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  • 1
    ISSN: 1432-2242
    Keywords: Key words NESTUR ; Stem growth efficiency ; RAPD ; QTL ; Haploid megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  NESTUR (needle-to-stem unit rate) is a stem growth index of conifer seedlings that measures the efficiency of stemwood production per unit of needle growth, and is related to other seedling traits such as height, stem diameter, stem volume and needle volume. Quantitative trait loci (QTLs) affecting the expression of stem growth efficiency in radiata pine seedlings were investigated using a RAPD linkage map constructed from markers scored on haploid, megagametophytic DNA. Four putative QTLs were detected which accounted for 8.5–36.4% of the population variance. A search for evidence of epistasis, using both complete pairwise and conditional interactions, did not yield any statistically significant result. Over a 3-year period, seedlings with high-NESTUR marker alleles showed a superior growth performance of 17–40% for height, diameter and volume over those with low-NESTUR marker alleles.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 97 (1998), S. 1053-1061 
    ISSN: 1432-2242
    Keywords: Key words RAPD ; QTL ; Age effects ; Stem growth ; Linkage map ; Haploid megagametophyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Haploid megagametophytes from a full-sib cross of Pinus radiata were used to construct a genetic linkage map for radiata pine based on random amplified polymorphic DNA (RAPD) markers. The map, which was made from 222 markers, was used to carry out a QTL analysis of growth using measurements made on seedlings from which these megagametophytes were collected at an early germination stage. Trends in the expression of QTLs for stem diameter, volume and height were compared using measurements made at 5 months of age, and at 1, 2 and 3 years of age. None of the observed trends showed complete stability, i.e. none of the putative QTL positions detected at any one age was strongly expressed at all of the four stages of measurement. However, 45% of the trends showed partial stability, i.e. putative QTLs significant at one age were also detected at a subsequent age. Trends in QTL expression with age followed one of three patterns: (1) putative QTLs at some locations showed a gradual linear increase in influence from 5 months of age and were highest at 3 years of age; (2) QTLs detected at 5 months of age gradually became less significant with age; and (3) some putative QTLs showed a curvilinear increase in effect from 5 months of age, reaching their peak expression at 1 to 2 years, and sometimes were still detected at 3 years of age.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 95 (1997), S. 119-124 
    ISSN: 1432-2242
    Keywords: Key words Bulked segregant analysis ; Quantitative trait locus ; Epistasis ; Stem growth efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Needle-to-stem unit rate (NESTUR) is a stem growth index of conifer seedlings that measures the efficiency of stemwood production per unit of foliage growth. The random amplified polymorphic DNA (RAPD) technique was applied to haploid DNA from the megagametophytes of a full-sib radiata pine cross to find markers linked to factors controlling the NESTUR trait. Using the bulked segregant analysis approach, 23 of 933 primers displayed putative linkage to factors controlling NESTUR. Based on the genotypic analysis of 174 individuals, two quantitative trait loci (QTLs) controlling NESTUR were identified at ANOVA P-levels of 0.01–0.001. The QTLs were identified by RAPD markers OPE-06450 and OPA-101200, which were linked to each other (r=7%), and UBC-333550, which was not linked to the other two. Linkage to components of NESTUR (increments in stem diameter and stem volume) was demonstrated for UBC-333550, while the others were not linked to NESTUR components.
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  • 4
    ISSN: 1573-5095
    Keywords: Tea tree oil ; genetic parameters ; combined index selection ; breeding strategy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Variation in oil yields from plantations and natural stands of Melaleuca alternifolia (Maiden and Betche) Cheel indicates considerable potential for improving plantation production through breeding. Some populations of the more widely distributed species, M. linariifolia Sm., produce a similar leaf oil and may provide opportunities to expand the genetic base of plantations. Growth and oil traits were assessed for 60 half-sib families, representing three chemical forms of Melaleuca alternifolia, and 10 half-sib families of the terpinen-4-ol rich chemical form of M. linariifolia, in a provenance/progeny trial in northern New South Wales, Australia. Differences in oil composition and coppicing between the terpinen-4-ol rich forms of the two species were significant (P 〈 0.05), while differences in growth traits and leaf oil yield were not significant. The difference in oil composition between northern and southern populations of M. linariifolia was as great as differences between the two species, suggesting the two regions should be considered distinct races for breeding purposes. Narrow-sense heritabilities in M. alternifolia of 0.67 for oil yield, 0.25 for plant dry weight, and 0.27 for coppice indicate substantial improvement would follow selection for single traits. However, negative genetic correlations between oil yield and plant dry weight indicate it is not possible to simultaneously achieve major gains in the two traits. Using combined index selection with a restriction on plant dry weight, expected gains of 17% in oil yield and 14% in coppicing are predicted from one generation of selection at an intensity of one tree in ten.
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