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  • 1
    Electronic Resource
    Electronic Resource
    Differential toxinogenesis in the genusPichia detected by an anti-yeast killer toxin monoclonal antibody (1991)
    Springer
    Antonie van Leeuwenhoek 59 (1991), S. 139-145 
    Details
    ISSN: 1572-9699
    Keywords: killer toxins ; monoclonal antibodies ; Pichia spp. ; serological analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The differential toxinogenesis of 25 isolates belonging to species of the potential yeast killer genusPichia that were previously classified in the genusHansenula was comparatively demonstrated by two serologic techniques (indirect immunofluorescence and double immunodiffusion) by using a monoclonal antibody against a yeast killer toxin produced by a selected strain ofPichia anomala (UCSC 25F). The killer phenotypes of thePichia isolates were evaluated by their ability to kill each other. The results, although of insufficient taxonomic value for a reliable separation of either species or genera, attest to the genomic heterogeneity for the killer character in the genusPichia as well as the presumptive dual killer/sensitive identity for each single isolate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00580653
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  • 2
    Electronic Resource
    Electronic Resource
    Genomic studies on killer yeasts belonging to the genusPichia (1992)
    Springer
    Antonie van Leeuwenhoek 62 (1992), S. 215-223 
    Details
    ISSN: 1572-9699
    Keywords: DNA fingerprinting ; DNA-DNA hybridization ; killer yeasts ; Pichia spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-four species belonging to the genusPichia were investigated using restriction fragment length polymorphism (RFLP) and Southern blot hybridization of their genomic DNA.Saccharomyces cerevisiae, Kluyveromyces lactis, Williopsis mrakii andCandida albicans were also included in this study. The RFLP patterns were obtained from digestion of yeast DNA with several restriction endonuclease enzymes, and showed various bands with different mobility; in most isolates, the more deeply stained bands were species-specific. This observation was confirmed by the results obtained from Southern blot hybridization of theEcoRI andXhoI RFLP patterns withP. anomala UCSC 25F DNA, digested with the same enzymes, used as probes. These bands are likely to be ribosomal DNA as shown by hybridization of digested DNA from unrelated yeast species (S. cerevisiae, K. lactis andC. albicans). However, one hybridized band, located at 3.9–4.1 Kb, seems to be peculiar to thePichia species. Our study confirms the usefulness of molecular tools in studying genetic relatedness among yeasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582582
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