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  • 25.85.Ge  (3)
  • Saccharomyces cerevisiae  (3)
  • 1990-1994  (3)
  • 1985-1989  (3)
Sammlung
Schlagwörter
Verlag/Herausgeber
Erscheinungszeitraum
  • 1990-1994  (3)
  • 1985-1989  (3)
Jahr
  • 1
    Digitale Medien
    Digitale Medien
    Effects of m-Cl-peroxy benzoic acid on glycolysis in Saccharomyces cerevisiae (1985)
    Springer
    Archives of microbiology 143 (1985), S. 220-224 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Peroxy benzoic acid ; Saccharomyces cerevisiae ; ATP ; Glycolysis ; Glyceraldehyde-3-phosphate dehydrogenase ; Colony forming capacity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Concentrations of m-Cl-peroxy benzoic acid (CPBA) higher than 0.1 mM decrease the ATP-content of Saccharomyces cerevisiae in the presence of glucose in 1 min to less than 10% of the initial value. In the absence of glucose, 1.0 mM CPBA is necessary for a similar effect. After the rapid loss of ATP in the first min in the presence of glucose caused by 0.2 mM CPBA, the ATP-content recovers to nearly the initial value after 10 min. Aerobic glucose consumption and ethanol formation from glucose are both completely inhibited by 1.0 mM CPBA. Assays of the activities of nine different enzymes of the glycolytic pathway as well as analysis of steady state concentrations of metabolites suggest that glyceraldehyde-3-phosphate dehydrogenase is the most sensitive enzyme of glucose fermentation. Phosphofructokinase and alcohol dehydrogenase are slightly less sensitive. Incubation for 1 or 10 min with concentrations of 0.05 to 0.5 mM CPBA causes a) inhibition of glyceraldehyde-3-phosphate dehydrogenase, b) decrease of the ATP-content and c) a decrease of the colony forming capacity. From these findings it is concluded that the disturbance of the ATP-producing glycolytic metabolism by inactivation of glyceraldehyde-3-phosphate dehydrogenase may be an explanation for cell death caused by CPBA.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00411239
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Effect of ozone on ATP, cytosolic enzymes and permeability of Saccharomyces cerevisiae (1987)
    Springer
    Archives of microbiology 147 (1987), S. 105-108 
    Details
    ISSN: 1432-072X
    Schlagwort(e): Ozone ; Yeast ; Saccharomyces cerevisiae ; ATP ; Nucleotides ; Permeability ; Cytosolic enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Treatment of a yeast suspension with ozone inactivates a number of cytosolic enzymes. Among 15 studied, the most drastic inactivation was found for glyceraldehyde-3-phosphate dehydrogenase and to lesser extents: NAD-glutamate dehydrogenase, pyruvate decarboxylase, phosphofructokinase-1 and NAD-alcohol dehydrogenase. Ozone treatment also effects the quantity of ATP and of other nucleoside triphosphates, reducing to about 50% of the initial value. The ATP missing in the cells appears in the medium. NAD and protein also accumulate in the medium suggesting that the yeast cells have been permeabilized. Permeabilization of the yeast cells by treatment with ozone preceeds the inactivation of glyceraldehyde-3-phosphate dehydrogenase and other cytosolic enzymes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00415269
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Mass resolved angular distribution in helium ion induced fission of233U (1992)
    Springer
    The European physical journal 342 (1992), S. 299-301 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 25.85.Ge ; 25.85.Ee
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Mass resolved fission fragment angular distribution was measured in the 28.5 MeV alpha induced fission of233U using recoil catcher technique and direct gamma spectrometry. The angular distribution of 8 fission products were obtained. The angular anisotropies of asymmetric fission products were found to be higher than those of symmetric products indicating a correlation between the fragment angular distribution and the mass distribution.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01291513
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Fragment angular momenta in low and medium energy fission of242Pu (1987)
    Springer
    The European physical journal 327 (1987), S. 225-229 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 25.85.Ge ; 25.85.Ec
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Independent isomeric yield ratios of128Sb were determined radiochemically in the thermal neutron induced fission of241Pu and 34 MeV alpha particle induced fission of238U, both involving the same compound nucleus (242Pu). Fragment angular momenta estimated from the measured isomer ratios using the statistical model analysis showed significantly larger fragment angular momenta in the medium energy fissioning system compared to the low energy fissioning system. This has been attributed to the effect of higher excitation energy and angular momentum in the entrance channel leading to increased fragment temperature, moments of inertia and angular velocity. An attempt was made to calculate the fragment angular momentum in the medium energy fission using the Fermi gas model for the fissioning nucleus, taking into account the multichance fission, saddle shapes of the fissioning nuclei and the angular velocity components of the fissioning nuclei both along and orthogonal to the fission axis. The calculated angular momenta agree well with the experimental results.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01292412
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Effect of entrance channel parameters on fission fragment angular momentum in medium energy fission (1992)
    Springer
    The European physical journal 342 (1992), S. 95-100 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 25.70.W ; 25.85.Ge
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Independent isomeric yield ratios of132I were radiochemically determined in alpha particle induced fission of238U in the energy range 25–44 MeV. Fission fragment angular momenta were deduced from the measured isomeric yield ratios using spin dependent statistical model analysis. It was seen that angular momentum of132I increases with increase of excitation energy and angular momentum of the fissioning nucleus. Comparison of the present data on132I in238U(α,f) with the literature data for the same product in238U(p, f) and238U(γ, f) at various excitation energies show that fragment angular momentum strongly depends on the input angular momentum in the range of excitation energy considered. Experimental fragment angular momentum at all excitation energies were seen to be in agreement with the theoretical values calculated based on thermal equilibration of the various collective rotational degrees after considering the occurence of multichance fission. Thus, strong effect of input angular momentum as well as the statistical equilibration among the various collective rotational degrees of freedom in medium energy fission is corroborated.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01294493
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 6 (1990), S. 363-366 
    Details
    ISSN: 0749-503X
    Schlagwort(e): Saccharomyces cerevisiae ; Selectable markers ; Plasmids ; Life and Medical Sciences ; Genetics
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie
    Notizen: A set of plasmids was constructed that contain the yeast selectable markers HIS3, LEU2, TRP1 or URA3 embedded in the multiple cloning site of pUC18.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/yea.320060502
    Standort Signatur Erwartet Verfügbarkeit
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