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  • anther  (2)
  • 1990-1994  (2)
  • 1930-1934
  • 1
    Electronic Resource
    Electronic Resource
    The isolation and characterisation of the tapetum-specific Arabidopsis thaliana A9 gene (1992)
    Springer
    Plant molecular biology 19 (1992), S. 611-622 
    Details
    ISSN: 1573-5028
    Keywords: anther ; Arabidopsis thaliana ; Brassica napus ; tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Brassica napus cDNA clone A9 and the corresponding Arabidopsis thaliana gene have been sequenced. The B. napus cDNA and the A. thaliana gene encode proteins that are 73% identical and are predicted to be 10.3 kDa and 11.6 kDa in size respectively. Fusions of an RNase gene and the reporter gene β-glucuronidase to the A. thaliana A9 promoter demonstrated that in tobacco the A9 promoter is active solely in tapetal cells. Promoter activity is first detectable in anthers prior to sporogenous cell meiosis and ceases during microspore premitotic interphase. The deduced A9 protein sequence has a pattern of cysteine residues that is present in a superfamily of seed plant proteins which contains seed storage proteins and several protease and α-amylase inhibitors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026787
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The highly expressed tapetum-specific A9 gene is not required for male fertility in Brassica napus (1994)
    Springer
    Plant molecular biology 24 (1994), S. 97-104 
    Details
    ISSN: 1573-5028
    Keywords: anther ; antisense RNA ; Brassica napus ; male fertility ; tapetum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An antisense approach was used to attempt to determine the function of the highly abundant, tapetum-specific A9 transcript in microsporogenesis. A Brassica napus A9 cDNA clone was linked in sense and antisense orientations to the Arabidopsis thaliana A9 promoter and the resulting chimaeric genes introduced into B. napus. A high proportion of the offspring of B. napus antisense A9 plants had very low or undetectable levels of A9 mRNA. However, these plants set seed and had pollen of normal or near normal viability. Therefore, under the conditions studied, the A9 protein appears not to be essential for male fertility in B. napus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040577
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    Paper (German National Licenses)
    Fulltext
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