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  • Analytical Chemistry and Spectroscopy  (10)
  • Saccharomyces cerevisiae  (3)
  • 1990-1994  (4)
  • 1985-1989  (9)
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Keywords
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Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Effects of m-Cl-peroxy benzoic acid on glycolysis in Saccharomyces cerevisiae (1985)
    Springer
    Archives of microbiology 143 (1985), S. 220-224 
    Details
    ISSN: 1432-072X
    Keywords: Peroxy benzoic acid ; Saccharomyces cerevisiae ; ATP ; Glycolysis ; Glyceraldehyde-3-phosphate dehydrogenase ; Colony forming capacity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Concentrations of m-Cl-peroxy benzoic acid (CPBA) higher than 0.1 mM decrease the ATP-content of Saccharomyces cerevisiae in the presence of glucose in 1 min to less than 10% of the initial value. In the absence of glucose, 1.0 mM CPBA is necessary for a similar effect. After the rapid loss of ATP in the first min in the presence of glucose caused by 0.2 mM CPBA, the ATP-content recovers to nearly the initial value after 10 min. Aerobic glucose consumption and ethanol formation from glucose are both completely inhibited by 1.0 mM CPBA. Assays of the activities of nine different enzymes of the glycolytic pathway as well as analysis of steady state concentrations of metabolites suggest that glyceraldehyde-3-phosphate dehydrogenase is the most sensitive enzyme of glucose fermentation. Phosphofructokinase and alcohol dehydrogenase are slightly less sensitive. Incubation for 1 or 10 min with concentrations of 0.05 to 0.5 mM CPBA causes a) inhibition of glyceraldehyde-3-phosphate dehydrogenase, b) decrease of the ATP-content and c) a decrease of the colony forming capacity. From these findings it is concluded that the disturbance of the ATP-producing glycolytic metabolism by inactivation of glyceraldehyde-3-phosphate dehydrogenase may be an explanation for cell death caused by CPBA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00411239
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of ozone on ATP, cytosolic enzymes and permeability of Saccharomyces cerevisiae (1987)
    Springer
    Archives of microbiology 147 (1987), S. 105-108 
    Details
    ISSN: 1432-072X
    Keywords: Ozone ; Yeast ; Saccharomyces cerevisiae ; ATP ; Nucleotides ; Permeability ; Cytosolic enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of a yeast suspension with ozone inactivates a number of cytosolic enzymes. Among 15 studied, the most drastic inactivation was found for glyceraldehyde-3-phosphate dehydrogenase and to lesser extents: NAD-glutamate dehydrogenase, pyruvate decarboxylase, phosphofructokinase-1 and NAD-alcohol dehydrogenase. Ozone treatment also effects the quantity of ATP and of other nucleoside triphosphates, reducing to about 50% of the initial value. The ATP missing in the cells appears in the medium. NAD and protein also accumulate in the medium suggesting that the yeast cells have been permeabilized. Permeabilization of the yeast cells by treatment with ozone preceeds the inactivation of glyceraldehyde-3-phosphate dehydrogenase and other cytosolic enzymes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415269
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of effector cell-derived lyso platelet activating factor by electron capture negative ion mass spectrometry (1991)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 20 (1991), S. 545-552 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Quantification of 1-O-alkyl-2-lyso-sn-3-glycero-phosphocholine (lysoPAF) and determination of the different molecular species released by cells has been hampered by the molecules's lack of intrinsic bioactivity, unavailability of a suitable internal standard, and reliance on derivatives requiring electron impact techniques. We have synthesized trideuterated internal standards (labeled on the terminal carbon of the alkyl chain) for both C16:0 and C18:0 lysoPAF. Using these standards, we isolated and quantified lysoPAF released from A23187-stimulated human neutrophils and rat alveolar macrophages. Extracted lysoPAF was purified by solid-phase extraction and thin-layer chromatography. The polar phosphorylcholine group was removed with 29 M HF or phospholipase C. The two free hydroxyl groups were derivatized with pentafluorobenzoyl chloride. The resultant bis-pentafluorobenzoyl derivative, analyzed by gas chromatography/electron capture negative ion mass spectrometry, underwent substantial fragmentation. Lowering of the ion source temperature resulted in a dramatic increase in signal-to-noise ratio, with the vast majority of the ion current carried in the molecular anion. Stimulated neutrophils released 16.3 and 10.2 ng/106 cells of C16:0 lysoPAF and C18:0 lysoPAF, respectively. Rat macrophages synthesized 15.9 ng/106 cells of C16:0 lysoPAF, but C18:0 lysoPAF was variably detected at low levels. We conclude that use of the bispentafluorobenzoyl ester derivative of lysoPAF allows facile quantification of this autacoid metabolite in biological matrices.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200200907
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  • 4
    Electronic Resource
    Electronic Resource
    Enantiospecific quantification of hexobarbital and its metabolites in biological fluids by gas chromatography/electron capture negative ion chemical ionization mass spectrometry (1991)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 20 (1991), S. 559-564 
    Details
    ISSN: 1052-9306
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A highly sensitive and specific assay based on gas chromatography/electron capture negative ion chemical ionization mass spectrometry has been developed for the analysis of the enantiomers of hexobarbital and its major metabolites in human urine and plasma. S-(+)-(5-2H3)hexobarbital and R-(-)-(5-2H3)hexobarbital were synthesized for clinical studies along with (±)-(1,5-2H6)hexobarbital and the deuterated major metabolites for use as internal and reference standards. Hexobarbital enantiomers and their metabolites were analyzed after pentafluorobenzyl and trimethylsilyl derivatization, following solid-phase extraction from plasma and urine. Intense negative ion spectra were observed for all of the derivatives. The base peak in the spectra corresponded to the M - penta-fluorobenzyl anion [M — PFB]- except for 1,5-dimethylbarbituric acid, where M-· was the most abundant ion. The applicability of the method was demonstrated by following the plasma concentration-time profiles and urinary excretion in a male extensive metabolizer of mephenytoin who was given a pseudoracemic oral dose of hexobarbital containing equal 50 mg amounts of S-(+)-2(H0)hexobarbital and R-(-)-(2H3)hexobarbital. Marked stereoselective disposition was observed, with the R-(-)-enantiomer being more efficiently metabolized, primarily by alicyclic oxidation and ring cleavage.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200200909
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  • 5
    Electronic Resource
    Electronic Resource
    Mass spectra of some 2-(3′,5′-diphenylpyrazol-1′-yl)-6-substituted Benzothiazoles (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 22 (1987), S. 49-51 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210220113
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  • 6
    Electronic Resource
    Electronic Resource
    Mass spectra of some 1-(6′-substituted-4′-methyl-2′-quinolyl)-3-methylpyrazol-5-ols and their 4-substituted analogues (1987)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 22 (1987), S. 212-215 
    Details
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Electron impact induced fragmentation of some 1-(6′-substituted-4′-metbyI-2′-quinolyI)-3-methylpyrazoI-5-ols follows a route where the pyrazole moiety is preferentially cleaved with successive losses of two moieties of 41 u. High-resolution measurements have established that the first loss is due to the Ċ2HO moiety, which necessitates an intramolecular hydrogen transfer followed by ring fission. The resultant ion loses CH3CN in a subsequent step. The origin of many fragment ions was traced with the use of B/E linked-scan spectra.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/oms.1210220407
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  • 7
    Electronic Resource
    Electronic Resource
    K-absorption edges of cobalt in its metallic soaps and their liquid crystalline solutions in benzene (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    X-Ray Spectrometry 14 (1985), S. 136-138 
    Details
    ISSN: 0049-8246
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: X-ray K-absorption edges of cobalt in some metallic soaps (myristate, palmitate, stearate and oleate) and their solutions in benzene have been recorded. The latter are liquid crystals. The bond length between the metal and the ligand was found to be greater in solutions than in the solids.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/xrs.1300140307
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  • 8
    Electronic Resource
    Electronic Resource
    13C NMR studies of the friedelane series of triterpenoids and the conformation of the D and E rings in friedelan-7-oneDedicated to Proof. Ajay K. Bose on his sixtieth birthday. (1987)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 25 (1987), S. 39-41 
    Details
    ISSN: 0749-1581
    Keywords: 13C NMR ; Friedelanes ; Triterpenoids ; D/E ring conformation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The 13C chemical shifts in the spectra of friedelan-7-one (1), methyl 3-oxofriedelan-25-oate (2), methyl friedelan-25-oate (3), 7-oxofriedelan-3-α-yl acetate (4), friedelan-3-one (5), 2-hydroxy-3, 4-seco-friedelan-3-oic acid (6), methyl 2-oxo-3,4-seco-friedelan-3-oate (7), methyl 2-hydroxy-3,4-seco-friedelan-3-oate (8) and 3,4-seco-friedelan-2,3-diol (9) have been fully assigned using two-dimensional heteronuclear correlation NMR spectroscopy, BB proton decoupled, DEPT and selective heteronuclear spin decoupling methods. These assignments and the observation of substantial NOEs between 26-methyl and 28-methyl and between 28-methyl and 30-methyl support the boat to boat conformations for the D and E rings in 1. Corrections and correlations are made of some earlier literature assignments of friedelanones.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260250110
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  • 9
    Electronic Resource
    Electronic Resource
    Improved 2D inverse proton detected C,H correlation NMR techniques for the total assignment of carbon resonances of a highly delta opioid receptor agonist peptide (1993)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 31 (1993), S. 231-237 
    Details
    ISSN: 0749-1581
    Keywords: Inverse experiments ; Long-range H,C correlations ; Delta opioid receptor agonist peptide ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Assignment of the 13C resonances of the highly delta opioid receptor selective cyclic peptide enkephalin (DPDPE) was accomplished by using single-quantum inverse proton detected 2D heteronuclear correlation spectroscopy. The pulse sequences described provided better sensitivity and resolution than the corresponding, commonly used, multiple-quantum evolution experiments, since during the SQ-evolution period there is no line broadening due to scalar and dipolar 1H-1H interactions. The proton detected C,H correlation spectrum, in combination with the H-TOCSY relay C,H correlation, gave the assignment of all protonated carbons. The C,H correlation optimized for long-range coupling gave the assignments of all non-protonated carbons, such as the carbonyl carbons of the peptide bonds. These spectra also allowed the sequential assignment of this molecule.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260310304
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  • 10
    Electronic Resource
    Electronic Resource
    Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 6 (1990), S. 363-366 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; Selectable markers ; Plasmids ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A set of plasmids was constructed that contain the yeast selectable markers HIS3, LEU2, TRP1 or URA3 embedded in the multiple cloning site of pUC18.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320060502
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