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Genetic and biochemical studies on glutamate-pyruvate transaminase from Drosophila melanogaster (1980)

Leigh Brown, Andrew J. ; Voelker, Robert A.

Springer

Biochemical genetics 18 (1980), S. 303-309

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ISSN: 1573-4927

Keywords: Drosophila melanogaster ; allozymes ; GPT ; genetic mapping

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Chemistry and Pharmacology

Notes: Abstract We have used electrophoretic variants of glutamate-pyruvate transaminase (GPT, E.C. 2.6.1.2) in Drosophila melanogaster to genetically map the structural gene to position 42.6 on the X chromosome. By pseudodominance tests over several deficiencies we have localized it cytogenetically to the interval 11Fl-2 to 12Al-2. The sedimentation constant (s 20,w) of the native enzyme was determined in sucrose density gradients to be 5.9 and the native molecular weight approximately 87,000. The similarity in physical properties to mammalian enzymes suggests that the enzyme may also be dimeric in D. melanogaster.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00484243

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Lectin binding and uptake and glycoprotein characterization of isolated porcine aortic endothelial and smooth muscle cells (1993)

Schumacher, Udo ; Vischer, Peter ; Völker, Wolfgang ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Cell Biochemistry and Function 11 (1993), S. 225-230

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ISSN: 0263-6484

Keywords: Electrophoresis ; endothelia ; glycoproteins ; lectins ; smooth muscle cells ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Medicine

Notes: Endothelial and smooth muscle cells were isolated from porcine aorta and kept in short-term culture. To determine the terminal carbohydate composition of the plasma membranes from both cell populations, the cells were incubated with a panel of fluorescein-labelled lectins. Both cell populations shared a number of terminal carbohydrates, but the N-galactosamine specific lectin Wistaria floribunda agglutinin labelled only the endothelial cells. A lectin which selectively labelled smooth muscle cells was not found. Western blot analysis of isolated endothelial cell membrane glycoproteins indicated that most membrane glycoproteins are labelled by Wistaria floribunda agglutinin.

Additional Material: 4 Ill.