ISSN:
1617-4623
Keywords:
Azospirillum
;
Cloning
;
Heterospecific expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A gene bank from Azospirillum brasilense, Sp6 strain, was constructed in Escherichia coli in plasmid pRK290 and was used to identify Azospirillum genes. Clones carrying the his1, his2, pyr and cys1 genes were identified by genetic complementation and the expression of A. brasilense his1, his2 and pyr genes in E. coli was demonstrated. By E. coli complementation experiments, a cluster of three genes for the histidine biosynthetic pathway in A. brasilense has also been found, suggesting the existence of an operon-like unit.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00326539
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