ISSN:
1432-2048
Schlagwort(e):
Acetabularia
;
Acyl lipid formation
;
Chloroplast (fatty-acid synthesis)
;
Fatty acid (synthesis)
;
Prenyl lipid formation
;
Mevalonate pathway
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Abstract A gentle procedure allowed the isolation of intact and highly active chloroplasts from the unicellular green algaAcetabularia mediterranea. These chloroplasts incorporated carbon from NaH14CO3 into fatty acids and prenyl lipids at a rate of about 20–50 nmol carbon· (mg chlorophyll)−1·h−1. Most of the fatty acids formed in vitro were esterified in galactolipids. The main prenyl lipids synthesized were the chlorophyll side chain, intermediates of the carotenogenic path, α-and β-carotene, as well as lutein. Large amounts of [1-14C]acetate were incorporated, but exclusively into fatty acids.Isopentenyl diphosphate was a good substrate for prenyl-lipid formation in hypotonically treated chloroplasts. The envelope of intact chloroplasts, however, was impermeable to this compound. Intermediates of the mevalonate pathway were not accepted as precursors under conditions whereisopentenyl diphosphate was well incorporated. The results show that the lipid biosynthetic pathways in the plastids ofAcetabularia, a member of the ancient family of Dasycladaceae, are very similar to those in higher-plant plastids.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02411543
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