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  • Nitrate reductase  (3)
  • Zymomonas mobilis  (3)
  • 68A20  (2)
  • Springer  (8)
  • Blackwell Publishing Ltd
  • Nature Publishing Group
  • Oxford University Press
  • 1990-1994  (8)
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  • Springer  (8)
  • Blackwell Publishing Ltd
  • Nature Publishing Group
  • Oxford University Press
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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Computing 47 (1991), S. 137-151 
    ISSN: 1436-5057
    Schlagwort(e): AMS(MOS) 65N20 ; 65F10 ; 68A20 ; CR: G.1.3 ; G.1.8 ; Elliptic problems ; finite elements ; substructuring ; domain decomposition ; preconditioners ; parallel algorithms
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung In der vorliegenden Arbeit wird ein neuer Zugang zur Konstruktion von Vorkonditionierungsoperatoren auf der Basis von Gebietsdekompositionstechniken (DD Techniken) beschrieben. Anwendungen finden diese DD Vorkonditionierungen im Verfahren der konjugierten Gradienten zur iterativen Lösung von symmetrischen und positiv definiten Finiten-Elemente Gleichungen. Die DD Technik basiert auf einer Zerlegung des Gebietes Ω inp sich nicht überlappende Teilgebiete, die später denp Prozessoren eines MIMD Rechners zugeordnet sind. Die DD Vorkonditionierung enthält drei Blockmatrizen, die für ein konkretes Anwendungsproblem jeweils zu spezifizieren sind. Eine dieser Matrizen wird genutzt, um die Knotenbasis in eine näherungsweise diskret harmonische Basis zu transformieren. Die anderen beiden Matrizen können als Blockvorkonditionierungen für die in jedem Teilgebiet entstehenden Dirichlet-Probleme und für ein modifiziertes Schurkomplement auf den Knoten der Koppelränder zwischen den Teilgebieten interpretiert werden. Die relative spektrale Konditionszahl wird abgeschätzt. Eine direkte Verbindung der vorgeschlagenen DD Vorkonditionierung zu einer Additiven Schwarzschen Methode kann gezeigt werden. Im zweiten Teil dieser Artikelserie werden die Resultate dieser Arbeit auf ebene, symmetrische Randwertprobleme für partielle Differentialgleichungen zweiter Ordnung angewandt und die numerischen Resultate, die auf einem Transputer-Hypercube erzeugt wurden, diskutiert.
    Notizen: Abstract We present a new approach to the construction of Domain Decomposition (DD) preconditioners for the conjugate gradient method applied to the solution of symmetric and positive definite finite element equations. The DD technique is based on a non-overlapping decomposition of the domain Ω intop subdomains connected later with thep processors of a MIMD computer. The DD preconditioner derived contains three block matrices which must be specified for the specific problem considered. One of the matrices is used for the transformation of the nodal finite element basis into the approximate discrete harmonic basis. The other two matrices are block preconditioners for the Dirichlet problems arising on the subdomains and for a modified Schur complement defined over all nodes on the coupling boundaries between the subdomains. The relative spectral condition number is estimated. Relations to the additive Schwarz method are discussed. In the second part of this paper, we will apply the results of this paper to two-dimensional, symmetric, second-order, elliptic boundary value problems and present numerical results performed on a transputer-network.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Computing 47 (1991), S. 153-167 
    ISSN: 1436-5057
    Schlagwort(e): AMS (MOS) 65N20 ; 65F10 ; 68A20 ; CR: G. 1.3 ; G.1.8 ; Elliptic Problems ; finite elements ; substructuring ; domain decomposition ; preconditioners ; parallel algorithms ; hierarchical bases ; multigrid techniques
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Im ersten Teil dieser Artikelserie haben wir auf Basis von Gebietsdekompositionstechniken (DD Techniken) Vorkonditionierungsoperatoren konstruiert. Diese DD Vorkonditionierungen enthalten drei Blockmatrizen, die für spezifische Anwendungsfälle zu konkretisieren sind. In der vorliegenden Arbeit betrachten wir Finite-Elemente-Gleichungen, die bei der DD Diskretisierung von ebenen, symmetrischen, elliptischen Randwertproblemen für partielle Differentialgleichungen zweiter Ordnung entstehen. Zur Definition der oben genannten Blockmatrizen werden Mehrgitter-und hierarchische Techniken herangezogen. Die entstehenden DD-PCCCG Verfahren sind bezüglich des arithmetischen Aufwands asymptotisch fast optimal und bestens zur Parallelrechnung auf MIMD-Computern mit lokalem Speicher und Botschaftenaustausch geeignet. Die auf einem Transputer-Hypercube durchgeführten numerischen Experimente belegen nachhaltig die Effektivität der vorgeschlagenen DD Vorkonditionierungen.
    Notizen: Abstract In the first part of this article series, we had derived Domain Decomposition (DD) preconditioners containing three block matrices which must be specified for specific applications. In the present paper, we consider finite element equations arising from the DD discretization of plane, symmetric, 2nd-order, elliptic b.v.p.s and specify the matrices involved in the preconditioner via multigrid and hierarchical techniques. The resulting DD-PCCG methods are asymptotically almost optimal with respect to the operation count and well suited for parallel computations on MIMD computers with local memory and message passing. The numerical experiments performed on a transputer hypercube confirm the efficiency of the DD preconditioners proposed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 154 (1990), S. 536-543 
    ISSN: 1432-072X
    Schlagwort(e): Zymomonas mobilis ; Glucose dehydrogenase ; Pyrroloquinoline quinone ; Ubiquinone ; Electron transport chain ; TMPD oxidase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The interaction of the membrane-bound glucose dehydrogenase from the anaerobic but aerotolerant bacterium Zymomonas mobilis with components of the electron transport chain has been studied. Cytoplasmic membranes showed reduction of oxygen to water with the substrates glucose or NADH. The effects of the respiratory chain inhibitors piericidin, capsaicin, rotenone, antimycin, myxothiazol, HQNO, and stigmatellin on the oxygen comsumption rates in the presence of NADH or glucose as substrates indicated that a complete and in the most parts identical respiratory chain is participating in the glucose as well as in the NADH oxidation. Furthermore, the presence of coenzyme Q10 (ubiquinone 10) in Z. mobilis was demonstrated. Extraction from and reincorporation of the quinone into the membranes revealed that ubiquinone is essential for the respiratory activity with glucose and NADH. In addition, a membrane-associated tetramethyl-p-phenylene-diamine-oxidase activity could be detected in Z. mobilis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 159 (1993), S. 197-199 
    ISSN: 1432-072X
    Schlagwort(e): Zymomonas mobilis ; Pyruvate dehydrogenase multienzyme complex ; Anaerobic formation of acetyl-CoA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In the gram negative, obligately ethanologenic bacterium Zymomonas mobilis a pyruvate dehydrogenase complex was identified and the complex was enriched from cell extracts. This multienzyme complex is responsible for acetyl-CoA biosynthesis from pyruvate. No activities of related multienzyme complexes, 2-ketoglutarate dehydrogenase and branched chain keto acid dehydrogenase, could be detected.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 160 (1993), S. 74-79 
    ISSN: 1432-072X
    Schlagwort(e): Zymomonas mobilis ; Oxidative phosphorylation ; membrane vesicles ; ATP synthesis ; transmembrane pH gradient ; 31P-NMR ; Acetaldehyde ; Ethanol metabolism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The obligately fermentative aerotolerant bacterium Zymomonas mobilis was shown to possess oxidative phosphorylation activity. Increased intracellular ATP levels were observed in aerated starved cell suspension in the presence of ethanol or acetaldehyde. Ethanolconsuming Z. mobilis generated a transmembrane pH gradient. ATP synthesis in starved Z. mobilis cells could be induced by external medium acidification of 3.5–4.0 pH units. Membrane vesicles of Z. mobilis coupled ATP synthesis to NADH oxidation. ATP synthesis was sensitive to the protonophoric uncoupler CCCP both in starved cells and in membrane vesicles. The H+-ATPase inhibitor DCCD was shown to inhibit the NADH-coupled ATP synthesis in membrane vesicles. The physiological role of oxidative phosphorylation in this obligately fermentative bacterium is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    ISSN: 1617-4623
    Schlagwort(e): Nicotiana tabacum ; Nitrate reductase ; Retrotransposon ; Transcription
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The effects of Tntl retrotransposon insertion on nitrate reductase (NR) gene transcription have been analyzed in three NR-deficient insertional, mutants of Nicotiana tabacum. In the three mutants, named h9-Nia4, h9-Nia5 and h9-Nia6, Tnt1 was inserted into exon 3, exon 2 and exon 1 of the nia2 NR alloallelle, respectively. The mutants h9-Nia4 and h9-Nia6, which contained Tnt1 insertions that were oriented opposite to the direction of nia2 gene transcription, expressed chimaeric nia2-Tnt1 RNAs, respectively 12 kb and 10 kb long. The size observed in h9-Nia6 was close to the expected size for a full-length hybrid transcript starting and ending under the control of nia2 signals (about 9 kb). The larger transcript found in h9-Nia4 was shown to be due to a failure to splice the nia2 intron 2. The mutant h9-Nia5, which contained a Tntl insertion oriented in parallel with the direction of nia2 transcription expressed two truncated nia2-Tnt1 RNAs, 2 kb and 6.7 kb long. These transcripts arose from termination in the long terminal repeats (LTRs) of Tull. Since no full-length hybrid RNA was detected, we suggest that Tnt1 carries efficient termination signals, which are more efficiently recognized in the 3′ LTR than in the 5′ LTR.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    ISSN: 1617-4623
    Schlagwort(e): Nitrate reductase ; Reporter gene ; Nicotiana tabacum ; Nicotiana plumbaginifolia ; Transgenic plant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Reporter gene techniques and mutant analysis were used to identify the molecular basis of the regulation of the expression of nitrate reductase (NR) by nitrate and nitrate-, or ammonium-derived metabolites (N-metabolites), in the true diploïd species Nicotiana plumbaginifolia and in the amphidiploïd species Nicotiana tabacum. The N. plumbaginifolia mutant E23 results from the insertion of a Tnt1-like retrotransposon (Tnp2) in the first exon of the single-copy nia gene, which encodes nitrate reductase. One of the resulting transcripts ends in the 5′ LTR (long terminal repeat) sequence of this retrotransposon, and another one in the 3′ LTR. Nitrate and N-metabolites modulate the expression of these truncated transcripts, indicating that intron splicing and termination processes are not essential to these regulatory events. A GUS reporter sequence was transcriptionally linked to the promoter of the nia-1 gene of N. tabacum. This fusion was functional in transient expression assays done with protoplasts derived from mesophyll cells of N. tabacum. However none of the regulatory mechanisms known to affect steady-state levels of the nia-1 transcript were operative under these experimental conditions. Transgenic plants carrying either this fusion or translational fusions of GUS linked to the promoter of either the nia-1 or nia-2 gene of N. tabacum were obtained by Agrobacterium-mediated transfer. A low proportion of the transgenic plants (22 out of 105 independent transformants) expressed GUS activity although at a low level. Only 4 plants exhibited a detectable level of GUS mRNA. The concentration of this mRNA increased significantly in an NR-deficient background, indicating regulation by N-metabolites. Only 2 plants, however, showed regulation (induction) by nitrate. Attempts to use aux2 or nptII reporter sequences linked to either the nia-1 or nia-2 promoter as marker genes for the selection of regulatory mutants of the nitrate assimilation pathway were unsuccessful because of our inability to isolate transgenic plants in which these reporter genes were properly regulated by nitrate. The implications of these results are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1617-4623
    Schlagwort(e): Transposable element ; Nitrate reductase ; Nicotiana plumbaginifolia ; γ-Ray mutagenesis ; Nucleotide sequence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract By Northern blot analysis of nitrate reductase-deficient mutants of Nicotiana plumbaginifolia, we identified a mutant (mutant D65), obtained after γ-ray irradiation of protoplasts, which contained an insertion sequence in the nitrate reductase (NR) mRNA. This insertion sequence was localized by polymerase chain reaction (PCR) in the first exon of NR and was also shown to be present in the NR gene. The mutant gene contained a 565 by insertion sequence that exhibits the sequence characteristics of a transposable element, which was thus named dTnp1. The dTnp1 element has 14 by terminal inverted repeats and is flanked by an 8-bp target site duplication generated upon transposition. These inverted repeats have significant sequence homology with those of other transposable elements. Judging by its size and the absence of a long open reading frame, dTnp1 appears to represent a defective, although mobile, transposable element. The octamer motif TTTAGGCC was found several times in direct orientation near the 5′ and 3′ ends of dTnp1 together with a perfect palindrome located after the 5′ inverted repeat. Southern blot analysis using an internal probe of dTnp1 suggested that this element occurs as a single copy in the genome of N. plumbaginifolia. It is also present in N. tabacum, but absent in tomato or petunia. The dTnp1 element is therefore of potential use for gene tagging in Nicotiana species.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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