Publication Date:
1992-04-10
Description:
The myoD family of DNA binding proteins has been implicated in the control of myogenesis in a variety of organisms. Searches for homologs in the nematode Caenorhabditis elegans yielded only one gene, designated hlh-1, expressed in body-wall muscle cells and their precursors. To assess the role of hlh-1 in C. elegans myogenesis, genetic deficiencies spanning the hlh-1 locus were isolated after gamma irradiation. Embryos homozygous for these deficiencies exhibited extensive body-wall muscle differentiation, including expression of several characteristic myofilament proteins and weak contracile behavior. Thus, zygotic hlh-1 expression was not required for body-wall muscle precursors to adopt muscle cell fates.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Chen, L -- Krause, M -- Draper, B -- Weintraub, H -- Fire, A -- R01 GM037706/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1992 Apr 10;256(5054):240-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Carnegie Institution of Washington, Baltimore, MD 21210.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1314423" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Caenorhabditis/embryology/genetics/*physiology
;
Cell Differentiation
;
*Chromosome Deletion
;
Chromosome Mapping
;
Crosses, Genetic
;
DNA/genetics/isolation & purification
;
DNA-Binding Proteins/*genetics
;
Embryo, Nonmammalian/cytology/physiology/radiation effects
;
Gamma Rays
;
Homozygote
;
Molecular Sequence Data
;
Multigene Family
;
Muscle Proteins/*genetics
;
Muscles/*embryology/physiology/radiation effects
;
MyoD Protein
;
Oligodeoxyribonucleotides
;
Polymerase Chain Reaction
;
Sequence Homology, Nucleic Acid
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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