ISSN:
0006-3592
Schlagwort(e):
hybridoma metabolism
;
perfusion culture
;
suspension culture
;
cell retention
;
antibody productivity
;
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
Notizen:
The steady-state metabolic parameters for a hybridoma cell line have been determined in continuous suspension-perfusion culture over a wide range of perfusion rates and cell bleed rates. Significant increases in viable cell concentrations and volumetric productivities were achieved at high perfusion rates and low cell bleed rates. At the low growth rates examined in this study, cellular metabolism shifted to become more oxidative, and as a result, the fraction of consumed substrate converted to inhibitory metabolic by-products was reduced. Specific antibody productivity was found to be non-growth associated. © 1993 John Wiley & Sons, Inc.
Zusätzliches Material:
13 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bit.260420206
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