description:
Although much is known about the bacterial genetics of early nodulation, little is known about the plant cell response. Alfalfa root hair cells were impaled with intracellular microelectrodes to measure a membrane potential depolarizing activity in Rhizobium meliloti cell-free filtrates, a plant response dependent on the bacterial nodulation genes. The depolarization was desensitized by repeated exposure to factors and was not observed in a representative nonlegume. A purified extracellular Nod factor, NodRm-IV(S), caused membrane potential depolarization at nanomolar concentrations. This rapid single-cell assay provides a tool for dissecting the mechanisms of host cell response in early nodulation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ehrhardt, D W -- Atkinson, E M -- Long, S R -- New York, N.Y. -- Science. 1992 May 15;256(5059):998-1000.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Stanford University, Stanford CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10744524" target="_blank"〉PubMed〈/a〉
Keywords:
Lipopolysaccharides/*pharmacology
;
Medicago sativa/cytology/*physiology
;
Membrane Potentials/drug effects
;
Plant Roots/cytology/drug effects/*physiology
;
Sinorhizobium meliloti/physiology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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