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  • Rhodococcus fascians  (3)
  • Scenedesmus incrassatulus  (3)
  • Springer  (6)
  • American Association for the Advancement of Science (AAAS)
  • De Gruyter
  • 1990-1994  (6)
Collection
Publisher
  • Springer  (6)
  • American Association for the Advancement of Science (AAAS)
  • De Gruyter
Years
  • 1990-1994  (6)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 487-493 
    ISSN: 1432-0614
    Keywords: Key words Limonin ; Debittering ; Immobilization ; Rhodococcus fascians ; Continuous Stirred Tank Reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Limonin can be effectively degraded by Rhodococcus fascians cells. These bacteria can be entrapped in κ-carrageenan, and used in a continuous stirred tank reactor to degrade limonin in a continuous process. The effects of temperature, limonin concentration, dilution rate, and aeration on the reactor behaviour have been tested, and the results correlated with changes in limonin conversion, substrate degradation rate, and free and immobilized biomass. Results showed that the immobilized cells were able to debitter limonin-containing media and the immobilized biomass was quite stable throughout the operational conditions tested. A population of free biomass was present in the reactor, the quantity of which was dependent on dilution rate. The immobilized bacteria increased its limonin-degrading capability when the substrate concentration was increased. The aeration was not strictly necessary for limonin degradation. Additionally, the immobilized cells were active and stable for more than 2 months of continuous operation, and were able to recover their limonin-degrading capability when used intermittently. Finally, none of the main components of a juice was noticeably altered during limonin degradation, so the reactor response was good enough to consider its application.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 487-493 
    ISSN: 1432-0614
    Keywords: Limonin ; Debittering ; Immobilization ; Rhodococcus fascians ; Continuous Stirred Tank ; Reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Limonin can be effectively degraded byRhodococcus fascians cells. These bacteria can be entraped in κ-carrageenan, and used in a continuous stirred tank reactor to degrade limonin in a continuous process. The effects of temperature limonin concentration, dilution rate, and aeration on the reactor behaviour have been tested, and the results correlated with changes in limonin conversion, substrate degradation rate, and free and immobilized biomass. Results showed that the immobilized cells were able to debitter limonin-containing media and the immobilized biomass was quite stable throughout the operational conditions tested. A population of free biomass was present in the reactor, the quantity of which was dependent on dilution rate. The immobilized bacteria increased its limonin-degrading capability when the substrate concentration was increased. The aeration was not strictly necessary for limonin degradation. Additionally, the immobilized cells were active and stable for more than 2 months of continuous operation, and were able to recover their limonin-degrading capability when used intermittently. Finally, none of the main components of a juice was noticeably altered during limonin degradation, so the reactor response was good enough to consider its application.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 487-493 
    ISSN: 1432-0614
    Keywords: Limonin ; Debittering ; Immobilization ; Rhodococcus fascians ; Continuous Stirred Tank ; Reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Limonin can be effectively degraded byRhodococcus fascians cells. These bacteria can be entraped in κ-carrageenan, and used in a continuous stirred tank reactor to degrade limonin in a continuous process. The effects of temperature limonin concentration, dilution rate, and aeration on the reactor behaviour have been tested, and the results correlated with changes in limonin conversion, substrate degradation rate, and free and immobilized biomass. Results showed that the immobilized cells were able to debitter limonin-containing media and the immobilized biomass was quite stable throughout the operational conditions tested. A population of free biomass was present in the reactor, the quantity of which was dependent on dilution rate. The immobilized bacteria increased its limonin-degrading capability when the substrate concentration was increased. The aeration was not strictly necessary for limonin degradation. Additionally, the immobilized cells were active and stable for more than 2 months of continuous operation, and were able to recover their limonin-degrading capability when used intermittently. Finally, none of the main components of a juice was noticeably altered during limonin degradation, so the reactor response was good enough to consider its application.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Journal of applied phycology 6 (1994), S. 423-425 
    ISSN: 1573-5176
    Keywords: Ankistrodesmus falcatus ; Scenedesmus incrassatulus ; Chlorophyceae ; mass production ; microalgae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A laboratory-scale system for mass culture of microalgae in 8-, 20- and 40-L polyethylene bags, was designed. Bags are 16.8 cm diameter and 52 cm (8-L bags), 112 cm (20-L) or 224 cm (40-L) length. The system was tested successfully with two freshwater microalgae,Ankistrodesmus falcatus andScenedesmus incrassatulus, cultured in Bold's Basal medium (prepared with either deionized or tap water). The procedure described is simple, reliable and practical, and enables a very cost-effective production of freshwater microalgae to satisfy any laboratory requirements, and when quantities demanded for special applications can not be met by the standard laboratory culture procedures.
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  • 5
    ISSN: 1573-5117
    Keywords: Daphnia magna ; Ankistrodesmus falcatus ; Scenedesmus incrassatulus ; Cladocera ; reproduction ; culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The freshwater microalgae Ankistrodesmus falcatus and Scenedesmus incrassatulus, at three concentrations (6, 12 and 18 mg l−1 t, dry weight), were tested as diets for Daphnia magna cultured in reconstituted hard water, at 19 ± 1 °C. Effects on survival, and reproductive characteristics were evaluated during a complete life-cycle. Test animals fed with the highest food concentration had the lowest survival, longevity, number of clutches, and average inter-brood times, but the total offspring was similar to that obtained with the lowest food concentration, for the same species of alga. Average longevity ranged from 40 to 85 days, with a minimum of 24 and a maximum of 119 days. The average inter-brood time ranged from 4.2 to 5.8 days, the highest values being observed at the lowest food concentration. The average total offspring was maximum for the intermediate concentrations of both algae. The maximum number of clutches ranged from 9 to 23, with the lowest values being observed at the highest food concentration. Even though both microalgae had similar effects on survival and reproduction, and the greatest differences observed were related to food concentration, it seems that S. incrassalulus is a comparatively better food for D. magna. The most suitable food level should be determined prior to carrying out chronic bioassays, and it is also an important factor in cultures for obtaining neonates for toxicological tests.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Journal of aquatic ecosystem stress and recovery 3 (1994), S. 247-253 
    ISSN: 1573-5141
    Keywords: Daphnia magna ; Scenedesmus incrassatulus ; microalgae ; chronic toxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Food concentration supplied during subchronic and chronic toxicity tests, is one of the most important factors that might influence the response of test organisms to toxicants. The green microalga Scenedesmus incrassatulus was used as food for the cladoceran Daphnia magna, in a chronic toxicity test with the toxicant sodium dodecyl sulphate (SDS). Test concentrations were 0.625, 1.25, and 2.5 mg l-1 of SDS, equivalent to 1/40, 1/20, and 1/10 of the average 48-h LC50 previously determined. Food concentrations were 9.5, 19, and 38 mg l-1 (dry wt.). Survival and reproduction were recorded, and the data were analyzed using a life-table approach. After 55 days, the main findings were as follows: 1. Average clutch size decreased as food concentration increased and there was a negative interaction between the toxicant and the algal concentration. 2. The Net Reproductive Rate (R 0) of the controls fed the lowest food concentration was approximately 10 times higher than the controls fed the highest food concentration. 3. R 0's for the treatments with SDS fed the lowest food concentration, were 2.5 to 5 times higher than those fed the highest food concentration. 4. Reproduction in controls and treatments with the same SDS concentrations, became similar as food level increased. According to these results, the food concentration to be supplied in chronic toxicity tests should be accurate, as an incorrect food concentration might negatively affect the survival and reproduction of test organisms, thus masking or confounding the effects of the toxicant being examined. A concentration around 10 mg l-1 (dry wt.) of S. incrassatulus seems to be appropriate for D. magna economic toxicity tests.
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