Publication Date:
1992-12-18
Description:
Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response to the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Devane, W A -- Hanus, L -- Breuer, A -- Pertwee, R G -- Stevenson, L A -- Griffin, G -- Gibson, D -- Mandelbaum, A -- Etinger, A -- Mechoulam, R -- DA 6481/DA/NIDA NIH HHS/ -- Wellcome Trust/United Kingdom -- New York, N.Y. -- Science. 1992 Dec 18;258(5090):1946-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Natural Products, Medical Faculty, Hebrew University, Jerusalem, Israel.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1470919" target="_blank"〉PubMed〈/a〉
Keywords:
Amides/chemistry/*isolation & purification/pharmacology
;
Animals
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*Arachidonic Acids
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Binding, Competitive
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Brain/*metabolism
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Brain Chemistry
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Cannabinoids/metabolism
;
Chromatography, Thin Layer
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Endocannabinoids
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Fatty Acids, Unsaturated/chemistry/*isolation & purification/pharmacology
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Gas Chromatography-Mass Spectrometry
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Kinetics
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Polyunsaturated Alkamides
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Receptors, Cannabinoid
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Receptors, Drug/*metabolism
;
Swine
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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