Publication Date:
1993-07-30
Description:
A method was developed to clone, without the use of specific functional assays, complementary DNAs (cDNAs) that carry specific amino-terminal signal sequences, such as those encoding intercellular signal-transducing molecules and receptors. The vector used in this system directed the cell surface expression of interleukin-2 receptor fusion proteins when inserts with signal sequences were cloned in-frame with the correct orientation. An expression cDNA library was constructed from a bone marrow stromal cell line, which contained 5' portion-enriched cDNAs (the average size was 400 base pairs). Two cDNAs that encoded putative cytokine molecules, stromal cell-derived factor-1 alpha (SDF-1 alpha) and SDF-1 beta, which belong to the intercrine-macrophage inflammatory protein superfamily, were cloned.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tashiro, K -- Tada, H -- Heilker, R -- Shirozu, M -- Nakano, T -- Honjo, T -- New York, N.Y. -- Science. 1993 Jul 30;261(5121):600-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Medical Chemistry, Kyoto University Faculty of Medicine, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8342023" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Base Sequence
;
Cell Line
;
Chemokine CXCL12
;
*Chemokines, CXC
;
Cloning, Molecular/*methods
;
Cytokines/chemistry/*genetics
;
Genetic Vectors
;
Molecular Sequence Data
;
Open Reading Frames
;
Protein Sorting Signals/chemistry/*genetics/metabolism
;
Receptors, Interleukin-2/genetics
;
Recombinant Fusion Proteins/biosynthesis
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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