ISSN:
1573-5117
Keywords:
cyanophyte
;
carbon partitioning
;
lipid
;
carbohydrate
;
protein
;
centrifugation
;
autoclaving
;
L:D cycle
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Carbon partitioning techniques for the separation of physiologically important macromolecules (lipid, carbohydrate and protein) need to be efficient if they are to yield meaningful estimates of carbon flow in macromolecular synthesis, as measured by [14C] bicarbonate incorporation techniques. However, the efficiency of partitioning is rarely checked. Using existing methods, we found highly variable extraction efficiency of these macromolecules particularly of carbohydrate from cells grown under light:dark regimes. We report on a highly reproducible modification to existing methods which involves autoclaving with 5% trichloroacetic acid and centrifugation to separate carbohydrate and protein fractions. The presence of a structural non-extractable carbohydrate pool is reported.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00014051
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