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  • Polymer and Materials Science  (11)
  • Saccharomyces cerevisiae  (3)
  • 1990-1994  (5)
  • 1985-1989  (9)
  • 1
    Electronic Resource
    Electronic Resource
    Effects of m-Cl-peroxy benzoic acid on glycolysis in Saccharomyces cerevisiae (1985)
    Springer
    Archives of microbiology 143 (1985), S. 220-224 
    Details
    ISSN: 1432-072X
    Keywords: Peroxy benzoic acid ; Saccharomyces cerevisiae ; ATP ; Glycolysis ; Glyceraldehyde-3-phosphate dehydrogenase ; Colony forming capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Concentrations of m-Cl-peroxy benzoic acid (CPBA) higher than 0.1 mM decrease the ATP-content of Saccharomyces cerevisiae in the presence of glucose in 1 min to less than 10% of the initial value. In the absence of glucose, 1.0 mM CPBA is necessary for a similar effect. After the rapid loss of ATP in the first min in the presence of glucose caused by 0.2 mM CPBA, the ATP-content recovers to nearly the initial value after 10 min. Aerobic glucose consumption and ethanol formation from glucose are both completely inhibited by 1.0 mM CPBA. Assays of the activities of nine different enzymes of the glycolytic pathway as well as analysis of steady state concentrations of metabolites suggest that glyceraldehyde-3-phosphate dehydrogenase is the most sensitive enzyme of glucose fermentation. Phosphofructokinase and alcohol dehydrogenase are slightly less sensitive. Incubation for 1 or 10 min with concentrations of 0.05 to 0.5 mM CPBA causes a) inhibition of glyceraldehyde-3-phosphate dehydrogenase, b) decrease of the ATP-content and c) a decrease of the colony forming capacity. From these findings it is concluded that the disturbance of the ATP-producing glycolytic metabolism by inactivation of glyceraldehyde-3-phosphate dehydrogenase may be an explanation for cell death caused by CPBA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411239
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of ozone on ATP, cytosolic enzymes and permeability of Saccharomyces cerevisiae (1987)
    Springer
    Archives of microbiology 147 (1987), S. 105-108 
    Details
    ISSN: 1432-072X
    Keywords: Ozone ; Yeast ; Saccharomyces cerevisiae ; ATP ; Nucleotides ; Permeability ; Cytosolic enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of a yeast suspension with ozone inactivates a number of cytosolic enzymes. Among 15 studied, the most drastic inactivation was found for glyceraldehyde-3-phosphate dehydrogenase and to lesser extents: NAD-glutamate dehydrogenase, pyruvate decarboxylase, phosphofructokinase-1 and NAD-alcohol dehydrogenase. Ozone treatment also effects the quantity of ATP and of other nucleoside triphosphates, reducing to about 50% of the initial value. The ATP missing in the cells appears in the medium. NAD and protein also accumulate in the medium suggesting that the yeast cells have been permeabilized. Permeabilization of the yeast cells by treatment with ozone preceeds the inactivation of glyceraldehyde-3-phosphate dehydrogenase and other cytosolic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415269
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  • 3
    Electronic Resource
    Electronic Resource
    Topographical requirements for δ-selective opioid peptidesAbbreviations: All amino acids are of the L variety unless otherwise stated. Abbreviations for amino acid residues follow those recommended by the IUPAC. Other abbreviations include the following: DPDPE. enkephalin, H-Tyr-D- Pen, penicillamine or β,β-dimethylcysteine; DCFPE, DRE, dermen kephalin, H-Tyr-D-Met-Phe-His-Leu-Met-Asp-NHg; DPLPE, DSLET, H-Tyr-D-Ser-Gly-Phe-Leu-Thr-OH; DSTBULET, H-Tyr-D-Ser(OtBu)-Gly-Phe-Leu-Thr-OH; BUBU, H-Tyr-D-Ser(OtBu)-Gly-Phe-Leu-Thr(OtBu)-OH; DAGO, [D -ala2, N-MePhe4, Gly5]enkephalin-OL. (1991)
    New York : Wiley-Blackwell
    Biopolymers 31 (1991), S. 941-955 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformational possibilities of three different δ-selective opioid peptides, which are DPDPE , and DRE (Tyr-D-Met-Phe-His-Leu-Met-Asp-NH2, dermenkephalin), were explored using energy calculations. Sets of low-energy conformers were obtained for each of these peptides. The sets consisted of 61 structures for DPDPE, 32 for DCFPE, and 38 for DRE, including various types of rotamers of the Tyr and Phe side-chain groups. Comparison of the geometrical shapes of the conformers was performed for these sets using topographical considerations, i.e., examination of the mutual spatial arrangement of the N-terminal α-amino group, and of the Tyr and Phe side-chain groups. The results obtained suggest a model for the δ-receptor-bound conformer(s) for opioid peptides. The model suggests the placement of the Phe side chain in a definite position in space corresponding to the g- rotamer of Phe for peptides containing Phe4 and to the t rotamer for peptides containing Phe.3 The position of the Tyr1 side chain cannot be specified so precisely. The proposed model is in a good agreement with the results of biological testing of β-Me-Phe4-substituted DPDPE analogues that were not considered in the process of model construction.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.360310804
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  • 4
    Electronic Resource
    Electronic Resource
    Synthese verschiedener Oxincellulosen für spurenanalytische Anreicherungsverfahren (1989)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 170 (1989), S. 159-172 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Some routes to synthetize covalently bound chelating groups as 8-hydroxyquinoline or 8-hydroxyquinoline-5-sulfonic acid to cellulose are given. The products show a capacity of 500-800 μmol/g, fast kinetics of exchange and good stability against the eluent. The apparent stability constant of the complexes with Zn2+ are around 2 · 105 mol-1 dm3.
    Notes: Es werden mehrere Synthesemöglichkeiten für die Herstellung von an Cellulose immobilisierten 8-Hydroxychinolin- und 8-Hydroxychinolin-5-sulfonsäure-Derivaten beschrieben, die zu einem Produkt mit einer Kapazität von 500-800 μmol/g, hoher Austauschgeschwindigkeit und guter Stabilität gegenüber dem Eluenten führen. Die scheinbare Komplexbildungskonstante der Oxincellulosen mit Zn2+ ist etwa 2 · 105 mol-1 dm3.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1989.051700112
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  • 5
    Electronic Resource
    Electronic Resource
    Hydrodynamic behaviour of 1,4-trans-polyisoprene (1985)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 186 (1985), S. 2341-2353 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The unperturbed dimensions of 1,4-trans-polyisoprene (TPIP) were estimated using various [η] - Mw extrapolation methods. The results of various methods agree very closely, however, the solvent-polymer interaction parameter, B, assumes different values from method to method. The polymer chains are found to assume random coil structure in solvents like benzene, toluene and cyclohexane. A more compact coiled structure is found in hexane. This was further substantiated by estimation of the excluded volume per segment: 7,3 Å3 in good solvents and 4,0 Å3 in hexane. On a relative scale, the stiffness parameters for TPIP chains were found to be: σ = 1,26 and C∞ = 5,99 for the expanded coil configuration, and σ = 1,11 and C∞ = 4,46 for the compact coil configuration, which is smaller than the literature data. If the latter are corrected for molecular heterogeneity, the agreement with the present data is excellent. The temperature coefficient of the unperturbed dimensions also supports this results.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1985.021861115
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  • 6
    Electronic Resource
    Electronic Resource
    Crystallization kinetics of 1,4-trans-polyisoprene, 1. Pure polymer (1987)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 188 (1987), S. 421-431 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The Crystallization kinetics of an unfractionated sample of 1,4-trans-polyisoprene was studied from its melt by means of the dilatometric method. The data are analysed according to the secondary nucleation theory. A critical discussion and comparison of previously published kinetic parameters with those derived in the present study is made. The values of the product of interfacial free energies, σσe, were found to be 590,5 and 915 erg2/cm4 for the low melting and high melting polymorphs, respectively. The observed deviation from the Avrami theory towards completion of the process is interpreted to be due to the low molecular weight species of the low melting polymorph.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1987.021880218
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  • 7
    Electronic Resource
    Electronic Resource
    Crystallization kinetics of 1,4-trans-polyisoprene, 2Part 1: cf.8.. Polymer-diluent mixtures (1987)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 188 (1987), S. 433-444 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Four mixtures of 1,4-trans-polyisoprene with methyl oleate (vol. fraction of polymer: 0,696 to 0,092) were prepared, and a dilatometric study of the crystallization kinetics was carried out in a convenient temperature range. The polymer was found to crystallize in the form of its high melting polymorph. The amount of secondary crystallization was less compared to that observed earlier for undiluted polymer. Formation of crystalline phase was found to be more difficult. In the absence of nucleation and growth data, the actual differences in interfacial energies required for nucleation and growth could not be estimated quantitatively, however, the experimental results indicate that compared to the bulk system, the energy requirements for the primary nucleation act in case of mixtures are higher. The interfacial energy σ′e (calculated after neglecting the transport term) was in the range 2309-2356 cal/mol for all the mixtures and showed appreciable variation with change in polymer concentration. A molecular interpretation of the possible mechanism operating in the crystallization of mixtures is given.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1987.021880219
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  • 8
    Electronic Resource
    Electronic Resource
    Synthesis and properties of polyurethanes containing s-triazine rings in the main chainNCL Communication No. 4557. (1989)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 27 (1989), S. 3263-3269 
    Details
    ISSN: 0887-624X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Eight new diisocyanate monomers containing s-triazine ring have been prepared from the parent diacids via the Curtius rearrangement of the corresponding diacyl azides. The parent diacids were synthesised by the reaction of p- and m-hydroxybenzoic acid with 6-methoxy-2,4-dichloro-s-triazine, 6-phenoxy-2,4-dichloro-s-triazine, 6-phenyl-2,4-dichloro-s-triazine, and 2-diphenylamino-4,6-dichloro-s-triazine respectively. Polyurethanes have been synthesised by solution polymerization of these diisocyanates with ethylene glycol in N,N-dimethylacetamide (DMAC). The resulting polymers were characterized by IR spectroscopy, viscosity measurement, solubility tests, and softening points.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1989.080271007
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  • 9
    Electronic Resource
    Electronic Resource
    A nondenaturing solid phase pharmaceutical carrier comprised of surface-modified nanocrystalline materials (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 1 (1990), S. 289-294 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: Numerous carrier systems have been developed for the controlled delivery of biologically active molecules such as drugs and diagnostic agents. The biophysical interactions between the biologically active molecules and their carriers, however, may denature the former and lead to reduced biological activity. In this study, a model nondenaturing carrier comprised of a nanocrystalline (10-7 m) tin oxide core and a surface-charge-reducing organic bonding layer (GF292) was synthesized. A subsequently bound protein (human transferrin) showed significant retained conformation by immunoelectron microscopy. In the synthesis of targeted drug systems and vaccines, nanocrystalline cores treated with appropriate surface-modifying agents may be suitable carriers.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770010404
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  • 10
    Electronic Resource
    Electronic Resource
    Thermal stability and degradation studies of polyester resins (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 37 (1989), S. 1901-1908 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Thermal behavior and degradation mechanisms of three polyester resins, namely bisphenol A based, isophthalic acid based, and general purpose were studied. These resins were cured with styrene in the presence of benzoyl peroxide (BPO) and methyl ethyl ketone peroxide (MEKP) as initiators. The cured resins were studied by thermogravimetric analysis and mass spectrometry. From the thermogravimetric data it was observed that the cured bisphenol-A-based polyester resin was thermally more stable than the cured isophthalic acid-based and general purpose polyester resins. It was also observed that polyester resins cured with MEKP are thermally more stable than benzoyl peroxide-cured products. Thermogravimetric analysis data shows that there are two first-order degradation reactions during thermal degradation in nitrogen atmosphere. The first degradation step involves the scission of cross-links/weak links with the liberation of free linear chains. The second step involves a random scission of the free linear chains into smaller fragments. The framentation patterns of the polyester resins were identified by mass spectrometery. Possible mechanisms for the degradation of the cured polyesters accounting for the experimental observations are discussed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1989.070370713
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