ISSN:
1573-4919
Keywords:
Leishmania donovani
;
promastigotes
;
metabolites
;
nuclear magnetic resonance (NMR)
;
perchloric acid extract (PCA)
;
strain differentiation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
Notes:
Abstract Proton nuclear magnetic resonance spectroscopy was used for studying the intracellular metabolite profile of promastigotes of Leishmania donovani. The major intracellular metabolites observed in the promastigotes were acetate, alanine, succinate, glycine, α-glycerophosphorylcholine, acetoacetate, arginine and ethanol. A comparative study of the intracellular metabolite profile of promastigotes of different strains of L. donovani showed that, all the major intracellular metabolites were present in promastigotes of different strains. A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00250991
Permalink