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  • LIFE SCIENCES  (1)
  • autoclaving  (1)
  • 1990-1994  (2)
  • 1920-1924
  • 1
    Electronic Resource
    Electronic Resource
    The fractionation of cell components in Oscillatoria agardhii: an audit of procedures (1993)
    Springer
    Hydrobiologia 268 (1993), S. 163-168 
    Details
    ISSN: 1573-5117
    Keywords: cyanophyte ; carbon partitioning ; lipid ; carbohydrate ; protein ; centrifugation ; autoclaving ; L:D cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Carbon partitioning techniques for the separation of physiologically important macromolecules (lipid, carbohydrate and protein) need to be efficient if they are to yield meaningful estimates of carbon flow in macromolecular synthesis, as measured by [14C] bicarbonate incorporation techniques. However, the efficiency of partitioning is rarely checked. Using existing methods, we found highly variable extraction efficiency of these macromolecules particularly of carbohydrate from cells grown under light:dark regimes. We report on a highly reproducible modification to existing methods which involves autoclaving with 5% trichloroacetic acid and centrifugation to separate carbohydrate and protein fractions. The presence of a structural non-extractable carbohydrate pool is reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00014051
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    Paper (German National Licenses)
    Fulltext
  • 2
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    Unknown
    Portable Video/Digital Retinal Funduscope (1991)
    In:  Other Sources
    Details
    Publication Date: 2019-07-12
    Description: Lightweight, inexpensive electronic and photographic instrument developed for detection, monitoring, and objective quantification of ocular/systemic disease or physiological alterations of retina, blood vessels, or other structures in anterior and posterior chambers of eye. Operated with little training. Functions with human or animal subject seated, recumbent, inverted, or in almost any other orientation; and in hospital, laboratory, field, or other environment. Produces video images viewed directly and/or digitized for simultaneous or subsequent analysis. Also equipped to produce photographs and/or fitted with adaptors to produce stereoscopic or magnified images of skin, nose, ear, throat, or mouth to detect lesions or diseases.
    Keywords: LIFE SCIENCES
    Type: MSC-21675 , NASA Tech Briefs (ISSN 0145-319X); 15; 9; P. 115
    Format: text
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    NASA TECHNICAL REPORTS
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