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  • Physical Chemistry  (7)
  • Cell & Developmental Biology  (4)
  • 1990-1994  (11)
  • 1965-1969
  • 1
    Electronic Resource
    Electronic Resource
    Trigeminal primary afferent projections to the spinal cord of the frog, Rana ridibunda (1993)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 217 (1993), S. 137-146 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The distribution in the spinal cord of the trigeminal primary projections in the frog Rana ridibunda was studied by means of the anterograde transport of horseradish peroxidase (HRP). Upon entering the medulla via the single trigeminal root, a conspicuous descending tract that reaches the cervical spinal cord segments is established. This projection arises in the ophthalmic (V1), maxillary (V2), and mandibular (V3) trigeminal nerve subdivisions. In the spinal cord, only a minor somatotopic arrangement of the trigeminal fibers was observed, with the fibers arising in V3 terminating somewhat more medially than those from V1 and V2. A dense projection to the medial aspect of the spinal cord, above the central canal, primarily involves V3. Each trigeminal branch sends projections at cervical levels to the contralateral dorsal field, and those from V2 are most abundant. Bilateral experiments with HRP application show convergence of primary trigeminal and spinal afferents within the dorsal field of the spinal cord.The pattern of arrangement of the trigeminal primary afferent fibers in the spinal cord of this frog largely resembles that of amniotes. However, the organization seems simpler and the slight somatotopic distribution of V1, V2, and V3 fibers is similar to the condition in other anamniotes. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052170203
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  • 2
    Electronic Resource
    Electronic Resource
    Regulation of the sarcoplasmic reticulum Ca2+-release channel requires intact annexin VI (1991)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 46 (1991), S. 86-93 
    Details
    ISSN: 0730-2312
    Keywords: Ca2+-dependent ; phospholipid-binding ; proteolysis ; purification ; repeats ; immunoreactivity ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Annexin VI has eight highly conserved repeated domains; all other annexins have four. Díaz-Muñoz et al. (J Biol Chem 265:15894, 1990) reported that annexin VI alters the gating properties of the ryanodine-sensitive Ca2+-release channel isolated from sarcoplasmic reticulum. To investigate the domain structure of rat annexin VI (67 kDa calcimedin) required for this channel regulation, various proteolytic digestions were performed. In each case, protease-resistant core polypeptides were produced. Annexin VI was digested with V8 protease and two core polypeptides were purified by Ca2+-dependent phospholipid binding followed by HPLC. The purified fragments were shown to be derived from the N- and C-terminal halves of annexin VI, and demonstrated differential immunoreactivity with monoclonal antibodies to rat annexin VI. While both core polypeptides retained their ability to bind phospholipids in a Ca2+-dependent manner, they did not regulate the sarcoplasmic reticulum Ca2+-release channel as did intact annexin VI.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240460113
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  • 3
    Electronic Resource
    Electronic Resource
    Eukaryotic-like protein serine/threonine kinases in Myxococcus xanthus, a developmental bacterium exhibiting social behavior (1993)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 51 (1993), S. 29-33 
    Details
    ISSN: 0730-2312
    Keywords: protein kinases ; myxobacteria ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Myxococcus xanthus, a gram-negative bacterium exhibits a spectacular life cycle and social behavior. Its developmental cycle and multicellular morphogenesis resemble those of eukaryotic slime molds such as Dictyostelium discoideum. On the basis of this resemblance, we explored the existence of eukaryotic-like protein serine/threonine kinases which are known to play important roles in signal transduction during development of D. discoideum. It was indeed found that M. xanthus contains a large family of protein serine/threonine kinases related to the eukaryotic enzymes. This is the first unambiguous demonstration of eukaryotic-like protein serine/threonine kinases in the prokaryotes. © 1993 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240510107
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  • 4
    Electronic Resource
    Electronic Resource
    Classification of the solubility behaviour of β-cyclodextrin in aqueous-CO-solvent mixtures (1993)
    Chichester : Wiley-Blackwell
    Journal of Physical Organic Chemistry 6 (1993), S. 651-659 
    Details
    ISSN: 0894-3230
    Keywords: Organic Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The solubility behaviour of β-cyclodextrin in aqueous mixtures was studied by determining the interactions between the co-solvent and water. Three clear classes of co-solvent are evident. In the first, including ethanol, tetrahydrofuran and acetone, there is formation of clathrate hydrates by the co-solvent. In these systems the solubilization is a maximum at the mole fraction corresponding to the minimum in the partial excess molar volume. The second class, including such solvents as dimethyl sulphoxide and pyridine, forms strong molecular complexes with water, and here a valley and a plateau region occur in the solubility values. In the third class, including formamide and urea, a steady increase in the solubility is observed. For these highly polar solvents no maxima are observed in intrinsic solvent properties such as the partial excess molar volume.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/poc.610061203
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  • 5
    Electronic Resource
    Electronic Resource
    Electrogenic Na-independent HCO3 transport in OK cells (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 153 (1992), S. 22-29 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have shown previously that OK cells recover from an acid load in a medium nominally CO2-free by extruding H via a Na/H exchanger and a passive H-conductive pathway. In this work, the regulation of cell pH (pHi) was studied after additon or withdrawal of CO2/HCO3 (5%CO2, 95 mM HCO3, pH = 8) using the fluoroprobe BCECF. In the presence of Na and amiloride to inhibit Na/H exchange, the recovery of pHi after CO2 entry and CO2 exit were found to depend in part on HCO3 entry and exit, respectively. Efflux of H per se also contributed to restoring pHi after CO2 addition, whereas H influx may have played a smaller role to normalize pHi after CO2 removal. DIDS, 0.5mM, significantly inhibited both recovery phases of pHi. Removal of Na failed to inhibit the recovery of pHi after CO2 addition and removal. Cl removal also failed to inhibit pHi recovery after CO2 removal. Cell depolarization in the presence of Na moderately stimulated the pHi recovery rate after CO2 addition whereas it markedly inhibited the normalization of pHi after CO2 removal. Cell depolarization in the absence of sodium had only a slight effect to increase pHi recovery after CO2 addition but markedly prevented the pHi recovery after CO2 removal. These results indicate that OK cells lack Na or Cl-dependent HCO3 transport systems. The OK cell possesses a novel stilbene-sensitive electrogenic HCO3 transport system that is involved in the regulation of cell pH. © 1992 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041530105
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of the side chain on the stability of Schiff-bases formed between pyridoxal 5′-phosphate and amino acids (1990)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 22 (1990), S. 905-914 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The stability of some Schiff-bases formed between PLP and different amino acids has been investigated in a wide range of pH. The kinetic constants of formation of these compounds and their hydrolysis rate constants have been determined. Results show that the α-position of the carboxyl group of amino acid plays an important role on the mechanism of water attack upon the C=N—bond. The absence of ionic groups in the surroundings of that bond must be an important factor of stability. Bulky hydrophobic substituents in the amino acid, near the amine part, protect the imine bond against hydrolysis.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550220903
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  • 7
    Electronic Resource
    Electronic Resource
    Schiff bases between pyridoxal 5′-phosphate and dodecylamine. Kinetic study (1992)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 24 (1992), S. 67-78 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We studied the formation and hydrolysis of the Schiff base of pyridoxal-5′ -phosphate (PLP) and n-dodecylamine (DOD) by kinetic methods and found the formation rate constants to be much larger than those reported for related systems. This can be ascribed to the occurrence of local concentration of charges in the vicinity of the carbinolamine, which stabilizes the corresponding transition state of the dehydration reaction. On the other hand, the hydrolysis rate constants of our system were considerably smaller as a result of the 12-atom hydrocarbon chain acting as a bulky hydrophobic rest protecting the imine bond from attack by water.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550240108
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  • 8
    Electronic Resource
    Electronic Resource
    The formation of the complex pentacyano(3-pyrazincarboxylate)ferrate(II) in various water-cosolvent mixtures (1990)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 22 (1990), S. 1017-1026 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: This article presents the kinetic of formation of pentacyano(3-pyrazincarboxylate)ferrate(II) from pyrazincarboxylate and pentacyanoaquoferrate(II) ions in various isodielectric water-cosolvent mixtures at 298 K. The rate law is in the form d[Fe(CN)5(3-pzCO2)4-]/dt = kf[Fe(CN)5H2O-3] [pzCO2-]. Plots of log(kf/dm3 mol-1 s-1) vs. Dm-1 (where Dm is the bulk dielectric constant of the medium) and log(kf/dm3 mol-1 s-1) vs. the Grunwald-Winstein parameter are nonlinear for some of the mixtures and to each mixture corresponds a different behavior in respect to the above parameters. The plots of log(kf/dm3 mol-1 s-1) vs. the mol fraction of water are straight lines over the entire composition range studied, except for the water-methanol mixture. It is evident that the solvation phenomenon plays a dominant role and that the rate of formation is mediated by the dual solvent vectors, the overall basicity and acidity of the solvent mixtures.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550221002
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  • 9
    Electronic Resource
    Electronic Resource
    Alkaline hydrolysis of cephaloridine: An 1H—NMR study. Temperature dependence of the rate constants (1993)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 25 (1993), S. 865-874 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A kinetic study on the basic hydrolysis of cephaloridine at pD= 10.5 was carried out by using the 1H—NMR technique. Epimerization at H7, a nucleophilic attack of hydroxyl ion on the β-lactam carbonyl group followed by the release of the pyridine molecule, and isomerization of the double bond at position 3 in the dihydrothiazine ring were the major reactions observed.Based on the results obtained, it should be emphasized that the presence of a pyridine group at 3′ results in a slightly increased formation constant for the exo methylene compound relative to other cephalosporins with different substituents at that position.The activation energy for the epimerization constant and the cleavage of the β-lactam ring at pD 10.5 was 21.2 kcal/mol. © 1993 John Wiley & Sons, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550251007
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  • 10
    Electronic Resource
    Electronic Resource
    Salt effects upon reactions of different charge type reactants: Peroxodisulphate Oxidations of Fe(CN)4(bpy)2-, cis-Fe(CN)2(bpy)2 and Fe(bpy)32+ and Iron(II) Oxidation by Co(NH3)5Cl2+ (1994)
    New York, NY : Wiley-Blackwell
    International Journal of Chemical Kinetics 26 (1994), S. 299-307 
    Details
    ISSN: 0538-8066
    Keywords: Chemistry ; Physical Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Salt effects on the oxidation of the iron(II) complexes Fe(CN)4(bpy)2-, cis-(CN)2(bpy)2 and Fe(bpy)32+ by S2O82- as well as on the reaction Fe2+ + Co(NH3)5Cl2+ have been studied in concentrated electrolyte solutions at 298.2 K. We have gone from anion-anion to cation-cation reaction with the intermediate cases of anion-neutral and cation-anion reactions. Results show that the main cause of the kinetic salt effects observed is the interaction between supporting electrolytes and the solvent. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/kin.550260208
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