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  • Rat  (2)
  • Springer  (2)
  • Blackwell Publishing Ltd
  • Springer Nature
  • 1990-1994  (1)
  • 1985-1989  (1)
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  • Springer  (2)
  • Blackwell Publishing Ltd
  • Springer Nature
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  • 1990-1994  (1)
  • 1985-1989  (1)
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  • 1
    ISSN: 1432-0878
    Keywords: Ascites hepatoma ; Adhesive factor ; Monoclonal antibody ; Cell membrane polarity ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract A cell surface-associated adhesive factor (AF) separated from differentiated rat ascites hepatoma AH136B cells (forming cell islands in vivo) has been highly purified by chromatography. AF is assumed to mediate the cell-cell adhesion essential to island formation of the hepatoma cells. A substance, immunologically crossreactive with AF, is present in the ascites fluid or culture medium of the AH136B cells. Because the substance is almost identical to AF in molecular weight and aggregation-promoting activity, it has been concluded that AF is released into the ascites fluid where it is concentrated. Monoclonal antibodies have been raised against AF purified from ascites fluid of AH136B cells. We have obtained a monoclonal antibody, coded MoAF-6D6, that strongly abolishes the aggregation-promoting activity of AF. When AH136B cell islands are incubated in the presence of Fab fragments of MoAF-6D6, cell detachment from the islands is evident within 24 h. Cell islands following 36-h culture show a distinct dissociation and islands completely lose their organization 48 h after culture. The dissociating effect of MoAF-6D6 is neutralized by the addition of AF. These results suggest that AF plays a significant role in the maintenance of cell islands.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 25 (1987), S. 386-390 
    ISSN: 1741-0444
    Keywords: Central caudal artery ; Electrical impedance plethysmography ; Electromagnetic flowmetry ; Peripheral blood flow ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The impedance Z, the derivative of Z with respect to time t, dZ/dt, and blood flow F have been measured simultaneously in the central caudal artery of a series of anaesthetised rats. The results of the experiments were expressed in the relationship derived from the Windkessel theory where f(t)=adz(t)/dt+βz(t), in which α and β are constants and f(t) and z(t) express the pulsating fractions in the blood flow F and the impedance Z, respectively. The correlation between the peak-to-peak values of f(t) and of adz(t)/dt+βz(t) was 0·96. The correlation between the mean flow fo and the peak-to-peak value of f(t) was 0·74. Furthermore, even when the mean flow was zero, adz(t) still existed. The theoretical model was tested against volume flow measurements made using an electromagnetic flowmeter and was found to correlate well. Theoretical estimates of vessel calibre also accord with measured values.
    Type of Medium: Electronic Resource
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