Publication Date:
1992-07-03
Description:
Medium-chain fatty acids (FAs), found in storage lipids of certain plants, are an important renewable resource. Seeds of undomesticated California bay accumulate laurate (12:0), and a 12:0-acyl-carrier protein thioesterase (BTE) has been purified from this tissue. Sequencing of BTE enabled the cloning of a complementary DNA coding for a plastid-targeted preprotein. Expression of the complementary DNA in the seeds of Arabidopsis thaliana resulted in BTE activity, and medium chains accumulated at the expense of long-chain (greater than or equal to 16) FAs. Laurate became the most abundant FA species and was deposited in the storage triacylglycerols. These results demonstrate a mechanism for medium-chain FA synthesis in plants.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Voelker, T A -- Worrell, A C -- Anderson, L -- Bleibaum, J -- Fan, C -- Hawkins, D J -- Radke, S E -- Davies, H M -- New York, N.Y. -- Science. 1992 Jul 3;257(5066):72-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Calgene, Inc., Davis, CA 95616.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/1621095" target="_blank"〉PubMed〈/a〉
Keywords:
Acetyltransferases/genetics/*metabolism
;
Acyl-Carrier Protein S-Acetyltransferase
;
Amino Acid Sequence
;
DNA/genetics
;
Fatty Acids/*biosynthesis/isolation & purification
;
Genetic Engineering
;
Lauric Acids/*metabolism
;
Molecular Sequence Data
;
Plants/genetics/*metabolism
;
Plants, Genetically Modified
;
Plasmids
;
Seeds/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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