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  • Analytical Chemistry and Spectroscopy  (12)
  • difference between cellular and plasma forms  (1)
  • Wiley-Blackwell  (13)
  • Cell Press
  • 1990-1994  (5)
  • 1980-1984  (8)
Collection
Keywords
Publisher
  • Wiley-Blackwell  (13)
  • Cell Press
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Derivatization of β-propiolactone in biological mixtures for enhanced gas chromatographic mass spectrometric determinations (1981)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 8 (1981), S. 327-331 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Detection and identification of low levels (≤200 ppb) of free β-propiolactone in complex biological mixtures were achieved through in situ chemical derivatization and subsequent gas chromatographic mass spectrometric determination of the products. β-Propiolactone, when reacted with octadecylamine, followed by trimethylsilylation, yielded a derivative which exhibited both good gas chromatographic characteristics and highly interpretable mass spectrometric data.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200080802
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  • 2
    Electronic Resource
    Electronic Resource
    Characterization of hydroxyeicosatetraenoic acids and hydroxyeicosatetraenoic acid phosphatidylcholines by liquid secondary ion tandem mass spectrometry (1994)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 23 (1994), S. 399-405 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Arachidonic acid is oxidized to regioisomeric 5(S)-, 12(S)- and 15(S)-hydroxyeicosatetraenoic acids by the corresponding 5-, 12- and 15-lipoxygenases. These hydroxylated fatty acids can then be incorporated into cellular phos-pholipids. Negative liquid secondary ion tandem mass spectrometry using a high-energy collision regime in a tandem four-sector mass spectrometer was used to characterize regioisomeric hydroxyeicosatetraenoic acids and the corresponding hydroxyeicosatetraenoic phosphatidylcholine species. Collision-induced dissociation (CID) of the [M - H]- negative ion at m/z 319 from the hydroxyeicosatetraenoic acids regioisomers produced some similar product ions, such as m/z 301 [M - H - H2O]- and m/z 257 [M - H - (H2O + CO2)]-. In addition, product ions characteristic of the particular hydroxyeicosatetraenoic acid were formed from α cleavages adjacent to the hydroxyl moieties. Negative liquid secondary ion mass spectrometry of purified hydroxyeicosatetraenoate phosphatidylcholine species gave an ion at m/z 810 [M - CH3] -. CID of the m/z 810 ion gave product ions at m/z 283 and m/z 319, corresponding to stearate at the sn-1 position and hydroxyeicosatetraenoate at the sn-2 position, respectively. From CID of the negative ion at m/z 319 and examination of the product ion spectra, the hydroxyeicosatetraenoate regioisomer present in the phosphatidylcholine could be identified.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200230704
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of the malondialdehyde-2′-deoxyguanosine adduct in rat liver DNA by gas chromatography/electron capture negative chemical ionization mass spectrometry (1994)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 23 (1994), S. 457-464 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct formed. We have developed a sensitive analytical method to characterize and quantify M1G-dR from biological matrices using gas chromatogrphy/electron capture negative chemical ionization mass spectrometry (GC/ECNCI MS). Reduction of M1G-dR with sodium borohydride produced a dihydro derivative (H2-M1G-dR). This more stable analog had improved high-performance liquid chromatographic characteristics which facilitated its isolation from biological fluids. H2-M1G-dR was converted to a mono-pentafluorobenzyl derivative with simultaneous depurination; it was then converted to the corresponding t-butyldimethylsilyl derivative and analyzed by GC/ECNCI MS. (2H2)H2-M1G was used as internal standard. Quantitative analysis was carried out using selected ion monitoring of m/z 302 and m/z 304 where the limit of detection was 10 pg (30 fmol) injected on-column. The level of M1G-dR in normal rat liver was 5.2 ± 0.2 modified bases per 107 bases (n = 6 rats).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200230802
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  • 4
    Electronic Resource
    Electronic Resource
    Characterization of in vitro metabolites of the antipsychotic drug tiospirone by mass spectrometry (1990)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 19 (1990), S. 281-285 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Metabolism of the antipsychotic drug tiospirone was studied in vitro with phenobarbital-induced rat liver microsomes. Metabolites were isolated and purified to homogeneity by high-performance liquid chromatography. It was possible to characterize the metabolites as trimethylsilyl (TMS) derivatives by gas chromatography/electron impact mass specrometry (GC/EIMS) so long as the sulfur was present in the reduced form. However, sulfoxide and sulfone analogs of tiospirone underwent reductive decomposition on the GC column. In addition, no molecular ions were observed in the EI spectra of these analogs. Desorption chemical ionization/mass spectrometry (DCI/MS) in the positive ion mode with methane as reagent gas successfully distinguished sulfoxides and sulfones from their parent sulfides. Protonated molecular ions were observed together with structurally significant fragment ions. Five microsomal metabolites of tiospirone were characterized by a combination of GC/EIMS and DCI/MS. From the structures of the metabolites three major pathways of metabolism were identified: N-dealkylation of the butyl side chain at the piperazinyl nitrogen, hydroxylation α to the glutarimidyl carbonyl at C-6 on the azaspirodecanedione ring, and sulfoxide formation on the benzisothiazole moiety.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200190502
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of effector cell-derived lyso platelet activating factor by electron capture negative ion mass spectrometry (1991)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 20 (1991), S. 545-552 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Quantification of 1-O-alkyl-2-lyso-sn-3-glycero-phosphocholine (lysoPAF) and determination of the different molecular species released by cells has been hampered by the molecules's lack of intrinsic bioactivity, unavailability of a suitable internal standard, and reliance on derivatives requiring electron impact techniques. We have synthesized trideuterated internal standards (labeled on the terminal carbon of the alkyl chain) for both C16:0 and C18:0 lysoPAF. Using these standards, we isolated and quantified lysoPAF released from A23187-stimulated human neutrophils and rat alveolar macrophages. Extracted lysoPAF was purified by solid-phase extraction and thin-layer chromatography. The polar phosphorylcholine group was removed with 29 M HF or phospholipase C. The two free hydroxyl groups were derivatized with pentafluorobenzoyl chloride. The resultant bis-pentafluorobenzoyl derivative, analyzed by gas chromatography/electron capture negative ion mass spectrometry, underwent substantial fragmentation. Lowering of the ion source temperature resulted in a dramatic increase in signal-to-noise ratio, with the vast majority of the ion current carried in the molecular anion. Stimulated neutrophils released 16.3 and 10.2 ng/106 cells of C16:0 lysoPAF and C18:0 lysoPAF, respectively. Rat macrophages synthesized 15.9 ng/106 cells of C16:0 lysoPAF, but C18:0 lysoPAF was variably detected at low levels. We conclude that use of the bispentafluorobenzoyl ester derivative of lysoPAF allows facile quantification of this autacoid metabolite in biological matrices.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200200907
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  • 6
    Electronic Resource
    Electronic Resource
    Enantiospecific quantification of hexobarbital and its metabolites in biological fluids by gas chromatography/electron capture negative ion chemical ionization mass spectrometry (1991)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 20 (1991), S. 559-564 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A highly sensitive and specific assay based on gas chromatography/electron capture negative ion chemical ionization mass spectrometry has been developed for the analysis of the enantiomers of hexobarbital and its major metabolites in human urine and plasma. S-(+)-(5-2H3)hexobarbital and R-(-)-(5-2H3)hexobarbital were synthesized for clinical studies along with (±)-(1,5-2H6)hexobarbital and the deuterated major metabolites for use as internal and reference standards. Hexobarbital enantiomers and their metabolites were analyzed after pentafluorobenzyl and trimethylsilyl derivatization, following solid-phase extraction from plasma and urine. Intense negative ion spectra were observed for all of the derivatives. The base peak in the spectra corresponded to the M - penta-fluorobenzyl anion [M — PFB]- except for 1,5-dimethylbarbituric acid, where M-· was the most abundant ion. The applicability of the method was demonstrated by following the plasma concentration-time profiles and urinary excretion in a male extensive metabolizer of mephenytoin who was given a pseudoracemic oral dose of hexobarbital containing equal 50 mg amounts of S-(+)-2(H0)hexobarbital and R-(-)-(2H3)hexobarbital. Marked stereoselective disposition was observed, with the R-(-)-enantiomer being more efficiently metabolized, primarily by alicyclic oxidation and ring cleavage.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200200909
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  • 7
    Electronic Resource
    Electronic Resource
    Peptide studies using a fast atom bombardment high field mass spectrometer and data system. 2 - characteristics of positive ionization spectra of peptides, m/z 858 to m/z 5729 (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 408-419 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Sixteen peptides ranging in molecular weights from 858 to 5729 were examined under positive ionization fast atom bombardment conditions employing a high field magnet mass spectrometer and data system. The contributions of the polyisotopic elements to the molecular protonated ion envelope become important in the interpretation of the data at higher mass. For masses less than 2000 u, the most abundant ion within this envelope is the monoisotopic molecular protonated ion. Above 2000 u, the most abundant ion in the envelope is a polyisotopic molecular protonated ion. Characterization of the peptide requires identification of both the molecular protonated ion envelope and significant fragment ions. Partial spectra displaying both the molecular ion and significant fragment ions are presented for mastoparan (mol. wt = 1478), somatostatin (mol. wt = 1637), bovine parathyroid hormone (1-34) (mol. wt = 4106), and bovine insulin (mol. wt = 5729). A partial spectrum for bovine ribonuclease A (mol. wt = 13673) displayed significant fragment ions that identified the protein. The types of fragment ions included those that indicated the amino acid sequence and the location of disulfide bonds. The abundance of these ions appears to be influenced by the characteristics of the noble gas fast atom beam and the sample matrix.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100705
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  • 8
    Electronic Resource
    Electronic Resource
    Peptide studies using a fast atom bombardment high field mass spectrometer and data system: 3 - negative ionization: Mass calibration, data acquisition and structural characterization (1983)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 10 (1983), S. 387-393 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Under negative ionization conditions, nominal mass calibration of the fast atom bombardment high field mass spectrometer and data system was accomplished using cesium iodide/glycerol as a reference. Mass calibration at -8 kV accelerating potential extends from m/z 387 to m/z 2170 using xenon fast atoms. Negative xenon FAB mass spectra for human angiotensin I and human gastrin I complement their positive fast atom bombardment spectra. Negative xenon fast atom bombardment spectra of underivatized peptides exhibit molecular proton-abstracted ion envelopes and structurally significant fragment ions. Peptide mixture analysis under negative xenon fast atom bombardment reveals peptide molecular ion envelopes of higher relative intensities than under positive xenon fast atom bombardment.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200100609
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  • 9
    Electronic Resource
    Electronic Resource
    Ion cyclotron resonance studies of alkylsilyl ions: V - The reactions of alcohols and ethers with the allyldimethylsilyl cationPart IV: J. Chem. Soc. Perkin Trans. 2 in press. Also part CXXVII in the series Electron Impact Studies. Part CXXVI: J. Chem. Soc. Chem. Commun. 230 (1979). (1980)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 15 (1980), S. 15-17 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Diallyldimethylsilane provides a source of the allyldimethylsilyl cation in the ion cyclotron resonance spectrometer; reaction of this cation with alcohols (ROH) produces adducts which decompose by loss of C3H6 to yield the ion \documentclass{article}\pagestyle{empty}\begin{document}$ \left[{{\rm Me}_2 \mathop {\rm S}\limits^{\rm + } {\rm i} - {\rm OR}} \right] $\end{document}. This elimination is thought to occur by a 1,5-hydrogen shift, together with either stepwise or concerted silicon-carbon bond cleavage. The corresponding aducts from ethers R—O—R1 (R1≥R, R1≥Et) first lose (R1—H·) and then undergo the elimination described above.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210150107
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  • 10
    Electronic Resource
    Electronic Resource
    Structural analysis of fibronectin with monoclonal antibodies (1981)
    New York, NY : Wiley-Blackwell
    Journal of Supramolecular Structure and Cellular Biochemistry 17 (1981), S. 153-161 
    Details
    ISSN: 0275-3723
    Keywords: difference between cellular and plasma forms ; fibronectin ; monoclonal antibodies ; structure and function ; Chemistry ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: The reactivity of six monoclonal antibodies with fragments of fibronectin produced with trypsin, chymotrypsin, and Staphylococcus aureus V8 protease is described. All these antibodies reacted with fragments derived from the C-terminal one-third of fibronectin. This region probably contains sites for the binding of fibronectin to cells, and to heparin and may also contain active sites for the reattachment, spreading, and alignment of transformed cells. Analysis of the reactivities of different sets of proteolytic fragments with the antibodies and with other ligands (eg. heparin) allows one to determine overlaps between the fragments and to locate the positions of the different binding sites for antibodies and ligands. One of the antibodies has allowed us to identify a site of structural difference between cellular and plasma fibronectins from hamsters. The site recognized by this antibody is located near to, but not at, the C-terminal end and docs not involve carbohydrate groups. Because of its internal location in fibronectin, this difference suggests that there are probably different genes for cellular and plasma fibronectin. These monoclonal antibodies should be useful for further probing the functions present in the C-terminal regions of fibronectin and for determining their locations.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jsscb.380170206
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