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  • Springer  (128)
  • Public Library of Science (PLoS)
  • 1990-1994  (116)
  • 1965-1969  (12)
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  • 1
    Electronic Resource
    Electronic Resource
    Detection of brassinosteroids in pollen of Lolium perenne L. by immunocytochemistry (1993)
    Springer
    Planta 189 (1993), S. 91-100 
    Details
    ISSN: 1432-2048
    Keywords: Amyloplast (brassinosteroid storage) ; Brassinosteroid (immunolocalization) ; Lolium (pollen) ; Pollen (development, brassinosteroid)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Bioactive brassinosteroids have been localized in developing and mature pollen of anhydrously fixed rye-grass (Lolium perenne) by immunocytochemistry using polyclonal antibodies to castasterone generated in rabbits. Tricellular pollen fixed by freeze-substitution was also labelled in the starch granules. Study of the developmental sequence of the pollen through the microsporocyte, microspore, bicellular and tricellular stages showed that the brassinosteroids were increasingly sequestered in starch granules as the amyloplasts matured, supporting the view that these are storage organelles for these potent plant growth promoters. In bicellular pollen, heavy labelling was seen in the zone within 0.5 μm of the starch granule, where stromal tissue remains. Thus, the stroma may be the site of synthesis of these compounds. During aqueous fixation, the brassinosteroids leached from the starch granules of tricellular pollen, indicating that they would be quickly available after imbibition to influence the physiology of germinating pollen. The results from high-performance liquid chromatography of dansylaminophenylboronates from partially purified extracts of freshly dehisced tricellular pollen of rye-grass showed 25-methylcastasterone may be a minor component, together with two unknown peaks. No specific binding of brassinolide to any soluble proteins extracted from tricellular rye-grass pollen was observed using the antibodies in gel electrophoresis or enzyme-linked immunosorbent assays.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201349
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic variation in agronomically important species of Stylosanthes determined using random amplified polymorphic DNA markers (1993)
    Springer
    Theoretical and applied genetics 85 (1993), S. 882-888 
    Details
    ISSN: 1432-2242
    Keywords: Polymerase chain reaction ; Random amplified polymorphic DNA ; Stylosanthes spp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Random amplified polymorphic DNA (RAPD) markers were generated from 20 cultivars and accessions representing four agronomically important species of Stylosanthes, S. scabra, S. hamata, S. guianensis, and S. humilis. Approximately 200 fragments generated by 22 primers of arbitrary sequence were used to assess the level of DNA variation. Relatively low levels of polymorphism (0–16% of total bands in pairwise comparisons) were found within each species, while polymorphisms between the species were much higher (up to 46%). Very few polymorphisms (0–2%) were detected between the individuals of the same cultivar or accession. A phenogram of relationships among the species was constructed based on band sharing. Four main clusters corresponding to each species were readily distinguished on this phenogram. The allotetraploid species S. hamata and its putative diploid progenitor, S. humilis, were more similar to each other than to S. scabra and S. guianensis. No variation in RAPD markers was found between the two commercial S. hamata cvs ‘Verano’ and ‘Amiga’. Cultivar ‘Oxley’ in S. guianensis was considerably different from the other cultivars and accessions of this species. The phylogenetic distinctions obtained with RAPDs were in agreement with other studies from morphology, cytology, and enzyme electrophoresis. The low level of polymorphisms observed within each species suggested that interspecific crosses may be a better vehicle for the construction of RAPD linkage maps in Stylosanthes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225033
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  • 3
    Electronic Resource
    Electronic Resource
    Carbonatization and propylitic alteration of fragmental basaltic rocks, Quesnel River gold deposit, Central British Columbia (1990)
    Springer
    Mineralium deposita 25 (1990), S. S115 
    Details
    ISSN: 1432-1866
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract The Quesnel River gold deposit (1.2 million tonnes grading 5.22 g/t Au in three separate zones) occurs within Takla Group volcanic rocks of Upper Triassic age proximal to an alkalic stock. The deposit occurs in amphibole-augite phyric, fragmental, basaltic rocks. Alteration has produced an assemblage of epidote-chloritetremolite-calcite-quartz with lesser pyrite, chalcopyrite, pyrrhotite, sphalerite, marcasite, galena, arsenopyrite and gold. The West Zone comprises a tabular, conformable sulfide body underlain by bedded, variably altered fragmental basaltic rocks and overlain by siltstone and argillite. In the Main Zone, highest gold grades occur adjacent to a sharp discordant alteration front with barren, strongly carbonatized, pyritic basaltic lapilli-tuff. It is overlain by siltstone and argillite and bounded to the east and a depth by a west dipping reverse fault. To the west the auriferous, propylitically altered, rocks grade laterally into lower grade and barren basaltic rocks. Oxygen(δ18O = + 9 to + 15) and carbon (δ13O= -14 to −7) isotopic signatures of calcite from carbonate-altered and propylitically altered rocks are similar. However, sulfur isotopic values for pyrite are different, with gold-associated pyrite (δ34S = −7 to −3) distinct from pyrite in carbonate altered rocks with (δ34S = + 8 to + 13). The carbonization occurred before complete induration of the basaltic fragmental rocks, whereas propylitization and gold plus sulfide precipitation is clearly epigenetic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00205258
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  • 4
    Electronic Resource
    Electronic Resource
    Biochemical and energetic composition of bathyal echinoids and an asteroid, holothuroid and crinoid from the Bahamas (1990)
    Springer
    Marine biology 105 (1990), S. 175-183 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The biochemical and energetic composition of body components of ten species of bathyal echinoids, and an asteroid, a holothuroid and a stalked crinoid were determined from individuals sampled from a variety of deep-water sites near the Bahamas (north Caribbean Sea) in October 1988. When compared with other studies of echinoderms, no geographic- or depth-related differences in biochemical or energetic composition were found. Body-wall tissues were composed primarily of skeletal material (mineral ash), but were comparatively high in organic material in the echinothuriid echinoids, and the asteroid and holothuroid. Gut tissues and pyloric cecae had high levels of lipid and protein, indicating their potential role in nutrient storage. Body-wall tissues were generally low in energy, but were highest in the echinoidsAraeosoma belli (7.7 kJ g−1 dry wt) andSperosoma antillense (8.0 kJ g−1 dry wt), the asteroidOphidiaster alexandri (8.9 kJ g−1 dry wt), and the holothuroidEostichopus regalis (13.1 kJ g−1 dry wt). Energy levels of gut and pyloric cecal tissues were two to three times higher than those of body-wall tissues. Total somatic tissue energy values varied greatly among species, ranging from 1.5 kJ in the echinoidAspidodiadema jacobyi to 142.1 kJ inE. regalis. As the bathyal echinoderms examined in this study occur in great abundance, they represent a significant reservoir of organic and inorganic materials and energy in deep-water benthic systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01344284
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  • 5
    Electronic Resource
    Electronic Resource
    Ovoids in infinite incidence structures (1994)
    Springer
    Archiv der Mathematik 62 (1994), S. 189-192 
    Details
    ISSN: 1420-8938
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01198674
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  • 6
    Electronic Resource
    Electronic Resource
    Infrared-visible sum-frequency generation spectra of CO on Ni(100) (1990)
    Springer
    Applied physics 51 (1990), S. 221-225 
    Details
    ISSN: 1432-0630
    Keywords: 82.65 My ; 42.65 Ky
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract Infrared-visible sum-frequency generation (SFG) spectra of the stretching region of carbon monoxide adsorbed on Ni(100) are measured as a function of overlayer coverage and substrate temperature. The frequency and linewidth of the CO vibrational resonance in SFG have a similar dependence on coverage and temperature to that observed in linear absorption and emission spectroscopies. The lineshape of the SFG resonance, however, is influenced by the nonlinear response of the underlying metal surface. A simple model indicates that the nonresonant response of the substrate is out of phase with respect to that of the resonant CO response.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00324005
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  • 7
    Electronic Resource
    Electronic Resource
    Mycelial fragment size distribution: an analysis based on fractal geometry (1993)
    Springer
    Applied microbiology and biotechnology 39 (1993), S. 242-249 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The use of white-rot fungi for biodegradative research and developmental application requires seeding with a suitable inoculum. This paper presents a new method for the quantitative analysis of a mycelial inoculum composed of homogenized hyphal fragments. Our method is premised on a power-law behaviour between frequency and the size of these mycelial fragments. It is shown that the fragment distribution profile can be determined by regression to give the fractal fragmentation dimension, D. The influence of homogenizer speed was also investigated over a range from 8333 rpm to 25 000 rpm, which corresponds to a shear rate range of 13.9 × 103 to 41.7 × 103 s−1. The highest D value was shown at a shear rate of 27.8 × 103 s−1 for 30 s, implying greatest homogeneity in the size distribution function over the measured range (0–500 μm2). As shear force and duration increases (up to a threshold value) the production of small fragments is facilitated with a corresponding decrease in the D value. The slope relation express the fragment diversity whereas the reciprocal fractal valie characterizes the distribution size probability. Image-analysis methodology is described and the implications of a fractal description of a mycelial inoculum are also considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00228613
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  • 8
    Electronic Resource
    Electronic Resource
    Replacement of a conserved glycine residue in subunit II of cytochrome c oxidase interferes with protein function (1994)
    Springer
    Current genetics 25 (1994), S. 233-238 
    Details
    ISSN: 1432-0983
    Keywords: Cytochrome c oxidase ; Respiration deficiency ; Redox center
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In this paper we describe the isolation and characterization of a respiration-deficient yeast strain which is defective in the function of subunit II of cytochrome c oxidase. This strain, VC32, carries a mutation in the mitochondrial COX2 gene which converts a conserved glycine residue to arginine. The conserved glycine is in a region implicated as important for ligating the CuA redox center and for interaction with cytochrome c. We have also characterized five revertants of VC32 which have recovered respiratory function; all five were mapped to the mitochondrial genome. In three of the five revertants the wild-type glycine codon is restored, while in two of the five the mutant arginine codon is still present. These two strains are likely to possess alterations either in components of the mitochondrial translation machinery or in mitochondrially-encoded gene products that interact directly with subunit II to assemble an active oxidase complex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00357167
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  • 9
    Electronic Resource
    Electronic Resource
    Comments on “Nd−Sr isotopic compositions of lower crustal xenoliths-Evidence for the origin of mid-Tertiary felsic volcanics in Mexico” by J. Ruiz, P.J. Patchett, and R.J. Arculus (1990)
    Springer
    Contributions to mineralogy and petrology 104 (1990), S. 609-614 
    Details
    ISSN: 1432-0967
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306668
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  • 10
    Electronic Resource
    Electronic Resource
    The effects of ingestion time of gliclazide in relationship to meals on plasma glucose, insulin and c-peptide levels (1990)
    Springer
    European journal of clinical pharmacology 38 (1990), S. 465-467 
    Details
    ISSN: 1432-1041
    Keywords: Gliclazide ; non-insulin dependent diabetes ; administration time ; plasma glucose ; insulin ; c-peptide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The effect of altering the timing of gliclazide administration in relation to a meal was studied in ten type 2 (non-insulin dependent) chronically treated diabetics. Gliclazide was given 30 min before, at the start of and 30 min after breakfast or omitted altogether. Plasma gliclazide was present at greater than 2 mg/l throughout the study periods. Administration at 30 min after the meal significantly delayed the time to peak for plasma gliclazide. No significant difference was noted in plasma glucose, insulin or c-peptide patterns with any protocol. It is concluded that, in clinical practice, with chronically treated diabetics the timing of gliclazide ingestion in relation to meals is not critical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02336685
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