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  • 1
    ISSN: 1573-6822
    Keywords: blebbing ; cell death ; cytoskeleton ; mitochondria ; oxidative stress ; t-BHP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effects of the oxidizing agent t-butylhydroperoxide (t-BHP) were investigated on three human cell lines of different origin and growth features (A431 epithelial cells, ADF astrocytoma cells and U937 leukemic cells) using electron microscopy and electron paramagnetic resonance spectroscopy. The results indicate that important biophysical and ultrastructural modifications are induced in the plasma and mitochondrial membranes of these cells and that these changes can ultimately lead to cell death. In addition, the cell cytoskeleton also appears to be a target of hydroperoxide-mediated stress. In particular, all three cell types undergo cytoskeletal alterations leading to surface blebbing, a typical characteristic of cell damage. However, the timing and extent of this damage as well as that occurring at the mitochondrial and plasma membrane levels seems to be different: cells with weak (ADF) or absent (U937) cell-to-cell and cell-substrate contacts and a poorly developed cytoskeleton appear to be more susceptible than other cell types (e.g., A431) to t-BHP-mediated injury. These diverse cell susceptibilities to hydroperoxide-mediated oxidative stress could thus depend upon cell histotype-associated growth featurs.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Cell biology and toxicology 9 (1993), S. 119-130 
    ISSN: 1573-6822
    Keywords: cell death ; cell membrane ; free radicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Plasma membrane modifications have been widely recognized as crucial factors in cell injury and death. One of these modifications, surface blebbing, has been considered as an injury-marker associated with a series of biochemical and physiological modifications. Our study focused on the different effects of free radical-induced cell damage by quinone menadione (2-methyl-1,4-naphthoquinone) and by hyperthermic shock (45°C) on the erythroleukemic cell line K.562. Different techniques including immunofluorescence, freeze-fracturing, and electron paramagnetic resonance spectroscopy were employed. Menadione induced the formation of surface blebs, accompanied by a rearrangement of the microfilament system and changes in the distribution of plasma membrane proteins. In contrast, heat-shocked cells showed neither blebbing nor important cytoskeletal changes. Finally, the electron paramagnetic resonance results showed an increase in membrane order not specifically related to the type of free radical-induced stress. These cell death features appear to suggest the existence of two different types ofpathways for necrotic cell death: both treatments induce cell injury and eventual death by modifiting plasma membrane integrity and function. However, one involves cytoskeleton-dependent surface blebbing, whereas the other does not.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 2024-01-12
    Description: Amphipods from the Portuguese south and south-western continental shelf were studied with regard to the species inventory, distribution, and ecology. This study allowed the identification of 113 species, belonging to 52 genera; of these species 28 are recorded for the first time along the Portuguese coast. Two species, Ampelisca heterodactyla Schellenberg, 1925, and A. latifrons Schellenberg, 1925, are redescribed. The latter is different from the Ampelisca named latifrons by Ledoyer in 1972 and 1982.\nThe absolute frequencies and abundances of the species, and their distribution in terms of bathymetry and type of substrate are also analysed. The most frequent and abundant species also presented wide ranges of vertical distribution. In addition, these species are preferentially found in medium or fine sand bottoms. The granulometric structure of the habitats might, therefore, be the most important factor conditioning the development of amphipod populations. The group of the most frequent and abundant species was dominated by Ampelisca. Thus, at least in the shelf sediments, the species of this genus might have a key role as food for many secondary consumers, especially demersal fishes.
    Keywords: Gammaridea ; Caprellidea ; taxonomy ; distribution ; ecology ; Portugal
    Repository Name: National Museum of Natural History, Netherlands
    Type: info:eu-repo/semantics/article
    Format: application/pdf
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