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  • Chemistry  (17)
  • Life and Medical Sciences  (7)
  • Cell & Developmental Biology  (5)
  • Aircraft Propulsion and Power
  • Fundamental concepts
  • Plasma membrane
  • Yarrowia lipolytica
  • 1990-1994  (14)
  • 1985-1989  (12)
Collection
Keywords
Publisher
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Replacement of the promoter of the yeast plasma membrane ATPase gene by a galactose-dependent promoter and its physiological consequences (1987)
    Springer
    Current genetics 12 (1987), S. 105-110 
    Details
    ISSN: 1432-0983
    Keywords: Yest ; ATPase ; Galactose promoter ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to probe the physiological role of the yeast plasma membrane ATPase we have replaced the constitutive promoter of its gene by a galactose-dependent promoter. The resulting cells stop growing on glucose medium when the preformed ATPase is diluted to 20% of normal. There is a correlation between ATPase activity and both proton efflux from the cells and amino acid transport. A large proportion of growth-arrested cells appear enlarged and with several buds containing nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00434664
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  • 2
    Electronic Resource
    Electronic Resource
    Cell wall composition of the yeast and mycelial forms of Yarrowia lipolytica (1986)
    Springer
    Archives of microbiology 144 (1986), S. 124-130 
    Details
    ISSN: 1432-072X
    Keywords: Dimorphism ; Yarrowia lipolytica ; Cell wall composition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cell walls of the yeast and mycelial forms of Yarrowia lipolytica were isolated and purified. Electron microscopy studies showed no differences between both types of cell walls. Chemical analysis revealed that the yeast cell wall contained 70% neutral carbohydrate, 7% amino sugars, 15% protein, 5% lipids and 0.8% phosphorus. Mycelial cell walls contained 70% carbohydrate, 14% aminosugars, 6% protein, 5% lipids and 0.6% phosphorus. Three polysaccharides: β-glucan, mannan and chitin were detected. Proteins were solubilized from both cell wall fractions and separated by polyacrylamide gel electrophoresis. About 50 protein bands were detected, four of them corresponding to glycoproteins. The cell walls of the yeast and mycelial forms of Y. lipolytica were qualitatively similar and only quantitative differences were found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00414721
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  • 3
    Electronic Resource
    Electronic Resource
    Preparation of [5 + 6]-, [6 + 6]-, and [6 + 7]-Bicyclic Guanidines from C,C'-Bis(iminophosphoranes) (1994)
    Weinheim : Wiley-Blackwell
    Berichte der deutschen chemischen Gesellschaft 127 (1994), S. 1641-1652 
    Details
    ISSN: 0009-2940
    Keywords: Bis(iminophosphoranes) ; Guanidines, bicyclic ; Aza Wittig reaction ; Chemistry ; Inorganic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A one-pot synthesis of [5 + 6]-, [6 + 6]-, and [6 + 7]-rigid bicyclic guanidines (6, 8, 19, 21, 23) based on a bis(iminophosphorane)-mediated annulation strategy is reported. The crystal and molecular structure of the parent [5 + 6]-bicyclic guanidine 8 has been established by X-ray diffraction. The crystallographically independent molecule forms dimers through a centre of symmetry by N-H-N hydrogen bonds. The molecule, and therefore the dimer, is statistically disordered in a 65/35 ratio. A statistical study of N-H-N hydrogen bonds of this kind of dimers has been carried out by using the Cambridge Structural Database.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cber.19941270916
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  • 4
    Electronic Resource
    Electronic Resource
    Acid and base functionalization of ethylene-propylene rubbers, 1. Grafting of tertiary amino groups and “interpolymer” network formation (1987)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 188 (1987), S. 951-960 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Base functionalization of saturated ethylene-propylene rubbers (EPR) was performed either by means of the radical grafting of 2-(dimethylamino)ethyl methacrylate onto EPR or by the reaction of a succinic anhydride grafted EPR with N,N-dimethylethylenediamine. Differences in the structure of the grafted molecules and in their thermal stability are also reported. An investigation is made on the reactivity of EPR having tertiary amino pendant groups toward acid functionalized EPR leading to gelation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1987.021880502
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  • 5
    Electronic Resource
    Electronic Resource
    Dynamic mechanical relaxations in a copolyterephthalate based on ethylene glycol and 1,4-cyclohexanedimethanol (1988)
    New York : Wiley-Blackwell
    Die Makromolekulare Chemie 189 (1988), S. 1207-1217 
    Details
    ISSN: 0025-116X
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The dynamic mechanical relaxations of poly(ethylene terephthalate) (PET), poly(1,4-cyclo-hexylenedimethylene terephthalate) (PCHDMT), and a copolymer of them (COP) were measured in the temperature range from -150 to 150°C by use of a dynamic viscoelastometer. The viscoelastic relaxations of COP are studied and compared with those of PET and PCHDMT. The anisotropy of the viscoelastic properties of stretched COP samples was also studied in order to distinguish between main and secondary relaxations.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/macp.1988.021890522
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  • 6
    Electronic Resource
    Electronic Resource
    The antibiotic complex of retamycins: A fast atom bombardment mass spectrometric study (1989)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 18 (1989), S. 553-557 
    Details
    ISSN: 0887-6134
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Fast atom bombardment (FAB) mass spectrometry is, for the first time, successfully applied to the underivatized anthracycline antibiotics retamycins E1 and E2, using bis-(2-hydroxyethyl)sulphide as the matrix compound. The spectra are characterized by abundant protonated molecular ions and also by a number of sequence ions, which can be easily rationalized in terms of the carbohydrate sequence in the molecules. The relative abundance of these ions seems to depend on the functionalities close to the glycosidic oxygen, and also on their stereochemical relationship. Structural results are also confirmed by the analysis of the FAB mass spectra of the acetyl derivatives.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200180807
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  • 7
    Electronic Resource
    Electronic Resource
    Broadband dielectric spectroscopy on an oxovanadium metallomesogen compoundThis work was supported by the CICYT (Spain, project MAT-91-0962-C02-02) and the EC Human Capital and Mobility Programme (ERB4050 PL922749). (1994)
    Weinheim : Wiley-Blackwell
    Advanced Materials 6 (1994), S. 941-944 
    Details
    ISSN: 0935-9648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/adma.19940061208
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  • 8
    Electronic Resource
    Electronic Resource
    Use of anti-idiotypic antibodies as probes for the interaction of TSH subunits with its receptor (1987)
    New York, N.Y. : Wiley-Blackwell
    Journal of Cellular Biochemistry 34 (1987), S. 151-162 
    Details
    ISSN: 0730-2312
    Keywords: anti-idiotypic antibodies ; thyrotropin subunits ; thyrotropin receptor ; monoclonal antibody ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: TSH is a heterodimeric glycoprotein hormone, whose dissociated subunits are without biological activity. This has precluded the assessment of the relative contribution of each subunit to hormone action. We have raised anti-idiotypes to monoclonal antibodies specific, respectively, for the α and β hTSH subunits. The anti-β anti-idiotype inhibited l25I-hTSH binding to the β subunit-specific monoclonal quantitatively, whereas 125I-hTSH binding to the α subunit-specific monoclonal was not inhibited by anti-α anti-idiotypes, suggesting that only the former is an “internal image” anti-idiotype. Neither of the two anti-idiotypes nor equimolar mixtures thereof inhibited 125I-bTSH binding to thyroid membranes, even though radiolabelled anti-idiotypes showed saturable binding to thyroid plasma membrane which was inhibited 41-65% by bTSH. Each anti-idiotype alone caused 9% inhibition (compared to 50% by NRIgG) of thyroid plasma membrane adenylate cyclase. Equimolar mixtures (125 μg/ml IgG of each anti-idiotype) induced enzyme activity equivalent to 85% of that of 250 mU/ml of TSH. The TSH-like action of the two anti-idiotypes was also reflected in their capacity to increase (450% by 250 μg/ml IgG compared to normal rabbit IgG) the uptake of 131I into isolated thyrocytes and to promote the organization of such cells into follicular structures. At 250 μg/ml, anti-β anti-idiotype promoted the organization of small follicles and only at a concentration of 500 μg/ml did it enhance 131I uptake. Anti α idiotype was without effect in both assays. Lastly, mixture of anti-idiotypes bound to the ∼ 197,000 Mr band (TSH holoreceptor) on protein blots of thyroid plasma membranes resolved on NaDSO4-polyacrylamide gel electrophoresis under non-reducing conditions. Individual anti-idiotypes were without effect.The TSH α and β subunits apparently deliver two cooperative signals to the receptor and that specificity is associated with the β subunit, while the α subunit is important in enhancing receptor affinity for the heterodimer and in stablizing TSH-receptor complex.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcb.240340302
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  • 9
    Electronic Resource
    Electronic Resource
    Behaviour of a silica filler surface-modified with boron-amine groups (1994)
    Weinheim : Wiley-Blackwell
    Angewandte Makromolekulare Chemie 217 (1994), S. 107-117 
    Details
    ISSN: 0003-3146
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Das Verhalten von mit primären Boramingruppen modifizierten Silikat-Füllstoffen wurde unter verschiedenen Reaktionsbedingungen untersucht. Der Füllstoff mit den besten Verstärkungseigenschaften wurde eingesetzt, um zwei unterschiedliche Vulkanisationssysteme zu vergleichen: ein wirksames Vulkanisationsverfahren (EV), das nach ISO als Methode zur Untersuchung des Verstärkungsverhaltens neuer Silikate empfohlen wird, sowie ein konventionelles Verfahren. Die besten Ergebnisse werden mit Silikat erhalten, welches 3 h bei 500°C behandelt wurde; unter diesen Bedingungen werden die meisten der primären Boramingruppen fixiert. Die Eigenschaften des nach dem EV-Verfahren modifizierten Silikats ähneln denen von mit Silan modifizierten Silikaten und sind in einigen Fallen besser als jene, welche nach dem konventionellen Verfahren erhalten werden. In allen Fallen wird die verstarkende Wirkung von gefällten Silikat-Füllstoffen durch die Boraminbehandlung verbessert.
    Notes: The behaviour of silica fillers modified with primary boron-amine groups has been studied under different reaction conditions. The filler with the best reinforcing properties was used to compare two different vulcanization systems: an efficient vulcanization system, EV, which is the recommended ISO Standards method to study the reinforcing behaviour of new silicas, and a conventional vulcanization system.The results indicate that the best properties are obtained with silica treated for 3 h at 500°C, reaction conditions which fix most of the primary boron-amine groups. Using the EV system, the properties of this modified silica are similar to those of silane-modified silica, and in some cases better than those obtained with a conventional vulcanization system. In all cases, the boron-amination treatment significantly improves the behaviour of precipitated silica as a reinforcing filler.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/apmc.1994.052170111
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  • 10
    Electronic Resource
    Electronic Resource
    Targeting of aequorin for calcium monitoring in intracellular compartments (1994)
    New York : Wiley-Blackwell
    Journal of Bioluminescence and Chemiluminescence 9 (1994), S. 177-184 
    Details
    ISSN: 0884-3996
    Keywords: Photoproteins ; calcium ; organelles ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: We have recently developed a new method for monitoring Ca2+ concentrations in defined cell compartments. The cDNA encoding the Ca2+-sensitive photoprotein aequorin has been modified in order to include specific targeting sequences and expressed in eukaryotic cells; the recombinant protein, specifically located inside the cells, has allowed the direct study of mitochondrial and nuclear Ca2+ concentrations in living cells. The principles, and the application, of this new methodology are discussed in this article.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bio.1170090312
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