ISSN:
1573-5060
Keywords:
Oryza sativa L.
;
opaque endosperm
;
Wx alleles
;
amylose content
;
tissue specificity
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Amylose content is a major determinant of the eating quality in rice. To elucidate the allelic diversity at the Waxy (Wx) gene which controls the amylose synthesis, two cultivated strains having opaque endosperms were studied. The gene responsible for opaque endosperms was introduced into the genetic background of the Japonica type of rice by successive backcrosses, and the two near-isogenic lines (NILs) were selected from the B5 generation. The genetic experiments revealed that an allele, Wxop, controls opaque endosperms which show chalky as wx endosperms in spite of the production of amylose. Immunoblotting analysis was carried out to compare the gene expression by using the NILs with 4 different alleles (Wxa, Wxb, Wxop and wx). The level of the gene product bound to starch granules was slightly lower in the NILs with Wxop than that with Wxb, showing a positive correlation with amylose content in the endosperm. Extracts from mature anthers indicated that the gene product was markedly reduced in the NILs with Wxop as well as that with wx, showing an altered expression in the tissue specificity in the Wxop lines. Sequence analysis suggested that the Wxop had been derived from Wxa, independently of the origin of Wxb. The importance of the gene regulation was discussed in relation to diversified phenotypes established during the domestication process.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1003457209225
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