Publication Date:
1994-07-01
Description:
The SWI/SNF protein complex is required for the enhancement of transcription by many transcriptional activators in yeast. Here it is shown that the purified SWI/SNF complex is composed of 10 subunits and includes the SWI1, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products. The complex exhibited DNA-stimulated adenosine triphosphatase (ATPase) activity, but lacked helicase activity. The SWI/SNF complex caused a 10- to 30-fold stimulation in the binding of GAL4 derivatives to nucleosomal DNA in a reaction that required adenosine triphosphate (ATP) hydrolysis but was activation domain-independent. Stimulation of GAL4 binding by the complex was abolished by a mutant SWI2 subunit, and was increased by the presence of a histone-binding protein, nucleoplasmin. A direct ATP-dependent interaction between the SWI/SNF complex and nucleosomal DNA was detected. These observations suggest that a primary role of the SWI/SNF complex is to promote activator binding to nucleosomal DNA.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Cote, J -- Quinn, J -- Workman, J L -- Peterson, C L -- GM47867-02/GM/NIGMS NIH HHS/ -- GM49650-01/GM/NIGMS NIH HHS/ -- R01 GM049650/GM/NIGMS NIH HHS/ -- R37 GM049650/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 1994 Jul 1;265(5168):53-60.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park 16802.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8016655" target="_blank"〉PubMed〈/a〉
Keywords:
Adenosine Triphosphatases/metabolism
;
Adenosine Triphosphate/metabolism
;
Base Sequence
;
DNA Helicases/metabolism
;
DNA Probes
;
DNA, Fungal/*metabolism
;
DNA-Binding Proteins/genetics/metabolism
;
Deoxyribonuclease I/metabolism
;
Fungal Proteins/*metabolism
;
Gene Expression Regulation, Fungal
;
Molecular Sequence Data
;
Nuclear Proteins/metabolism/pharmacology
;
Nucleoplasmins
;
Nucleosomes/*metabolism
;
*Phosphoproteins
;
Saccharomyces cerevisiae/genetics/metabolism
;
*Saccharomyces cerevisiae Proteins
;
Transcription Factors/genetics/isolation & purification/*metabolism
;
Transcription, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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