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  • Protein body  (2)
  • Sporotrichosis  (2)
  • Springer  (4)
  • Blackwell Publishing Ltd
  • Springer Nature
  • 1990-1994  (2)
  • 1985-1989  (2)
Collection
Keywords
Publisher
  • Springer  (4)
  • Blackwell Publishing Ltd
  • Springer Nature
Years
  • 1990-1994  (2)
  • 1985-1989  (2)
Year
  • 1
    Electronic Resource
    Electronic Resource
    Histopathological studies ofSporothrix schenckii-inoculated mice (1993)
    Springer
    Mycopathologia 122 (1993), S. 89-93 
    Details
    ISSN: 1573-0832
    Keywords: Congenitally athymic nude mouse ; Polymorphonuclear leukocytes ; Sporotrichosis ; Sporothrix schenckii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Defense mechanisms againstSporothrix schenckii were studied using mouse models. After an intracutaneous injection of the yeast form ofS. schenckii to the dorsal skin of the congenitally athymic nude and normal heterozygote littermate mice, nodules were formed. They regressed and disappeared in 10 weeks in the case of normal mice. On the other hand, nodules and then ulceration developed progressively in nude mice until all animals expired by dissemination of microorganisms at the 11th week of inoculation. Histopathologically the migrated cells were similar in both the normal and the nude mice, particularly during the early phase (within 24 h), with infiltration by PMNs being predominant. Fragmentation ofS. schenckii commenced early during the 12–24 h stage of inoculation in the normal mice, while such fragmentation was scarce in nude mice even though numerous PMNs accumulated. Microscopic observations in the early stages (within 24 h of inoculation) suggested that the lack of killing activity by PMNs in nude mice contributes more to the impaired defense than the lack of macrophage activation by T-cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01103604
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of proteinase inhibitors on the cutaneous lesion ofSporothrix schenckii inoculated hairless mice (1993)
    Springer
    Mycopathologia 123 (1993), S. 81-85 
    Details
    ISSN: 1573-0832
    Keywords: Chymostatin ; Pepstatin ; Proteinase inhibitor ; Sporotrichosis ; Sporothrix schenckii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sporothrix schenckii produces two extracellular proteinases, namely proteinase I and II. Proteinase I is a serine proteinase, inhibited by chymostatin. On the other hand, proteinase II is an aspartic proteinase, inhibited by pepstatin. The addition of either pepstatin or chymostatin to the culture medium did not inhibit cell growth, however the addition of both inhibitors strongly inhibited fungal growth. Accordingly, this suggested that extracellular proteinases play an important role in cell growth and that such cell growth may be suppressed if these proteinases are inhibited. In order to substantiate this speculation in sporotrichosis, the effects of proteinase inhibitors on the cutaneous lesions of mice were studied. Ointments containing 0.1% chymostatin, 0.1% pepstatin and 0.1% chymostatin-0.1% pepstatin were applied twice daily on the inoculation sites of hairless mouse skin, and the time courses of the lesions examined. The inhibitory effect in vivo onS. schenckii was similar to that demonstrated in our previous in vitro study. Compared to the control, the time course curve of the number of nodules present after the application of either pepstatin or chymostatin was slightly suppressed. The application of both pepstatin and chymostatin, however, strongly suppressed nodule formation. This study not only confirmed the role of 2 proteinases ofS, schenckii for fungal growth in vivo, but also may lead to their use as new topical therapeutic agents.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01365084
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  • 3
    Electronic Resource
    Electronic Resource
    Mutants for rice storage proteins (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 305-310 
    Details
    ISSN: 1432-2242
    Keywords: Endosperm ; Mutant ; Oryza sativa L. ; Protein body ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Rice storage proteins of the endosperm are localized in two types of protein bodies, PB-I and PB-II. Protein bodies were isolated by sucrose density gradient centrifugation from developing endosperm of three rice mutants, CM 21, CM 1675 and CM 1834, and characterized after pepsin-digestion treatment by protein contents determination. Mutant protein bodies (PBs) except for their internal structure, were similar in shape and density to PB-I of the variety Kinmaze. Electrophoretic analysis of PB-I polypeptides revealed that SDS (Sodium dodecylsulfate) bands of 13 and 16 kilodaltons consisted, respectively, of four and two individual polypeptides with different pI values, while the 10-kilodalton band behaved as a single polypeptide after isoelectric focussing (IEF) electrophoresis. The differences in the polypeptide composition induced by mutants were due to the decrease and/or increase in the content of specific PB-I polypeptides. Electron microscopic observations revealed that the typical lamellar structure of the PB-I is not visible in CM 1675. On the contrary, the inner portion of PB-I in CM 1834 and CM 21 showed higher electron density than that of the variety Kinmaze. On these two mutants, the content of pepsin-indigestible and -digestible proteins were similar to those of Kinmaze, although the values of the PB-II/PB-I ratio were greater than those for Kinmaze, suggesting that these two mutants are high-glutelin rice mutants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265288
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  • 4
    Electronic Resource
    Electronic Resource
    Mutants for rice storage proteins (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 11-16 
    Details
    ISSN: 1432-2242
    Keywords: Endosperm ; Mutant ; Oryza sativa L. ; Protein body ; Storage protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To obtain genetic materials to breed qualitatively improved rice storage proteins, we screened about 3,000 mutant lines induced by the treatment of rice fertilized egg cell with N-methyl-N-nitrosourea (MNU). The screening was performed by comparing the profiles of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) with that of the original variety, Kinmaze, especially focussing on the changes in polypeptides present in two kinds of protein bodies, PB-I and PB-II. We selected 17 mutant lines and classified them into 4 types on the basis of variations of the relative contents of the polypeptides. Determination of extracted protein in the starchy endosperm of the mutants revealed changes in the content of prolamin and glutelin but not globulin. In some mutants there was marked accumulation of 57 kDa polypeptide concomitant with the remarkable reduction of glutelin subunits. Treatment of the fertilized egg cell with MNU was found to be an effective method to induce mutations for storage proteins in protein bodies of rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288825
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